Sarcophilus harrisii (Tasmanian devil) (Sarcophilus laniarius)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Metatheria; Dasyuromorphia; Dasyuridae; Sarcophilus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36286 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7N4UYB8|A0A7N4UYB8_SARHA Reverse transcriptase domain-containing protein OS=Sarcophilus harrisii OX=9305 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 4.75FQQLADD8 pKa = 5.0VADD11 pKa = 4.16KK12 pKa = 10.25WCSNTPFDD20 pKa = 6.16LIATEE25 pKa = 4.06EE26 pKa = 4.36TEE28 pKa = 4.33RR29 pKa = 11.84RR30 pKa = 11.84MDD32 pKa = 4.29FYY34 pKa = 11.47ADD36 pKa = 3.74PGVSFYY42 pKa = 10.98VLCPDD47 pKa = 4.64NGCGDD52 pKa = 3.96NFHH55 pKa = 6.3VWSEE59 pKa = 4.48SEE61 pKa = 3.97DD62 pKa = 3.63CLPFLQLAQDD72 pKa = 4.45YY73 pKa = 10.71ISSCGKK79 pKa = 8.56KK80 pKa = 7.94TLHH83 pKa = 6.31EE84 pKa = 4.11VLEE87 pKa = 4.47KK88 pKa = 10.61VFKK91 pKa = 10.63SFRR94 pKa = 11.84PLLGLPDD101 pKa = 3.99IDD103 pKa = 5.13DD104 pKa = 4.51DD105 pKa = 4.79AFEE108 pKa = 4.79EE109 pKa = 4.52YY110 pKa = 10.54SADD113 pKa = 3.7VEE115 pKa = 4.25EE116 pKa = 5.43DD117 pKa = 3.44EE118 pKa = 5.77PEE120 pKa = 4.67ADD122 pKa = 3.57HH123 pKa = 6.41QQMGVSQQQ131 pKa = 2.96

Molecular weight: 14.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7N4NFJ7|A0A7N4NFJ7_SARHA Endo/exonuclease/phosphatase domain-containing protein OS=Sarcophilus harrisii OX=9305 PE=4 SV=1
MM1 pKa = 7.13ARR3 pKa = 11.84YY4 pKa = 9.14RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84SRR9 pKa = 11.84SRR11 pKa = 11.84SRR13 pKa = 11.84SRR15 pKa = 11.84YY16 pKa = 6.95RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84SRR25 pKa = 11.84GRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84TYY32 pKa = 9.45RR33 pKa = 11.84RR34 pKa = 11.84SRR36 pKa = 11.84RR37 pKa = 11.84HH38 pKa = 3.62SRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84GRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84GYY49 pKa = 8.77SRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84YY54 pKa = 8.52SRR56 pKa = 11.84RR57 pKa = 11.84GRR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84YY62 pKa = 9.66

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19171

17115

36286

23604767

13

8801

650.5

72.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.364 ± 0.012

2.124 ± 0.011

4.84 ± 0.009

7.336 ± 0.018

3.723 ± 0.009

6.305 ± 0.019

2.56 ± 0.006

4.773 ± 0.011

6.215 ± 0.018

9.827 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.005

3.957 ± 0.009

6.055 ± 0.018

4.816 ± 0.015

5.418 ± 0.011

8.528 ± 0.017

5.337 ± 0.011

5.77 ± 0.01

1.154 ± 0.005

2.696 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski