Lobosporangium transversale
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11597 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y2GFQ1|A0A1Y2GFQ1_9FUNG DUF3533 domain-containing protein OS=Lobosporangium transversale OX=64571 GN=BCR41DRAFT_424219 PE=4 SV=1
MM1 pKa = 8.26 RR2 pKa = 11.84 YY3 pKa = 5.43 TTYY6 pKa = 10.79 TVTLCLLFALLASAAPVPEE25 pKa = 4.2 EE26 pKa = 3.76 TQEE29 pKa = 4.59 QKK31 pKa = 11.24 DD32 pKa = 4.33 CLDD35 pKa = 3.37 QCLTTEE41 pKa = 5.18 DD42 pKa = 3.8 EE43 pKa = 4.67 CLLNSISMSGCVTSYY58 pKa = 10.99 DD59 pKa = 3.19 EE60 pKa = 4.97 CYY62 pKa = 10.59 RR63 pKa = 11.84 MCIPTTTPTPTSVSAPAPTPGQGQGQPQPQPQPSIPVVSKK103 pKa = 9.85 NQKK106 pKa = 9.71 HH107 pKa = 6.28 KK108 pKa = 11.05 DD109 pKa = 3.51 VTHH112 pKa = 5.51 EE113 pKa = 4.3 TEE115 pKa = 3.79 ISSPTKK121 pKa = 10.65 KK122 pKa = 10.07 EE123 pKa = 3.98 DD124 pKa = 3.44 EE125 pKa = 4.67 KK126 pKa = 10.73 EE127 pKa = 4.46 TEE129 pKa = 4.21 EE130 pKa = 3.99 QTHH133 pKa = 5.58 EE134 pKa = 4.1 EE135 pKa = 4.27 EE136 pKa = 4.46 KK137 pKa = 11.19 DD138 pKa = 3.22 GDD140 pKa = 3.67 VDD142 pKa = 3.61 VDD144 pKa = 3.54 VDD146 pKa = 3.79 VDD148 pKa = 3.59 VDD150 pKa = 3.85 EE151 pKa = 4.99 EE152 pKa = 5.53 DD153 pKa = 3.77 GDD155 pKa = 4.24 FVEE158 pKa = 6.1 IPAPPGGEE166 pKa = 3.91 DD167 pKa = 3.69 DD168 pKa = 4.99 EE169 pKa = 5.92 EE170 pKa = 6.1 YY171 pKa = 11.09 DD172 pKa = 5.76 DD173 pKa = 5.52 EE174 pKa = 6.3 PSDD177 pKa = 3.89 YY178 pKa = 11.71
Molecular weight: 19.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.567
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.037
Thurlkill 3.605
EMBOSS 3.656
Sillero 3.884
Patrickios 0.846
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A1Y2GY89|A0A1Y2GY89_9FUNG CRAL-TRIO domain-containing protein OS=Lobosporangium transversale OX=64571 GN=BCR41DRAFT_331935 PE=4 SV=1
MM1 pKa = 6.81 TTLHH5 pKa = 6.34 LQFGLRR11 pKa = 11.84 PSHH14 pKa = 6.36 PLLRR18 pKa = 11.84 RR19 pKa = 11.84 VVLPPLLPKK28 pKa = 9.2 NTLMTQPQILITHH41 pKa = 7.58 LLPSTPIRR49 pKa = 11.84 LANANWDD56 pKa = 3.63 LPRR59 pKa = 11.84 SVNQRR64 pKa = 11.84 LPRR67 pKa = 11.84 NANRR71 pKa = 11.84 RR72 pKa = 11.84 LPKK75 pKa = 10.38 SKK77 pKa = 10.35 NWWLTRR83 pKa = 11.84 NVNRR87 pKa = 11.84 RR88 pKa = 11.84 LPRR91 pKa = 11.84 SAGWISIRR99 pKa = 11.84 ASWTALALNHH109 pKa = 6.24 SSARR113 pKa = 11.84 RR114 pKa = 11.84 IWVHH118 pKa = 5.86 PLFCIRR124 pKa = 11.84 TRR126 pKa = 11.84 FLMM129 pKa = 4.43
Molecular weight: 15.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.435
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.281
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.003
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.129
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11593
4
11597
5469817
49
5664
471.7
52.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.373 ± 0.02
1.244 ± 0.008
5.452 ± 0.017
6.131 ± 0.027
3.651 ± 0.017
5.972 ± 0.019
2.664 ± 0.013
5.485 ± 0.016
5.477 ± 0.022
8.966 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.429 ± 0.009
4.431 ± 0.014
5.341 ± 0.024
4.781 ± 0.026
5.444 ± 0.016
9.23 ± 0.036
6.208 ± 0.016
5.757 ± 0.018
1.104 ± 0.008
2.863 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here