Flavobacterium urocaniciphilum
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2554 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H8YT49|A0A1H8YT49_9FLAO Uncharacterized protein OS=Flavobacterium urocaniciphilum OX=1299341 GN=SAMN05444005_101160 PE=4 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.44 KK3 pKa = 10.55 LIFGALLSTLFMLFSCGGDD22 pKa = 3.32 DD23 pKa = 4.52 NNEE26 pKa = 4.15 TEE28 pKa = 4.25 NVLTGSMSATINGSSWSSTNGGAIANVMTVGFGGDD63 pKa = 3.53 SQKK66 pKa = 11.14 SLQIIGIASNTSSFTMNIPIEE87 pKa = 4.08 NLAVGSKK94 pKa = 9.07 TFSGDD99 pKa = 3.15 FAEE102 pKa = 5.0 GTLGYY107 pKa = 10.07 ISSNQMDD114 pKa = 4.4 VFTSQHH120 pKa = 4.73 TSGNFTVNITTLNLEE135 pKa = 4.2 TGKK138 pKa = 10.12 MSGTFSGTLYY148 pKa = 10.83 DD149 pKa = 5.04 DD150 pKa = 4.77 NDD152 pKa = 3.39 VSMSITNGVFTDD164 pKa = 3.84 VVFLSTDD171 pKa = 3.59 YY172 pKa = 11.13 YY173 pKa = 11.61 SNGSMSLKK181 pKa = 10.3 RR182 pKa = 11.84 NSGQVFTMDD191 pKa = 4.68 AFNEE195 pKa = 4.13 DD196 pKa = 3.52 GKK198 pKa = 11.2 FITIMEE204 pKa = 4.2 NSGDD208 pKa = 3.56 NSIAIMGNNANLTSDD223 pKa = 3.94 FGIYY227 pKa = 10.2 SLNFPKK233 pKa = 10.63 NIAAGTYY240 pKa = 9.93 ALTYY244 pKa = 10.58 DD245 pKa = 3.51 GTYY248 pKa = 10.41 NAGISNSNSQAEE260 pKa = 4.24 FTNVSGSLTITSHH273 pKa = 5.77 TGNTIVGTFSYY284 pKa = 10.47 SANNGSQTVNISNGSFSITHH304 pKa = 5.84 NN305 pKa = 3.55
Molecular weight: 32.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.862
IPC2_protein 4.126
IPC_protein 4.088
Toseland 3.884
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 4.012
Rodwell 3.923
Grimsley 3.795
Solomon 4.075
Lehninger 4.037
Nozaki 4.202
DTASelect 4.444
Thurlkill 3.935
EMBOSS 4.024
Sillero 4.215
Patrickios 1.964
IPC_peptide 4.075
IPC2_peptide 4.19
IPC2.peptide.svr19 4.107
Protein with the highest isoelectric point:
>tr|A0A1H9CUV5|A0A1H9CUV5_9FLAO Coproporphyrinogen-III oxidase OS=Flavobacterium urocaniciphilum OX=1299341 GN=SAMN05444005_10595 PE=3 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.35 KK16 pKa = 10.31 RR17 pKa = 11.84 KK18 pKa = 9.82 KK19 pKa = 8.65 RR20 pKa = 11.84 ARR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.91 KK27 pKa = 10.59 KK28 pKa = 9.82 KK29 pKa = 10.51 KK30 pKa = 10.04
Molecular weight: 3.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.023
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.705
Grimsley 12.764
Solomon 13.217
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.427
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.989
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2554
0
2554
874636
30
2378
342.5
38.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.184 ± 0.055
0.857 ± 0.023
5.14 ± 0.036
6.472 ± 0.065
5.543 ± 0.047
6.202 ± 0.054
1.621 ± 0.022
8.295 ± 0.054
8.123 ± 0.083
8.809 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.117 ± 0.026
7.115 ± 0.059
3.26 ± 0.032
3.349 ± 0.026
2.809 ± 0.034
6.537 ± 0.049
6.259 ± 0.096
6.212 ± 0.039
0.97 ± 0.016
4.124 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here