Lake Sarah-associated circular virus-33

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GAB3|A0A126GAB3_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-33 OX=1685761 PE=4 SV=1
MM1 pKa = 7.45NNNEE5 pKa = 4.12EE6 pKa = 4.21YY7 pKa = 10.59EE8 pKa = 4.01RR9 pKa = 11.84WLNMDD14 pKa = 3.78TFYY17 pKa = 11.67NPLEE21 pKa = 4.04YY22 pKa = 10.51SQTFTPPLSLTTSQQLPPIPMPMTPIEE49 pKa = 4.31GDD51 pKa = 2.99PRR53 pKa = 11.84EE54 pKa = 4.39RR55 pKa = 11.84VGTTAMCGLPRR66 pKa = 11.84AMCAGAPEE74 pKa = 4.17EE75 pKa = 4.46EE76 pKa = 4.42KK77 pKa = 10.94LASAVCALSLEE88 pKa = 4.35DD89 pKa = 4.6LADD92 pKa = 3.61NKK94 pKa = 9.78EE95 pKa = 4.35TFKK98 pKa = 10.97CRR100 pKa = 11.84SIVFTCYY107 pKa = 9.95KK108 pKa = 10.17RR109 pKa = 11.84EE110 pKa = 4.4AIDD113 pKa = 5.93DD114 pKa = 3.93PEE116 pKa = 5.0AWLRR120 pKa = 11.84GLQSKK125 pKa = 8.03CVWVLGQLEE134 pKa = 4.74KK135 pKa = 10.98CPKK138 pKa = 6.67TQRR141 pKa = 11.84IHH143 pKa = 5.39IQGMAYY149 pKa = 10.11NKK151 pKa = 10.42SDD153 pKa = 3.83IAWAMLKK160 pKa = 10.79GEE162 pKa = 4.5STWKK166 pKa = 9.78RR167 pKa = 11.84KK168 pKa = 9.76CIAPIKK174 pKa = 9.85SIEE177 pKa = 4.22YY178 pKa = 7.21CTKK181 pKa = 10.74AKK183 pKa = 10.46SQLAGPWEE191 pKa = 4.62FGDD194 pKa = 3.81RR195 pKa = 11.84PAYY198 pKa = 9.3GQSAKK203 pKa = 9.34TSKK206 pKa = 10.82LNEE209 pKa = 4.27KK210 pKa = 10.71KK211 pKa = 10.69EE212 pKa = 4.11MNATLLTMTPYY223 pKa = 10.35EE224 pKa = 4.53AVDD227 pKa = 3.12EE228 pKa = 5.52GYY230 pKa = 10.37ISWRR234 pKa = 11.84DD235 pKa = 3.54YY236 pKa = 11.54EE237 pKa = 4.24KK238 pKa = 11.25LKK240 pKa = 11.0LFQTTYY246 pKa = 11.07KK247 pKa = 10.19ADD249 pKa = 3.42KK250 pKa = 10.45KK251 pKa = 10.38IAEE254 pKa = 4.19HH255 pKa = 6.47LKK257 pKa = 10.58RR258 pKa = 11.84PIDD261 pKa = 3.26HH262 pKa = 6.82EE263 pKa = 4.39MRR265 pKa = 11.84HH266 pKa = 5.7EE267 pKa = 4.98WIQGKK272 pKa = 8.8SGKK275 pKa = 10.59GKK277 pKa = 4.37TTKK280 pKa = 10.62AILEE284 pKa = 4.47NPDD287 pKa = 4.19HH288 pKa = 6.24YY289 pKa = 11.49LKK291 pKa = 10.15TINKK295 pKa = 8.65WFDD298 pKa = 3.41DD299 pKa = 3.97YY300 pKa = 11.54QGQEE304 pKa = 4.09TIIIDD309 pKa = 4.14DD310 pKa = 4.9LDD312 pKa = 3.71PTEE315 pKa = 4.35AKK317 pKa = 9.82WMGGNLKK324 pKa = 9.93RR325 pKa = 11.84WADD328 pKa = 3.39KK329 pKa = 9.94WPVPVEE335 pKa = 4.3VKK337 pKa = 10.22HH338 pKa = 4.57GHH340 pKa = 4.95IVIRR344 pKa = 11.84PKK346 pKa = 10.62KK347 pKa = 9.9VVVTTQYY354 pKa = 10.96MMEE357 pKa = 4.31QLWDD361 pKa = 3.94DD362 pKa = 3.77PALIEE367 pKa = 4.1ALKK370 pKa = 10.7RR371 pKa = 11.84RR372 pKa = 11.84FKK374 pKa = 10.78IIDD377 pKa = 3.15VDD379 pKa = 4.91LII381 pKa = 4.03

Molecular weight: 43.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GAB3|A0A126GAB3_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-33 OX=1685761 PE=4 SV=1
MM1 pKa = 7.11VFYY4 pKa = 10.67GRR6 pKa = 11.84TGGLVGLLSRR16 pKa = 11.84GFGGRR21 pKa = 11.84TGFTKK26 pKa = 10.04PGKK29 pKa = 9.87RR30 pKa = 11.84RR31 pKa = 11.84GSFKK35 pKa = 9.85KK36 pKa = 8.26TFRR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 8.39FRR43 pKa = 11.84KK44 pKa = 8.12MRR46 pKa = 11.84YY47 pKa = 8.38RR48 pKa = 11.84KK49 pKa = 9.56KK50 pKa = 10.35RR51 pKa = 11.84YY52 pKa = 7.71NKK54 pKa = 10.14KK55 pKa = 10.34SIGEE59 pKa = 4.01IKK61 pKa = 10.61YY62 pKa = 10.09SPCHH66 pKa = 6.2NGLALTPGQIASTDD80 pKa = 3.49HH81 pKa = 6.79GPATLLQDD89 pKa = 4.07CWPTAGTGVQNVIGRR104 pKa = 11.84QLYY107 pKa = 8.93IRR109 pKa = 11.84KK110 pKa = 9.5LIITLTVANTKK121 pKa = 9.35TNNQQSPMRR130 pKa = 11.84FLLLKK135 pKa = 10.27SKK137 pKa = 10.68KK138 pKa = 9.93FLGGIKK144 pKa = 10.21NNAAFTHH151 pKa = 6.46SSTDD155 pKa = 3.29PAVFPNLFTSTLAYY169 pKa = 9.95SAIPIAQGLGKK180 pKa = 9.14TIFDD184 pKa = 4.29KK185 pKa = 11.23KK186 pKa = 9.35LTPPAAFTTQDD197 pKa = 3.18DD198 pKa = 4.13WVDD201 pKa = 3.16QANYY205 pKa = 8.39VRR207 pKa = 11.84SWKK210 pKa = 8.7KK211 pKa = 8.48TIRR214 pKa = 11.84INKK217 pKa = 9.16VYY219 pKa = 10.36EE220 pKa = 3.5KK221 pKa = 10.48DD222 pKa = 3.19INYY225 pKa = 10.04GSNDD229 pKa = 3.55LFNHH233 pKa = 6.51AGYY236 pKa = 10.55YY237 pKa = 10.13LYY239 pKa = 11.14SLLPTSGQFAGTGAVMNLNYY259 pKa = 10.43YY260 pKa = 8.01FTYY263 pKa = 10.64TDD265 pKa = 3.06VV266 pKa = 3.31

Molecular weight: 29.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

647

266

381

323.5

36.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.801 ± 0.256

1.7 ± 0.592

5.1 ± 0.837

5.1 ± 2.713

3.709 ± 1.438

6.955 ± 1.759

1.7 ± 0.123

6.028 ± 0.477

9.583 ± 0.349

8.192 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.782 ± 0.797

4.328 ± 1.053

5.564 ± 0.657

3.864 ± 0.065

5.1 ± 0.57

4.637 ± 0.625

8.037 ± 1.084

3.864 ± 0.169

2.473 ± 0.839

4.482 ± 0.722
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski