Treponema lecithinolyticum ATCC 700332
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2175 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U2LWK6|U2LWK6_TRELE Uncharacterized protein OS=Treponema lecithinolyticum ATCC 700332 OX=1321815 GN=HMPREF9193_00595 PE=4 SV=1
MM1 pKa = 7.41 QIEE4 pKa = 4.25 KK5 pKa = 10.31 NKK7 pKa = 10.18 RR8 pKa = 11.84 VSIDD12 pKa = 3.33 YY13 pKa = 8.78 TLKK16 pKa = 11.02 DD17 pKa = 3.86 EE18 pKa = 4.69 NGEE21 pKa = 3.98 ILDD24 pKa = 3.8 SSEE27 pKa = 3.77 NGTPLEE33 pKa = 4.16 YY34 pKa = 10.89 VHH36 pKa = 7.11 GYY38 pKa = 10.59 GNLIYY43 pKa = 10.55 GLEE46 pKa = 4.12 KK47 pKa = 10.53 EE48 pKa = 5.14 LEE50 pKa = 4.31 GKK52 pKa = 9.37 QAGDD56 pKa = 3.67 SFSLSVAPAEE66 pKa = 4.49 AYY68 pKa = 10.28 GEE70 pKa = 4.12 YY71 pKa = 10.49 SQDD74 pKa = 4.26 LVVTLPKK81 pKa = 10.95 SNFEE85 pKa = 4.31 SNADD89 pKa = 3.03 ITVGMKK95 pKa = 9.99 FEE97 pKa = 5.06 AGSGLHH103 pKa = 5.76 NRR105 pKa = 11.84 VVTVTKK111 pKa = 10.63 VDD113 pKa = 3.56 GDD115 pKa = 4.39 NITVDD120 pKa = 4.03 ANHH123 pKa = 6.48 EE124 pKa = 4.17 LAGKK128 pKa = 7.7 TLFFDD133 pKa = 3.54 VTVNDD138 pKa = 3.36 VRR140 pKa = 11.84 DD141 pKa = 3.83 ASDD144 pKa = 3.59 EE145 pKa = 4.32 EE146 pKa = 4.25 INEE149 pKa = 4.57 CIGGCSCGSCSSGCGGSDD167 pKa = 3.55 GGCSSGCCGCGFF179 pKa = 4.31
Molecular weight: 18.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.962
IPC2_protein 4.215
IPC_protein 4.164
Toseland 3.973
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.012
Rodwell 3.986
Grimsley 3.884
Solomon 4.113
Lehninger 4.062
Nozaki 4.228
DTASelect 4.406
Thurlkill 3.999
EMBOSS 4.024
Sillero 4.266
Patrickios 0.896
IPC_peptide 4.113
IPC2_peptide 4.253
IPC2.peptide.svr19 4.158
Protein with the highest isoelectric point:
>tr|U2KJQ9|U2KJQ9_TRELE Helicase protein OS=Treponema lecithinolyticum ATCC 700332 OX=1321815 GN=HMPREF9193_01458 PE=4 SV=1
MM1 pKa = 7.59 PPCRR5 pKa = 11.84 HH6 pKa = 5.12 SVGRR10 pKa = 11.84 QAEE13 pKa = 4.08 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 LIINNDD22 pKa = 2.92 CCLRR26 pKa = 11.84 IKK28 pKa = 10.69 SKK30 pKa = 11.24 SLVQKK35 pKa = 10.39 LQFPHH40 pKa = 4.56 QTQYY44 pKa = 11.31 FGG46 pKa = 3.68
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.545
IPC_protein 10.175
Toseland 10.643
ProMoST 10.292
Dawson 10.73
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.994
Grimsley 10.774
Solomon 10.847
Lehninger 10.833
Nozaki 10.687
DTASelect 10.409
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 10.833
IPC_peptide 10.862
IPC2_peptide 9.838
IPC2.peptide.svr19 8.493
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2175
0
2175
736040
39
2136
338.4
37.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.075 ± 0.06
1.503 ± 0.023
5.122 ± 0.041
6.06 ± 0.058
4.971 ± 0.049
6.639 ± 0.054
1.687 ± 0.019
6.766 ± 0.052
7.006 ± 0.051
9.388 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.296 ± 0.026
4.466 ± 0.035
3.667 ± 0.028
3.407 ± 0.032
4.371 ± 0.041
6.589 ± 0.04
5.719 ± 0.04
6.624 ± 0.041
0.981 ± 0.018
3.661 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here