Devosia chinhatensis
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3103 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F5FHB6|A0A0F5FHB6_9RHIZ 3-oxoacyl-ACP synthase OS=Devosia chinhatensis OX=429727 GN=VE26_15395 PE=3 SV=1
MM1 pKa = 7.79 PATGAAIAVLALSMPAMAQEE21 pKa = 4.37 SAAFSLPEE29 pKa = 4.38 LGYY32 pKa = 10.71 DD33 pKa = 3.7 YY34 pKa = 11.37 DD35 pKa = 3.92 ALEE38 pKa = 4.37 PVIDD42 pKa = 4.01 AQTMEE47 pKa = 4.21 LHH49 pKa = 6.35 HH50 pKa = 6.74 SKK52 pKa = 9.53 HH53 pKa = 5.9 HH54 pKa = 6.09 QSFVDD59 pKa = 3.42 NLNNAVEE66 pKa = 5.0 DD67 pKa = 3.89 GTIPADD73 pKa = 3.65 ANVEE77 pKa = 4.09 DD78 pKa = 4.8 LVASASEE85 pKa = 4.42 YY86 pKa = 8.22 PTGIRR91 pKa = 11.84 NSAGGHH97 pKa = 4.73 WNHH100 pKa = 5.88 TFFWQIIGPVEE111 pKa = 4.02 EE112 pKa = 5.2 AGEE115 pKa = 4.19 PSDD118 pKa = 5.62 DD119 pKa = 3.3 FAAAIDD125 pKa = 3.95 AVYY128 pKa = 11.0 GSMDD132 pKa = 3.34 EE133 pKa = 4.22 FKK135 pKa = 11.01 AAFEE139 pKa = 3.96 EE140 pKa = 4.67 AGAGRR145 pKa = 11.84 FGSGWVWLVVTDD157 pKa = 4.3 AGEE160 pKa = 4.18 LEE162 pKa = 4.51 ITTTPNQDD170 pKa = 3.33 NPLMDD175 pKa = 4.15 LAEE178 pKa = 4.29 VQGTPVLGNDD188 pKa = 3.39 VWEE191 pKa = 4.04 HH192 pKa = 6.21 AYY194 pKa = 10.99 YY195 pKa = 9.18 LTYY198 pKa = 10.58 NNRR201 pKa = 11.84 RR202 pKa = 11.84 AEE204 pKa = 4.03 YY205 pKa = 10.34 LSNWWDD211 pKa = 3.37 VVNWDD216 pKa = 4.11 EE217 pKa = 4.87 VSDD220 pKa = 4.66 RR221 pKa = 11.84 YY222 pKa = 10.57 AQALAEE228 pKa = 4.12
Molecular weight: 24.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.923
IPC_protein 3.897
Toseland 3.706
ProMoST 4.012
Dawson 3.872
Bjellqvist 4.05
Wikipedia 3.77
Rodwell 3.732
Grimsley 3.617
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.164
Thurlkill 3.745
EMBOSS 3.783
Sillero 4.012
Patrickios 1.163
IPC_peptide 3.859
IPC2_peptide 3.999
IPC2.peptide.svr19 3.915
Protein with the highest isoelectric point:
>tr|A0A0F5FMZ7|A0A0F5FMZ7_9RHIZ 5-dehydro-2-deoxygluconokinase OS=Devosia chinhatensis OX=429727 GN=VE26_04470 PE=4 SV=1
MM1 pKa = 7.15 VLLFVRR7 pKa = 11.84 RR8 pKa = 11.84 VTFFQTRR15 pKa = 11.84 AIAFRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 AQALQGVLFMWTKK35 pKa = 9.74 PMFRR39 pKa = 11.84 FGLNLLALTAMAVLVLTMAGGISWW63 pKa = 3.66
Molecular weight: 7.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3103
0
3103
967854
41
3899
311.9
33.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.264 ± 0.06
0.656 ± 0.014
5.885 ± 0.039
5.661 ± 0.044
3.857 ± 0.029
8.627 ± 0.048
1.994 ± 0.019
5.56 ± 0.037
3.039 ± 0.039
10.362 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.589 ± 0.023
2.81 ± 0.027
4.962 ± 0.035
3.304 ± 0.026
6.616 ± 0.045
5.451 ± 0.034
5.332 ± 0.036
7.488 ± 0.037
1.3 ± 0.018
2.243 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here