Cucurbit chlorotic yellows virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Crinivirus; unclassified Crinivirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3KY93|D3KY93_9CLOS 59-kDa protein OS=Cucurbit chlorotic yellows virus OX=558690 GN=p59 PE=4 SV=1
MM1 pKa = 7.35GLKK4 pKa = 9.82IVLYY8 pKa = 9.58YY9 pKa = 8.59FNCGFSIYY17 pKa = 10.0YY18 pKa = 10.54CSDD21 pKa = 2.72NRR23 pKa = 11.84LFEE26 pKa = 4.99GDD28 pKa = 3.78FEE30 pKa = 4.94TIYY33 pKa = 11.09TEE35 pKa = 4.2DD36 pKa = 3.84FKK38 pKa = 11.81DD39 pKa = 3.6LLEE42 pKa = 4.37VVTNYY47 pKa = 10.35PFVKK51 pKa = 10.43LQWW54 pKa = 3.8

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3KY91|D3KY91_9CLOS Heat shock 70 kDa-like protein OS=Cucurbit chlorotic yellows virus OX=558690 GN=Hsp70h PE=3 SV=1
MM1 pKa = 7.63PKK3 pKa = 10.2AFKK6 pKa = 10.14FDD8 pKa = 3.61RR9 pKa = 11.84YY10 pKa = 10.34LISLQPMSGSASSKK24 pKa = 9.26NQHH27 pKa = 5.36VSNNIYY33 pKa = 10.47FSFCSIYY40 pKa = 10.82LLSS43 pKa = 3.83

Molecular weight: 4.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

4918

43

2492

447.1

51.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.945 ± 0.515

2.054 ± 0.236

6.446 ± 0.377

5.388 ± 0.319

5.795 ± 0.47

4.921 ± 0.483

2.013 ± 0.226

6.405 ± 0.477

7.889 ± 0.353

9.333 ± 0.625

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.847 ± 0.281

6.669 ± 0.642

3.66 ± 0.438

2.44 ± 0.267

4.758 ± 0.434

7.869 ± 0.551

4.88 ± 0.355

7.32 ± 0.388

0.712 ± 0.108

4.656 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski