Micractinium conductrix

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae; Chlorellales; Chlorellaceae; Chlorella clade; Micractinium

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P6UZ81|A0A2P6UZ81_9CHLO Uncharacterized protein OS=Micractinium conductrix OX=554055 GN=C2E20_9200 PE=4 SV=1
MM1 pKa = 7.53RR2 pKa = 11.84ASPAPLLVLLATLGACGGVAPARR25 pKa = 11.84VLPAEE30 pKa = 4.99DD31 pKa = 4.09PCSSLAALSPATTSAAAIVSGLGLLSGPVPAGLTLFLPSNDD72 pKa = 3.38ALASTTRR79 pKa = 11.84GGAADD84 pKa = 4.06AAQLAALSDD93 pKa = 3.68EE94 pKa = 4.57ARR96 pKa = 11.84GKK98 pKa = 9.98LAAAVLYY105 pKa = 10.88SVGAPAAGALAPDD118 pKa = 4.75ALQSAGQVTTALPDD132 pKa = 3.89APPLAVLADD141 pKa = 4.48DD142 pKa = 4.81GWFADD147 pKa = 4.0AALSLTDD154 pKa = 3.9ATNATAGVAEE164 pKa = 4.41TVQACSSVLYY174 pKa = 10.91VLDD177 pKa = 4.56KK178 pKa = 11.27VLLPAGLLDD187 pKa = 3.69PAFPSVAPDD196 pKa = 3.03QAAAILAAVPTGGTPPLSPAQPLPSPAPASGDD228 pKa = 3.63EE229 pKa = 4.31ARR231 pKa = 11.84DD232 pKa = 3.28AALALLPQLLAAQPTPGFDD251 pKa = 3.91YY252 pKa = 11.43LMLARR257 pKa = 11.84QWGPSFCAAGSCSQPAFNLFTLHH280 pKa = 7.05GLWPNNNDD288 pKa = 3.56SRR290 pKa = 11.84DD291 pKa = 3.83SPASCSGEE299 pKa = 4.25SFDD302 pKa = 5.55EE303 pKa = 4.51AALPPALLTRR313 pKa = 11.84MGCEE317 pKa = 3.58WVSLKK322 pKa = 9.54GTSASFWGYY331 pKa = 8.95EE332 pKa = 3.78WGKK335 pKa = 10.1HH336 pKa = 4.25GTCAVPGLFPNQTAYY351 pKa = 10.43FAAAMALSEE360 pKa = 3.97AWDD363 pKa = 3.54INEE366 pKa = 4.27ALAAAGLDD374 pKa = 3.62PLTARR379 pKa = 11.84SASPNDD385 pKa = 3.45VVAKK389 pKa = 10.09LQAAWGVTPLVTCSDD404 pKa = 3.36GMLQEE409 pKa = 4.08VRR411 pKa = 11.84TCFSTALQPIDD422 pKa = 3.85CPGGSGCATDD432 pKa = 4.16KK433 pKa = 10.85QQEE436 pKa = 4.2LPAGSVDD443 pKa = 3.92PPASCAQVFDD453 pKa = 4.57NVPGLQQ459 pKa = 3.21

Molecular weight: 45.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P6VF86|A0A2P6VF86_9CHLO Nucleotide-diphospho-sugar transferase OS=Micractinium conductrix OX=554055 GN=C2E20_4110 PE=4 SV=1
MM1 pKa = 7.45PSAWQRR7 pKa = 11.84WRR9 pKa = 11.84PWLAAAANLQMRR21 pKa = 11.84AGRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84PGRR29 pKa = 11.84QRR31 pKa = 11.84LNQGHH36 pKa = 5.66QARR39 pKa = 11.84KK40 pKa = 8.97AAGLAGAEE48 pKa = 3.85AAAWATALLNARR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84AGSDD66 pKa = 3.25VKK68 pKa = 10.24WRR70 pKa = 11.84RR71 pKa = 11.84TRR73 pKa = 11.84RR74 pKa = 11.84ASARR78 pKa = 11.84RR79 pKa = 11.84SSPPGPWHH87 pKa = 7.04ANDD90 pKa = 3.35HH91 pKa = 5.99AARR94 pKa = 11.84PAVRR98 pKa = 11.84LRR100 pKa = 11.84RR101 pKa = 11.84AAAGHH106 pKa = 4.93QRR108 pKa = 3.32

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9122

693

9815

6408442

54

7986

652.9

69.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.178 ± 0.054

1.62 ± 0.014

4.451 ± 0.015

5.863 ± 0.027

2.606 ± 0.013

9.29 ± 0.026

2.148 ± 0.009

2.303 ± 0.014

3.041 ± 0.018

9.956 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.869 ± 0.009

1.898 ± 0.011

6.572 ± 0.026

5.35 ± 0.029

6.596 ± 0.019

6.397 ± 0.019

4.376 ± 0.016

6.258 ± 0.015

1.375 ± 0.008

1.854 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski