Capnocytophaga ochracea (strain ATCC 27872 / DSM 7271 / JCM 12966 / NCTC 12371 / VPI 2845) (Bacteroides ochraceus)
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2171 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7M8M1|C7M8M1_CAPOD Peptidyl-prolyl cis-trans isomerase OS=Capnocytophaga ochracea (strain ATCC 27872 / DSM 7271 / JCM 12966 / NCTC 12371 / VPI 2845) OX=521097 GN=Coch_1855 PE=3 SV=1
MM1 pKa = 7.2 VYY3 pKa = 10.24 KK4 pKa = 10.47 FRR6 pKa = 11.84 VILDD10 pKa = 3.34 VKK12 pKa = 10.59 EE13 pKa = 4.43 DD14 pKa = 3.55 VFRR17 pKa = 11.84 DD18 pKa = 3.78 IIIQGEE24 pKa = 4.14 ATLEE28 pKa = 4.12 DD29 pKa = 3.84 LHH31 pKa = 8.34 NVINQSFGFAGDD43 pKa = 3.71 EE44 pKa = 3.98 MASFYY49 pKa = 10.78 LTDD52 pKa = 3.85 EE53 pKa = 4.61 DD54 pKa = 4.31 WNQGEE59 pKa = 5.09 EE60 pKa = 3.72 ITLFDD65 pKa = 3.66 VSEE68 pKa = 4.36 SGEE71 pKa = 3.95 MRR73 pKa = 11.84 LMEE76 pKa = 4.42 EE77 pKa = 4.02 TTIEE81 pKa = 4.31 SVVSEE86 pKa = 4.69 EE87 pKa = 4.2 EE88 pKa = 3.86 PKK90 pKa = 10.83 LLYY93 pKa = 10.78 VYY95 pKa = 10.74 DD96 pKa = 4.91 FMSMWTFFVEE106 pKa = 4.1 LVEE109 pKa = 4.55 IDD111 pKa = 3.72 EE112 pKa = 4.76 EE113 pKa = 4.57 EE114 pKa = 4.29 TGVSYY119 pKa = 9.66 PALIYY124 pKa = 10.79 AHH126 pKa = 6.73 GSIPAEE132 pKa = 4.05 APEE135 pKa = 4.23 KK136 pKa = 11.14 SFVADD141 pKa = 4.29 DD142 pKa = 3.58 FDD144 pKa = 4.18 EE145 pKa = 5.95 FEE147 pKa = 6.04 DD148 pKa = 4.03 EE149 pKa = 5.96 FNDD152 pKa = 4.28 FDD154 pKa = 6.35 LDD156 pKa = 3.79 EE157 pKa = 6.22 DD158 pKa = 4.84 DD159 pKa = 4.65 YY160 pKa = 11.96 GSFSGEE166 pKa = 3.43 EE167 pKa = 4.49 YY168 pKa = 10.79 YY169 pKa = 11.25
Molecular weight: 19.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.801
IPC2_protein 3.541
IPC_protein 3.528
Toseland 3.325
ProMoST 3.681
Dawson 3.503
Bjellqvist 3.656
Wikipedia 3.414
Rodwell 3.363
Grimsley 3.236
Solomon 3.49
Lehninger 3.439
Nozaki 3.617
DTASelect 3.783
Thurlkill 3.376
EMBOSS 3.427
Sillero 3.643
Patrickios 0.985
IPC_peptide 3.49
IPC2_peptide 3.63
IPC2.peptide.svr19 3.644
Protein with the highest isoelectric point:
>tr|C7M5P0|C7M5P0_CAPOD Uncharacterized protein OS=Capnocytophaga ochracea (strain ATCC 27872 / DSM 7271 / JCM 12966 / NCTC 12371 / VPI 2845) OX=521097 GN=Coch_0287 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.19 RR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.93 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 8.3 KK41 pKa = 9.83 LTVSSEE47 pKa = 3.9 PRR49 pKa = 11.84 HH50 pKa = 5.77 KK51 pKa = 10.61 KK52 pKa = 9.84
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2171
0
2171
749313
31
5298
345.1
39.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.969 ± 0.051
0.813 ± 0.023
4.999 ± 0.033
6.632 ± 0.056
4.93 ± 0.049
6.367 ± 0.052
1.881 ± 0.028
7.165 ± 0.043
7.611 ± 0.062
9.337 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.15 ± 0.028
5.523 ± 0.048
3.702 ± 0.035
3.867 ± 0.038
3.888 ± 0.032
5.804 ± 0.033
6.305 ± 0.11
6.509 ± 0.047
1.086 ± 0.018
4.463 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here