Ramie mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato leaf curl Hsinchu virus

Average proteome isoelectric point is 8.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2LYR0|B2LYR0_9GEMI Movement protein BC1 OS=Ramie mosaic virus OX=486809 GN=BC1 PE=3 SV=1
MM1 pKa = 7.94ADD3 pKa = 3.34NSSALAYY10 pKa = 7.52ATSDD14 pKa = 2.93RR15 pKa = 11.84TEE17 pKa = 4.01FQLSSEE23 pKa = 4.16KK24 pKa = 10.54TEE26 pKa = 4.02VTLLFPSTIEE36 pKa = 3.93QKK38 pKa = 10.62LSKK41 pKa = 10.86LMGKK45 pKa = 8.36CLRR48 pKa = 11.84IDD50 pKa = 3.53HH51 pKa = 6.34VVLEE55 pKa = 4.46YY56 pKa = 10.9RR57 pKa = 11.84NQVPVNATGHH67 pKa = 5.87VIIEE71 pKa = 4.03MHH73 pKa = 6.46DD74 pKa = 3.2TRR76 pKa = 11.84LYY78 pKa = 11.08EE79 pKa = 4.53GDD81 pKa = 3.56SKK83 pKa = 11.03QAEE86 pKa = 4.06FTIPIGCNCNIHH98 pKa = 6.3YY99 pKa = 10.23YY100 pKa = 9.48SSSYY104 pKa = 10.42FSPRR108 pKa = 11.84DD109 pKa = 3.52PNPWKK114 pKa = 10.67VMYY117 pKa = 10.31RR118 pKa = 11.84VDD120 pKa = 3.58DD121 pKa = 4.13TNVVDD126 pKa = 5.22GVHH129 pKa = 6.53FCRR132 pKa = 11.84MQGKK136 pKa = 9.91LKK138 pKa = 10.2MSSAKK143 pKa = 9.73QSSEE147 pKa = 3.7IQFKK151 pKa = 10.45SPKK154 pKa = 9.79IEE156 pKa = 4.1ILSKK160 pKa = 10.48AYY162 pKa = 11.2NMTNIDD168 pKa = 4.04FWSVPHH174 pKa = 5.97SQVSRR179 pKa = 11.84RR180 pKa = 11.84PVQGLKK186 pKa = 10.33NLRR189 pKa = 11.84SQSSRR194 pKa = 11.84YY195 pKa = 4.8TTPAILPGQSWASASAVGNEE215 pKa = 4.09EE216 pKa = 4.99QEE218 pKa = 4.12DD219 pKa = 4.04LPYY222 pKa = 11.45GNLHH226 pKa = 6.76RR227 pKa = 11.84LQDD230 pKa = 3.62RR231 pKa = 11.84TLDD234 pKa = 4.04PGPSASEE241 pKa = 4.27VICNTNTVNDD251 pKa = 4.01DD252 pKa = 3.73VINIIKK258 pKa = 8.83KK259 pKa = 7.36TVEE262 pKa = 3.62LCMEE266 pKa = 4.87GSNVTSNVKK275 pKa = 9.77PIKK278 pKa = 10.45

Molecular weight: 31.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2LYQ5|B2LYQ5_9GEMI Replication enhancer OS=Ramie mosaic virus OX=486809 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.23EE17 pKa = 3.68RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPGISRR30 pKa = 11.84AAAPTVLVTNRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84AWTYY47 pKa = 9.4RR48 pKa = 11.84PMYY51 pKa = 9.38RR52 pKa = 11.84KK53 pKa = 9.31PRR55 pKa = 11.84MYY57 pKa = 11.01RR58 pKa = 11.84MFKK61 pKa = 10.72SPDD64 pKa = 3.23VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 9.98VQSFEE79 pKa = 3.95ARR81 pKa = 11.84HH82 pKa = 5.85DD83 pKa = 3.66VAHH86 pKa = 6.38TGKK89 pKa = 10.41VICISDD95 pKa = 3.56VTRR98 pKa = 11.84GGGLTHH104 pKa = 6.43RR105 pKa = 11.84TGKK108 pKa = 9.93RR109 pKa = 11.84FCVKK113 pKa = 9.92SVYY116 pKa = 10.52VLGKK120 pKa = 9.41IWMDD124 pKa = 3.3EE125 pKa = 4.0NIKK128 pKa = 10.41VKK130 pKa = 10.64NHH132 pKa = 5.76TNTVMFFLVRR142 pKa = 11.84DD143 pKa = 3.73RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 9.24GTPQDD152 pKa = 3.82FGQVFNMYY160 pKa = 10.66DD161 pKa = 3.86NEE163 pKa = 4.34PSTATVKK170 pKa = 10.85NDD172 pKa = 2.82LRR174 pKa = 11.84DD175 pKa = 3.33RR176 pKa = 11.84FQVIRR181 pKa = 11.84RR182 pKa = 11.84FTSTVTGGQYY192 pKa = 10.82ASKK195 pKa = 10.24EE196 pKa = 3.7QALVKK201 pKa = 10.45KK202 pKa = 9.7YY203 pKa = 10.09MKK205 pKa = 10.73VNAQVVYY212 pKa = 10.18NHH214 pKa = 5.91QEE216 pKa = 3.56GGKK219 pKa = 9.95YY220 pKa = 9.64EE221 pKa = 4.09NHH223 pKa = 6.46TEE225 pKa = 3.99NALLLYY231 pKa = 7.31MACTHH236 pKa = 6.52SSNPVYY242 pKa = 9.99ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.67FYY252 pKa = 11.26DD253 pKa = 3.51SVSNN257 pKa = 3.9

Molecular weight: 29.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1637

67

359

204.6

23.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.437 ± 0.544

2.321 ± 0.305

5.376 ± 0.428

4.337 ± 0.531

4.337 ± 0.48

5.131 ± 0.515

2.993 ± 0.295

5.376 ± 0.649

5.803 ± 0.485

6.964 ± 0.638

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.955 ± 0.428

5.742 ± 0.602

5.987 ± 0.527

4.52 ± 0.46

7.392 ± 1.07

8.002 ± 0.903

5.864 ± 0.539

6.659 ± 0.808

1.283 ± 0.238

4.52 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski