Leucobacter massiliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2663 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S9QRA9|A0A2S9QRA9_9MICO NADP-dependent oxidoreductase OS=Leucobacter massiliensis OX=1686285 GN=B4915_03425 PE=4 SV=1
MM1 pKa = 7.15HH2 pKa = 6.85QRR4 pKa = 11.84TLTGLLALGAAAALALTGCSNSGDD28 pKa = 3.69ANGEE32 pKa = 3.93NGEE35 pKa = 4.17NGGDD39 pKa = 3.44KK40 pKa = 10.57GTVTLGYY47 pKa = 10.41LPAWTDD53 pKa = 3.82GLSTAYY59 pKa = 10.75LLEE62 pKa = 4.58DD63 pKa = 3.36QLGKK67 pKa = 9.7MGYY70 pKa = 5.46TVEE73 pKa = 4.12MEE75 pKa = 4.41EE76 pKa = 4.05LTEE79 pKa = 4.94AGVLYY84 pKa = 10.76AGLAGGDD91 pKa = 3.45FDD93 pKa = 5.43IYY95 pKa = 10.33PSAWPEE101 pKa = 3.97VTHH104 pKa = 6.93ADD106 pKa = 3.51YY107 pKa = 9.77MKK109 pKa = 10.73QFGDD113 pKa = 3.89SIEE116 pKa = 4.63DD117 pKa = 3.24LGGYY121 pKa = 9.64YY122 pKa = 10.5DD123 pKa = 4.27GAVLTLAVPEE133 pKa = 4.18YY134 pKa = 11.2VDD136 pKa = 3.38IDD138 pKa = 4.36SIEE141 pKa = 4.46DD142 pKa = 3.42LAGAADD148 pKa = 4.23RR149 pKa = 11.84FDD151 pKa = 3.6GQIIGIEE158 pKa = 4.01PGAGLTRR165 pKa = 11.84QTQEE169 pKa = 3.41VAMPEE174 pKa = 4.02YY175 pKa = 10.86GLDD178 pKa = 3.44GDD180 pKa = 4.54YY181 pKa = 11.57EE182 pKa = 4.59LVTSSTATMLATLQDD197 pKa = 4.01KK198 pKa = 10.32IDD200 pKa = 3.87RR201 pKa = 11.84EE202 pKa = 4.05EE203 pKa = 4.34DD204 pKa = 3.71VVVTLWRR211 pKa = 11.84PFWANDD217 pKa = 3.31AFPLKK222 pKa = 10.28DD223 pKa = 3.54LKK225 pKa = 11.07DD226 pKa = 3.42PRR228 pKa = 11.84GAMGEE233 pKa = 4.13PEE235 pKa = 4.38TLHH238 pKa = 6.77FLAKK242 pKa = 10.2QGFSDD247 pKa = 4.02EE248 pKa = 4.25MPEE251 pKa = 3.89VAEE254 pKa = 4.48YY255 pKa = 10.15IAQIKK260 pKa = 10.36LDD262 pKa = 3.96DD263 pKa = 3.92EE264 pKa = 5.38AYY266 pKa = 10.05GALEE270 pKa = 3.92SLVTGDD276 pKa = 4.64EE277 pKa = 4.42YY278 pKa = 11.78ANDD281 pKa = 3.67PQGAVDD287 pKa = 4.32AWLKK291 pKa = 10.56EE292 pKa = 4.05YY293 pKa = 11.22GDD295 pKa = 4.83AYY297 pKa = 10.04PGLITDD303 pKa = 4.4

Molecular weight: 32.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S9QS19|A0A2S9QS19_9MICO Succinate-semialdehyde dehydrogenase OS=Leucobacter massiliensis OX=1686285 GN=B4915_01530 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.5VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.45GRR40 pKa = 11.84SKK42 pKa = 10.13LTAA45 pKa = 4.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2663

0

2663

899312

27

3197

337.7

36.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.09 ± 0.078

0.54 ± 0.01

5.44 ± 0.054

6.663 ± 0.057

3.07 ± 0.031

9.499 ± 0.053

1.917 ± 0.022

4.252 ± 0.035

1.809 ± 0.035

10.347 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.701 ± 0.018

1.805 ± 0.025

5.617 ± 0.035

2.85 ± 0.027

7.894 ± 0.056

5.573 ± 0.048

5.422 ± 0.049

8.283 ± 0.043

1.371 ± 0.019

1.858 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski