Acidithiobacillus sulfuriphilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales; Acidithiobacillaceae; Acidithiobacillus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2736 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M8QXW1|A0A3M8QXW1_9PROT Sigma-54-dependent Fis family transcriptional regulator OS=Acidithiobacillus sulfuriphilus OX=1867749 GN=EC580_09125 PE=4 SV=1
MM1 pKa = 7.6SGISGVVNNALTGLLTAQNALQVTGDD27 pKa = 3.65NIANVNTPGYY37 pKa = 8.3SQQSIVQSTISPTFLGGQFLGNGTQVEE64 pKa = 4.89TVQRR68 pKa = 11.84AYY70 pKa = 11.27SSFLQGQVWSATAAASGQASLSSGLQQVLGLLNGSGGVSSQVSQFFSGVAQVAASPADD128 pKa = 3.33IPARR132 pKa = 11.84QAMLANAQTLAQTFQVLGGQLAGIATGVNQQIGQSVSQINTLTQQIAKK180 pKa = 9.96VNTQIASLQGLGNGSPNDD198 pKa = 3.97LLDD201 pKa = 4.53QRR203 pKa = 11.84DD204 pKa = 3.71QLITQLNQQVGVNVLQQSNGQTNVYY229 pKa = 9.84LSNGQVLVAGSQAFQLQAQASPYY252 pKa = 10.04NGQTMDD258 pKa = 3.27VGYY261 pKa = 10.56ASNGSDD267 pKa = 2.37ITANITGGTLGGLIQFRR284 pKa = 11.84NQSLNPAQNALGQMADD300 pKa = 3.52AMAATLNAQQSQGLDD315 pKa = 3.35LNGQAGKK322 pKa = 10.51ALFASGPVQIFASQFNSGTATISGSISNANQLTSSDD358 pKa = 4.36YY359 pKa = 11.29LLQDD363 pKa = 3.59NGGTWTVTNRR373 pKa = 11.84STGQALAYY381 pKa = 9.51TSGSVGNGTFTSGVAGSVTTLAFAGVQVSVSGANSGDD418 pKa = 3.71SFLVEE423 pKa = 4.21PTRR426 pKa = 11.84LGATAMQAVLGNPSGIAAASPYY448 pKa = 10.13VSNAGILSSGAIVSSNLGNLTLSAGQYY475 pKa = 9.2VSSSGSSDD483 pKa = 3.39VLASGAAFLAPLQITLTSGGSGSASVGFQVSSGGTGSSGIIATGTLSLGGSGTNIDD539 pKa = 3.84IPYY542 pKa = 10.24ASNPPGGYY550 pKa = 8.16WQVNLSGTLAASGDD564 pKa = 4.08AFTLTQAGPGNNGNAAAMAALATSGTMSNGTATFNDD600 pKa = 3.54AAAQLVTQVATQAGQAQTNAAGQQAVLQQATAAQQSLSGVNLDD643 pKa = 3.77QEE645 pKa = 4.56AANLIQYY652 pKa = 7.44QQAYY656 pKa = 7.93QAAAKK661 pKa = 9.77AIQVGNSLFQSLIQALL677 pKa = 3.73

Molecular weight: 67.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M8QNA3|A0A3M8QNA3_9PROT GNAT family N-acetyltransferase OS=Acidithiobacillus sulfuriphilus OX=1867749 GN=EC580_14050 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSIIHH11 pKa = 6.85RR12 pKa = 11.84KK13 pKa = 6.58RR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84SGRR28 pKa = 11.84LVLKK32 pKa = 10.34RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84HH40 pKa = 5.26RR41 pKa = 11.84LSAA44 pKa = 3.8

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2736

0

2736

820862

28

1764

300.0

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.039 ± 0.065

0.932 ± 0.017

4.957 ± 0.035

5.506 ± 0.048

3.536 ± 0.03

8.524 ± 0.046

2.551 ± 0.023

4.955 ± 0.04

2.763 ± 0.039

11.498 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.026

2.623 ± 0.037

5.471 ± 0.037

4.295 ± 0.035

7.188 ± 0.051

5.057 ± 0.035

4.606 ± 0.03

6.942 ± 0.04

1.57 ± 0.026

2.556 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski