Melghiribacillus thermohalophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Melghiribacillus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3015 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3N8I4|A0A4R3N8I4_9BACI ATP phosphoribosyltransferase OS=Melghiribacillus thermohalophilus OX=1324956 GN=hisG PE=3 SV=1
MM1 pKa = 7.79PKK3 pKa = 9.34FTIVDD8 pKa = 3.68KK9 pKa = 8.16EE10 pKa = 4.36TCIACGACGAAAPDD24 pKa = 3.53IYY26 pKa = 10.99DD27 pKa = 3.8YY28 pKa = 11.65DD29 pKa = 4.97DD30 pKa = 5.39DD31 pKa = 6.52GIAFNMLDD39 pKa = 4.45DD40 pKa = 4.01NTGTAEE46 pKa = 3.99IPEE49 pKa = 4.31EE50 pKa = 3.81LHH52 pKa = 6.62EE53 pKa = 5.08DD54 pKa = 3.28MMDD57 pKa = 3.45AFEE60 pKa = 4.82GCPTDD65 pKa = 3.86SIRR68 pKa = 11.84VSAQSFDD75 pKa = 3.42GDD77 pKa = 4.03PNKK80 pKa = 10.38FEE82 pKa = 4.17

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3NC23|A0A4R3NC23_9BACI Uncharacterized protein OS=Melghiribacillus thermohalophilus OX=1324956 GN=EDD68_10279 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.74GRR39 pKa = 11.84KK40 pKa = 9.02VISAA44 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3015

0

3015

846811

29

3027

280.9

31.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.549 ± 0.044

0.638 ± 0.013

5.386 ± 0.031

7.82 ± 0.052

4.555 ± 0.043

6.855 ± 0.047

2.441 ± 0.025

7.682 ± 0.044

6.694 ± 0.049

9.586 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.948 ± 0.025

4.028 ± 0.029

3.812 ± 0.028

4.083 ± 0.032

4.572 ± 0.033

5.626 ± 0.035

5.151 ± 0.034

6.852 ± 0.038

1.051 ± 0.018

3.672 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski