Microbacterium sp. RG1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8KMP2|A0A4P8KMP2_9MICO Phosphate transport system permease protein PstA OS=Microbacterium sp. RG1 OX=2489212 GN=pstA PE=3 SV=1
MM1 pKa = 7.79GIGIGAALAVTAGIAPAAAVDD22 pKa = 3.76PADD25 pKa = 3.64LEE27 pKa = 4.27EE28 pKa = 4.73RR29 pKa = 11.84VTVDD33 pKa = 3.08AVVGHH38 pKa = 6.58LQQLQDD44 pKa = 3.25IADD47 pKa = 3.81ANGGNRR53 pKa = 11.84AAGTSGYY60 pKa = 8.59EE61 pKa = 3.51ASAAYY66 pKa = 9.5IEE68 pKa = 4.35QALTAAGYY76 pKa = 8.21TPEE79 pKa = 4.16RR80 pKa = 11.84QYY82 pKa = 11.8FDD84 pKa = 5.51ADD86 pKa = 3.71LQTIDD91 pKa = 5.21AYY93 pKa = 9.93TLTVDD98 pKa = 4.81GYY100 pKa = 9.48TPADD104 pKa = 3.53PFGIPMEE111 pKa = 4.36FTPGTPAGGLTDD123 pKa = 4.91LPLIAPTAPLGCTGDD138 pKa = 3.65DD139 pKa = 3.43WAGTDD144 pKa = 3.34AAGAIALVSRR154 pKa = 11.84GSCSFAEE161 pKa = 4.44KK162 pKa = 10.24SAAAAAAGAEE172 pKa = 4.04AVIIYY177 pKa = 10.37NNEE180 pKa = 3.81AGPLQGTLGAQTPDD194 pKa = 3.19LAPTVGILQSEE205 pKa = 4.6GQDD208 pKa = 3.53LLAALADD215 pKa = 4.18GPVLADD221 pKa = 5.12LDD223 pKa = 4.25LQQHH227 pKa = 6.18IDD229 pKa = 3.4SVEE232 pKa = 4.03TFNIIADD239 pKa = 4.14TPTGRR244 pKa = 11.84DD245 pKa = 3.6DD246 pKa = 3.81NVVMLGAHH254 pKa = 6.98LDD256 pKa = 3.89SVEE259 pKa = 4.9DD260 pKa = 4.19GPGINDD266 pKa = 3.61NGSGSAAILEE276 pKa = 4.51VALQLAASGEE286 pKa = 4.3LEE288 pKa = 4.17NKK290 pKa = 9.21VRR292 pKa = 11.84FAWWGAEE299 pKa = 3.98EE300 pKa = 4.3IGLLGSAHH308 pKa = 6.15YY309 pKa = 10.78VDD311 pKa = 4.96EE312 pKa = 5.64LSDD315 pKa = 3.72ADD317 pKa = 3.75ADD319 pKa = 5.13AIATYY324 pKa = 11.15LNFDD328 pKa = 3.78MVASPNYY335 pKa = 9.76VIAVYY340 pKa = 10.45DD341 pKa = 4.08ADD343 pKa = 3.49QSTYY347 pKa = 9.03EE348 pKa = 4.34APVEE352 pKa = 4.16VPEE355 pKa = 4.7GSIATEE361 pKa = 3.9AAFTSYY367 pKa = 10.44FDD369 pKa = 6.05AIGQPWIDD377 pKa = 3.35TAFDD381 pKa = 3.23ARR383 pKa = 11.84SDD385 pKa = 3.73YY386 pKa = 11.37NAFILAGIPASGLFTGADD404 pKa = 3.36DD405 pKa = 4.08VKK407 pKa = 10.25TAEE410 pKa = 4.07QVALFGGTEE419 pKa = 4.43GIIMDD424 pKa = 4.89PNYY427 pKa = 8.05HH428 pKa = 6.05TPADD432 pKa = 3.87DD433 pKa = 3.39MSNINTEE440 pKa = 3.95ALGIMLGAMASVTAALANDD459 pKa = 4.1TSAVNGVAPIVDD471 pKa = 4.3PVAPAPEE478 pKa = 4.11VTEE481 pKa = 4.54EE482 pKa = 4.3GDD484 pKa = 3.59HH485 pKa = 6.81LLAASGSEE493 pKa = 4.08ISGVWLGGGVLALLAGAAVLAAAARR518 pKa = 11.84RR519 pKa = 11.84RR520 pKa = 11.84RR521 pKa = 11.84TVV523 pKa = 2.49

Molecular weight: 53.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1EQD3|A0A4V1EQD3_9MICO Nucleotidyltransferase family protein OS=Microbacterium sp. RG1 OX=2489212 GN=EHF32_11790 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3028

0

3028

979390

29

2287

323.4

34.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.147 ± 0.064

0.471 ± 0.01

6.212 ± 0.045

5.493 ± 0.045

3.094 ± 0.028

8.832 ± 0.041

1.893 ± 0.022

4.474 ± 0.03

1.726 ± 0.03

10.173 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.751 ± 0.019

1.846 ± 0.025

5.349 ± 0.03

2.746 ± 0.023

7.556 ± 0.058

5.614 ± 0.028

6.012 ± 0.043

9.124 ± 0.042

1.531 ± 0.019

1.956 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski