Amino acid dipepetide frequency for Microbacterium sp. RG1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.692AlaAla: 22.692 ± 0.292
0.645AlaCys: 0.645 ± 0.027
8.703AlaAsp: 8.703 ± 0.115
8.187AlaGlu: 8.187 ± 0.114
4.072AlaPhe: 4.072 ± 0.07
12.599AlaGly: 12.599 ± 0.131
2.522AlaHis: 2.522 ± 0.056
5.81AlaIle: 5.81 ± 0.085
2.328AlaLys: 2.328 ± 0.052
15.333AlaLeu: 15.333 ± 0.158
2.791AlaMet: 2.791 ± 0.057
2.124AlaAsn: 2.124 ± 0.05
6.944AlaPro: 6.944 ± 0.123
4.135AlaGln: 4.135 ± 0.072
10.04AlaArg: 10.04 ± 0.13
8.072AlaSer: 8.072 ± 0.102
7.566AlaThr: 7.566 ± 0.093
12.364AlaVal: 12.364 ± 0.145
1.99AlaTrp: 1.99 ± 0.046
2.55AlaTyr: 2.55 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.738CysAla: 0.738 ± 0.029
0.037CysCys: 0.037 ± 0.006
0.293CysAsp: 0.293 ± 0.019
0.247CysGlu: 0.247 ± 0.018
0.163CysPhe: 0.163 ± 0.013
0.525CysGly: 0.525 ± 0.026
0.105CysHis: 0.105 ± 0.011
0.159CysIle: 0.159 ± 0.013
0.055CysLys: 0.055 ± 0.009
0.381CysLeu: 0.381 ± 0.022
0.066CysMet: 0.066 ± 0.008
0.086CysAsn: 0.086 ± 0.009
0.259CysPro: 0.259 ± 0.018
0.109CysGln: 0.109 ± 0.011
0.336CysArg: 0.336 ± 0.02
0.288CysSer: 0.288 ± 0.016
0.291CysThr: 0.291 ± 0.017
0.415CysVal: 0.415 ± 0.022
0.067CysTrp: 0.067 ± 0.008
0.092CysTyr: 0.092 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
10.564AspAla: 10.564 ± 0.108
0.21AspCys: 0.21 ± 0.014
4.252AspAsp: 4.252 ± 0.074
4.419AspGlu: 4.419 ± 0.081
1.587AspPhe: 1.587 ± 0.046
5.934AspGly: 5.934 ± 0.092
1.114AspHis: 1.114 ± 0.039
2.455AspIle: 2.455 ± 0.049
0.943AspLys: 0.943 ± 0.038
5.987AspLeu: 5.987 ± 0.069
0.842AspMet: 0.842 ± 0.031
0.92AspAsn: 0.92 ± 0.032
4.126AspPro: 4.126 ± 0.078
1.505AspGln: 1.505 ± 0.042
4.274AspArg: 4.274 ± 0.066
2.317AspSer: 2.317 ± 0.049
3.082AspThr: 3.082 ± 0.062
5.468AspVal: 5.468 ± 0.084
0.915AspTrp: 0.915 ± 0.034
1.208AspTyr: 1.208 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
6.854GluAla: 6.854 ± 0.1
0.207GluCys: 0.207 ± 0.014
2.7GluAsp: 2.7 ± 0.057
2.874GluGlu: 2.874 ± 0.063
1.579GluPhe: 1.579 ± 0.048
3.972GluGly: 3.972 ± 0.07
1.317GluHis: 1.317 ± 0.038
2.808GluIle: 2.808 ± 0.058
1.249GluLys: 1.249 ± 0.045
6.419GluLeu: 6.419 ± 0.087
0.89GluMet: 0.89 ± 0.031
1.259GluAsn: 1.259 ± 0.037
2.933GluPro: 2.933 ± 0.054
2.154GluGln: 2.154 ± 0.05
5.514GluArg: 5.514 ± 0.092
2.705GluSer: 2.705 ± 0.057
3.034GluThr: 3.034 ± 0.059
4.579GluVal: 4.579 ± 0.076
0.792GluTrp: 0.792 ± 0.029
1.085GluTyr: 1.085 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
4.617PheAla: 4.617 ± 0.078
0.148PheCys: 0.148 ± 0.011
2.314PheAsp: 2.314 ± 0.052
1.627PheGlu: 1.627 ± 0.047
1.126PhePhe: 1.126 ± 0.039
3.325PheGly: 3.325 ± 0.069
0.512PheHis: 0.512 ± 0.025
1.132PheIle: 1.132 ± 0.033
0.386PheLys: 0.386 ± 0.022
2.808PheLeu: 2.808 ± 0.062
0.409PheMet: 0.409 ± 0.024
0.574PheAsn: 0.574 ± 0.03
1.33PhePro: 1.33 ± 0.036
0.743PheGln: 0.743 ± 0.03
1.83PheArg: 1.83 ± 0.05
1.865PheSer: 1.865 ± 0.046
2.143PheThr: 2.143 ± 0.043
2.933PheVal: 2.933 ± 0.061
0.498PheTrp: 0.498 ± 0.023
0.618PheTyr: 0.618 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
11.344GlyAla: 11.344 ± 0.133
0.577GlyCys: 0.577 ± 0.029
5.06GlyAsp: 5.06 ± 0.076
5.024GlyGlu: 5.024 ± 0.078
3.263GlyPhe: 3.263 ± 0.058
7.462GlyGly: 7.462 ± 0.106
1.697GlyHis: 1.697 ± 0.042
4.586GlyIle: 4.586 ± 0.065
1.909GlyLys: 1.909 ± 0.055
8.56GlyLeu: 8.56 ± 0.092
1.918GlyMet: 1.918 ± 0.039
1.631GlyAsn: 1.631 ± 0.048
3.659GlyPro: 3.659 ± 0.062
2.341GlyGln: 2.341 ± 0.058
6.395GlyArg: 6.395 ± 0.084
5.287GlySer: 5.287 ± 0.072
5.522GlyThr: 5.522 ± 0.089
8.134GlyVal: 8.134 ± 0.098
1.704GlyTrp: 1.704 ± 0.045
2.251GlyTyr: 2.251 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.37HisAla: 2.37 ± 0.052
0.095HisCys: 0.095 ± 0.01
1.247HisAsp: 1.247 ± 0.036
1.08HisGlu: 1.08 ± 0.038
0.531HisPhe: 0.531 ± 0.026
1.825HisGly: 1.825 ± 0.046
0.505HisHis: 0.505 ± 0.025
0.743HisIle: 0.743 ± 0.026
0.24HisLys: 0.24 ± 0.016
1.982HisLeu: 1.982 ± 0.044
0.325HisMet: 0.325 ± 0.017
0.349HisAsn: 0.349 ± 0.02
1.47HisPro: 1.47 ± 0.04
0.425HisGln: 0.425 ± 0.019
1.599HisArg: 1.599 ± 0.048
0.878HisSer: 0.878 ± 0.028
0.987HisThr: 0.987 ± 0.03
1.645HisVal: 1.645 ± 0.036
0.27HisTrp: 0.27 ± 0.017
0.359HisTyr: 0.359 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
7.171IleAla: 7.171 ± 0.078
0.213IleCys: 0.213 ± 0.017
3.574IleAsp: 3.574 ± 0.065
2.735IleGlu: 2.735 ± 0.051
1.132IlePhe: 1.132 ± 0.04
4.644IleGly: 4.644 ± 0.078
0.648IleHis: 0.648 ± 0.024
1.634IleIle: 1.634 ± 0.052
0.682IleLys: 0.682 ± 0.034
3.633IleLeu: 3.633 ± 0.067
0.643IleMet: 0.643 ± 0.031
0.865IleAsn: 0.865 ± 0.035
2.325IlePro: 2.325 ± 0.042
0.985IleGln: 0.985 ± 0.04
2.695IleArg: 2.695 ± 0.053
2.33IleSer: 2.33 ± 0.052
2.698IleThr: 2.698 ± 0.056
4.882IleVal: 4.882 ± 0.064
0.527IleTrp: 0.527 ± 0.026
0.723IleTyr: 0.723 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
2.14LysAla: 2.14 ± 0.058
0.048LysCys: 0.048 ± 0.007
0.998LysAsp: 0.998 ± 0.037
0.837LysGlu: 0.837 ± 0.034
0.404LysPhe: 0.404 ± 0.023
1.39LysGly: 1.39 ± 0.043
0.353LysHis: 0.353 ± 0.02
0.789LysIle: 0.789 ± 0.03
0.663LysLys: 0.663 ± 0.035
1.68LysLeu: 1.68 ± 0.046
0.303LysMet: 0.303 ± 0.018
0.476LysAsn: 0.476 ± 0.026
1.02LysPro: 1.02 ± 0.035
0.586LysGln: 0.586 ± 0.027
1.363LysArg: 1.363 ± 0.043
0.937LysSer: 0.937 ± 0.035
1.169LysThr: 1.169 ± 0.036
1.503LysVal: 1.503 ± 0.045
0.196LysTrp: 0.196 ± 0.014
0.408LysTyr: 0.408 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
15.349LeuAla: 15.349 ± 0.152
0.501LeuCys: 0.501 ± 0.023
6.462LeuAsp: 6.462 ± 0.089
4.961LeuGlu: 4.961 ± 0.082
2.951LeuPhe: 2.951 ± 0.067
9.089LeuGly: 9.089 ± 0.1
1.783LeuHis: 1.783 ± 0.041
4.42LeuIle: 4.42 ± 0.081
1.515LeuLys: 1.515 ± 0.047
10.271LeuLeu: 10.271 ± 0.155
1.614LeuMet: 1.614 ± 0.039
1.695LeuAsn: 1.695 ± 0.045
5.597LeuPro: 5.597 ± 0.077
2.478LeuGln: 2.478 ± 0.052
7.799LeuArg: 7.799 ± 0.117
6.084LeuSer: 6.084 ± 0.084
6.445LeuThr: 6.445 ± 0.088
9.711LeuVal: 9.711 ± 0.121
1.353LeuTrp: 1.353 ± 0.04
1.645LeuTyr: 1.645 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
2.027MetAla: 2.027 ± 0.05
0.098MetCys: 0.098 ± 0.011
0.793MetAsp: 0.793 ± 0.032
0.57MetGlu: 0.57 ± 0.029
0.485MetPhe: 0.485 ± 0.023
1.329MetGly: 1.329 ± 0.04
0.285MetHis: 0.285 ± 0.015
0.828MetIle: 0.828 ± 0.035
0.36MetLys: 0.36 ± 0.019
1.833MetLeu: 1.833 ± 0.044
0.321MetMet: 0.321 ± 0.019
0.428MetAsn: 0.428 ± 0.02
1.124MetPro: 1.124 ± 0.032
0.506MetGln: 0.506 ± 0.023
1.562MetArg: 1.562 ± 0.039
1.571MetSer: 1.571 ± 0.045
1.645MetThr: 1.645 ± 0.042
1.251MetVal: 1.251 ± 0.04
0.242MetTrp: 0.242 ± 0.016
0.251MetTyr: 0.251 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
2.459AsnAla: 2.459 ± 0.046
0.091AsnCys: 0.091 ± 0.009
1.139AsnAsp: 1.139 ± 0.037
0.926AsnGlu: 0.926 ± 0.036
0.565AsnPhe: 0.565 ± 0.027
1.8AsnGly: 1.8 ± 0.054
0.321AsnHis: 0.321 ± 0.018
0.786AsnIle: 0.786 ± 0.029
0.341AsnLys: 0.341 ± 0.023
1.822AsnLeu: 1.822 ± 0.041
0.304AsnMet: 0.304 ± 0.02
0.435AsnAsn: 0.435 ± 0.023
1.506AsnPro: 1.506 ± 0.046
0.51AsnGln: 0.51 ± 0.024
1.134AsnArg: 1.134 ± 0.035
0.842AsnSer: 0.842 ± 0.035
1.083AsnThr: 1.083 ± 0.038
1.659AsnVal: 1.659 ± 0.049
0.3AsnTrp: 0.3 ± 0.018
0.433AsnTyr: 0.433 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
7.63ProAla: 7.63 ± 0.113
0.166ProCys: 0.166 ± 0.012
3.869ProAsp: 3.869 ± 0.069
3.609ProGlu: 3.609 ± 0.059
1.729ProPhe: 1.729 ± 0.037
4.766ProGly: 4.766 ± 0.084
1.128ProHis: 1.128 ± 0.038
2.067ProIle: 2.067 ± 0.049
0.829ProLys: 0.829 ± 0.031
5.123ProLeu: 5.123 ± 0.073
0.796ProMet: 0.796 ± 0.031
0.925ProAsn: 0.925 ± 0.031
2.246ProPro: 2.246 ± 0.071
1.533ProGln: 1.533 ± 0.043
3.641ProArg: 3.641 ± 0.065
3.144ProSer: 3.144 ± 0.058
3.318ProThr: 3.318 ± 0.061
5.008ProVal: 5.008 ± 0.081
0.9ProTrp: 0.9 ± 0.03
1.064ProTyr: 1.064 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
3.416GlnAla: 3.416 ± 0.064
0.086GlnCys: 0.086 ± 0.01
1.311GlnAsp: 1.311 ± 0.041
1.252GlnGlu: 1.252 ± 0.038
0.833GlnPhe: 0.833 ± 0.027
2.027GlnGly: 2.027 ± 0.043
0.555GlnHis: 0.555 ± 0.026
1.519GlnIle: 1.519 ± 0.044
0.605GlnLys: 0.605 ± 0.028
3.138GlnLeu: 3.138 ± 0.064
0.514GlnMet: 0.514 ± 0.022
0.695GlnAsn: 0.695 ± 0.031
1.441GlnPro: 1.441 ± 0.042
1.169GlnGln: 1.169 ± 0.041
2.423GlnArg: 2.423 ± 0.055
1.519GlnSer: 1.519 ± 0.035
1.548GlnThr: 1.548 ± 0.038
2.344GlnVal: 2.344 ± 0.045
0.459GlnTrp: 0.459 ± 0.023
0.608GlnTyr: 0.608 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
9.725ArgAla: 9.725 ± 0.147
0.329ArgCys: 0.329 ± 0.018
4.344ArgAsp: 4.344 ± 0.07
4.5ArgGlu: 4.5 ± 0.08
2.464ArgPhe: 2.464 ± 0.057
5.703ArgGly: 5.703 ± 0.079
1.558ArgHis: 1.558 ± 0.044
3.87ArgIle: 3.87 ± 0.063
1.2ArgLys: 1.2 ± 0.034
7.562ArgLeu: 7.562 ± 0.102
1.801ArgMet: 1.801 ± 0.048
1.265ArgAsn: 1.265 ± 0.034
3.71ArgPro: 3.71 ± 0.07
1.999ArgGln: 1.999 ± 0.048
7.503ArgArg: 7.503 ± 0.121
4.229ArgSer: 4.229 ± 0.072
4.455ArgThr: 4.455 ± 0.072
6.54ArgVal: 6.54 ± 0.087
1.258ArgTrp: 1.258 ± 0.034
1.55ArgTyr: 1.55 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
7.592SerAla: 7.592 ± 0.111
0.265SerCys: 0.265 ± 0.019
3.424SerAsp: 3.424 ± 0.064
2.757SerGlu: 2.757 ± 0.051
1.968SerPhe: 1.968 ± 0.047
5.758SerGly: 5.758 ± 0.091
1.059SerHis: 1.059 ± 0.031
2.435SerIle: 2.435 ± 0.052
0.874SerLys: 0.874 ± 0.032
5.591SerLeu: 5.591 ± 0.087
1.054SerMet: 1.054 ± 0.036
0.984SerAsn: 0.984 ± 0.033
3.137SerPro: 3.137 ± 0.064
1.436SerGln: 1.436 ± 0.041
3.888SerArg: 3.888 ± 0.071
3.458SerSer: 3.458 ± 0.07
3.394SerThr: 3.394 ± 0.057
4.906SerVal: 4.906 ± 0.073
1.005SerTrp: 1.005 ± 0.032
1.158SerTyr: 1.158 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
8.008ThrAla: 8.008 ± 0.103
0.286ThrCys: 0.286 ± 0.017
3.669ThrAsp: 3.669 ± 0.067
2.814ThrGlu: 2.814 ± 0.049
1.835ThrPhe: 1.835 ± 0.047
5.746ThrGly: 5.746 ± 0.085
1.169ThrHis: 1.169 ± 0.037
2.854ThrIle: 2.854 ± 0.06
1.042ThrLys: 1.042 ± 0.039
6.152ThrLeu: 6.152 ± 0.092
0.893ThrMet: 0.893 ± 0.03
1.143ThrAsn: 1.143 ± 0.038
3.951ThrPro: 3.951 ± 0.071
1.52ThrGln: 1.52 ± 0.042
3.951ThrArg: 3.951 ± 0.066
3.397ThrSer: 3.397 ± 0.057
3.747ThrThr: 3.747 ± 0.068
5.824ThrVal: 5.824 ± 0.094
0.899ThrTrp: 0.899 ± 0.036
1.222ThrTyr: 1.222 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
12.602ValAla: 12.602 ± 0.134
0.489ValCys: 0.489 ± 0.021
5.817ValAsp: 5.817 ± 0.084
4.706ValGlu: 4.706 ± 0.071
2.998ValPhe: 2.998 ± 0.055
7.297ValGly: 7.297 ± 0.103
1.677ValHis: 1.677 ± 0.045
4.386ValIle: 4.386 ± 0.071
1.463ValLys: 1.463 ± 0.043
9.623ValLeu: 9.623 ± 0.116
1.421ValMet: 1.421 ± 0.036
1.706ValAsn: 1.706 ± 0.049
4.899ValPro: 4.899 ± 0.08
2.196ValGln: 2.196 ± 0.049
6.56ValArg: 6.56 ± 0.09
5.279ValSer: 5.279 ± 0.068
5.929ValThr: 5.929 ± 0.099
9.326ValVal: 9.326 ± 0.131
1.251ValTrp: 1.251 ± 0.036
1.616ValTyr: 1.616 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.665TrpAla: 1.665 ± 0.045
0.095TrpCys: 0.095 ± 0.01
0.809TrpAsp: 0.809 ± 0.029
0.707TrpGlu: 0.707 ± 0.025
0.572TrpPhe: 0.572 ± 0.024
1.183TrpGly: 1.183 ± 0.042
0.332TrpHis: 0.332 ± 0.021
0.744TrpIle: 0.744 ± 0.025
0.261TrpLys: 0.261 ± 0.019
1.779TrpLeu: 1.779 ± 0.046
0.363TrpMet: 0.363 ± 0.019
0.43TrpAsn: 0.43 ± 0.024
0.774TrpPro: 0.774 ± 0.028
0.589TrpGln: 0.589 ± 0.024
1.342TrpArg: 1.342 ± 0.04
0.92TrpSer: 0.92 ± 0.028
0.899TrpThr: 0.899 ± 0.03
1.204TrpVal: 1.204 ± 0.036
0.372TrpTrp: 0.372 ± 0.022
0.273TrpTyr: 0.273 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.502TyrAla: 2.502 ± 0.049
0.126TyrCys: 0.126 ± 0.011
1.346TyrAsp: 1.346 ± 0.035
1.094TyrGlu: 1.094 ± 0.037
0.683TyrPhe: 0.683 ± 0.028
1.95TyrGly: 1.95 ± 0.044
0.285TyrHis: 0.285 ± 0.017
0.713TyrIle: 0.713 ± 0.03
0.317TyrLys: 0.317 ± 0.02
2.05TyrLeu: 2.05 ± 0.051
0.239TyrMet: 0.239 ± 0.016
0.468TyrAsn: 0.468 ± 0.024
1.037TyrPro: 1.037 ± 0.034
0.542TyrGln: 0.542 ± 0.026
1.715TyrArg: 1.715 ± 0.047
1.018TyrSer: 1.018 ± 0.033
1.169TyrThr: 1.169 ± 0.041
1.547TyrVal: 1.547 ± 0.039
0.314TyrTrp: 0.314 ± 0.02
0.443TyrTyr: 0.443 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3028 proteins (979390 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski