Escherichia phage phiV10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Uetakevirus; Escherichia virus phiV10

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q287A5|Q287A5_9CAUD Uncharacterized protein OS=Escherichia phage phiV10 OX=343516 GN=PhiV10p13 PE=4 SV=1
MM1 pKa = 7.45ASVVEE6 pKa = 4.07ICNRR10 pKa = 11.84ALSNIGNSRR19 pKa = 11.84SINSLTEE26 pKa = 3.67ASKK29 pKa = 10.82EE30 pKa = 3.83AGEE33 pKa = 4.44CSLHH37 pKa = 7.48FEE39 pKa = 4.34ACRR42 pKa = 11.84DD43 pKa = 3.65AVLSDD48 pKa = 3.89FDD50 pKa = 4.17WNFATKK56 pKa = 10.02RR57 pKa = 11.84VALADD62 pKa = 3.78TSNPPPDD69 pKa = 3.13WEE71 pKa = 4.13YY72 pKa = 11.31AYY74 pKa = 10.21QYY76 pKa = 10.89PSDD79 pKa = 4.04CLRR82 pKa = 11.84ITEE85 pKa = 4.0IMLPGVRR92 pKa = 11.84NPTAAMRR99 pKa = 11.84VQYY102 pKa = 10.47EE103 pKa = 4.2VGADD107 pKa = 3.49TNGTGKK113 pKa = 10.79LIYY116 pKa = 9.79TDD118 pKa = 5.18QPQAWLKK125 pKa = 9.24YY126 pKa = 7.96VSRR129 pKa = 11.84VTDD132 pKa = 3.15VNMFDD137 pKa = 4.4AIFMEE142 pKa = 4.35ALAWRR147 pKa = 11.84LAAAINMALTGNADD161 pKa = 3.71LGTFALNMYY170 pKa = 10.15NRR172 pKa = 11.84VILSAGSHH180 pKa = 5.44SQNEE184 pKa = 4.48SQEE187 pKa = 4.04PQPPVDD193 pKa = 3.53EE194 pKa = 4.3FTIARR199 pKa = 11.84LSS201 pKa = 3.38

Molecular weight: 22.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q286W7|Q286W7_9CAUD Uncharacterized protein OS=Escherichia phage phiV10 OX=343516 GN=PhiV10p50 PE=4 SV=1
MM1 pKa = 7.39LRR3 pKa = 11.84GDD5 pKa = 4.45GAQLLQKK12 pKa = 9.66AQRR15 pKa = 11.84VTEE18 pKa = 3.98QAEE21 pKa = 3.95EE22 pKa = 4.04RR23 pKa = 11.84VRR25 pKa = 11.84SKK27 pKa = 10.37MKK29 pKa = 10.07NRR31 pKa = 11.84KK32 pKa = 9.11AKK34 pKa = 8.8MLISRR39 pKa = 11.84VYY41 pKa = 9.23RR42 pKa = 11.84RR43 pKa = 11.84CYY45 pKa = 8.07PSQWLRR51 pKa = 11.84VSNRR55 pKa = 11.84RR56 pKa = 11.84VVLYY60 pKa = 9.64LYY62 pKa = 10.71SGIARR67 pKa = 11.84EE68 pKa = 4.36GIKK71 pKa = 10.35DD72 pKa = 3.56KK73 pKa = 11.16RR74 pKa = 11.84SAAQNRR80 pKa = 11.84WKK82 pKa = 10.79NHH84 pKa = 4.22LRR86 pKa = 11.84AKK88 pKa = 10.63GDD90 pKa = 3.32

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12288

29

875

223.4

24.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.075 ± 0.562

1.017 ± 0.132

5.672 ± 0.251

6.03 ± 0.338

3.263 ± 0.208

7.536 ± 0.323

1.497 ± 0.132

5.68 ± 0.296

5.094 ± 0.29

7.992 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.019 ± 0.232

4.704 ± 0.274

4.053 ± 0.207

4.753 ± 0.385

6.234 ± 0.304

6.567 ± 0.347

5.632 ± 0.387

6.364 ± 0.266

1.693 ± 0.152

3.125 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski