Amino acid dipepetide frequency for Escherichia phage phiV10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.127AlaAla: 12.127 ± 1.625
0.814AlaCys: 0.814 ± 0.259
5.697AlaAsp: 5.697 ± 0.755
6.755AlaGlu: 6.755 ± 0.992
3.744AlaPhe: 3.744 ± 0.548
7.732AlaGly: 7.732 ± 0.982
1.221AlaHis: 1.221 ± 0.31
4.069AlaIle: 4.069 ± 0.54
4.883AlaLys: 4.883 ± 0.621
8.22AlaLeu: 8.22 ± 0.882
3.907AlaMet: 3.907 ± 0.551
4.639AlaAsn: 4.639 ± 0.503
3.174AlaPro: 3.174 ± 0.598
5.046AlaGln: 5.046 ± 0.919
5.453AlaArg: 5.453 ± 0.68
6.104AlaSer: 6.104 ± 0.691
5.209AlaThr: 5.209 ± 0.539
6.999AlaVal: 6.999 ± 0.835
1.791AlaTrp: 1.791 ± 0.456
3.174AlaTyr: 3.174 ± 0.538
0.0AlaXaa: 0.0 ± 0.0
Cys
0.651CysAla: 0.651 ± 0.207
0.326CysCys: 0.326 ± 0.204
0.57CysAsp: 0.57 ± 0.214
0.57CysGlu: 0.57 ± 0.183
0.488CysPhe: 0.488 ± 0.187
1.221CysGly: 1.221 ± 0.341
0.081CysHis: 0.081 ± 0.087
0.651CysIle: 0.651 ± 0.17
0.57CysLys: 0.57 ± 0.248
0.895CysLeu: 0.895 ± 0.335
0.407CysMet: 0.407 ± 0.18
0.326CysAsn: 0.326 ± 0.18
0.407CysPro: 0.407 ± 0.165
0.244CysGln: 0.244 ± 0.15
1.058CysArg: 1.058 ± 0.299
0.57CysSer: 0.57 ± 0.195
0.57CysThr: 0.57 ± 0.227
0.244CysVal: 0.244 ± 0.13
0.081CysTrp: 0.081 ± 0.075
0.244CysTyr: 0.244 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
5.941AspAla: 5.941 ± 0.853
0.814AspCys: 0.814 ± 0.272
3.988AspAsp: 3.988 ± 0.571
3.581AspGlu: 3.581 ± 0.685
1.384AspPhe: 1.384 ± 0.33
4.558AspGly: 4.558 ± 0.663
0.407AspHis: 0.407 ± 0.238
3.662AspIle: 3.662 ± 0.422
3.988AspLys: 3.988 ± 0.734
4.151AspLeu: 4.151 ± 0.567
1.628AspMet: 1.628 ± 0.408
2.442AspAsn: 2.442 ± 0.316
2.442AspPro: 2.442 ± 0.573
2.035AspGln: 2.035 ± 0.318
2.604AspArg: 2.604 ± 0.605
3.011AspSer: 3.011 ± 0.349
2.93AspThr: 2.93 ± 0.439
4.395AspVal: 4.395 ± 0.654
1.058AspTrp: 1.058 ± 0.303
1.709AspTyr: 1.709 ± 0.312
0.0AspXaa: 0.0 ± 0.0
Glu
6.185GluAla: 6.185 ± 0.737
0.814GluCys: 0.814 ± 0.23
3.011GluAsp: 3.011 ± 0.571
3.011GluGlu: 3.011 ± 0.592
3.011GluPhe: 3.011 ± 0.479
3.418GluGly: 3.418 ± 0.524
0.732GluHis: 0.732 ± 0.243
3.581GluIle: 3.581 ± 0.476
3.255GluLys: 3.255 ± 0.586
4.883GluLeu: 4.883 ± 0.712
1.465GluMet: 1.465 ± 0.334
2.035GluAsn: 2.035 ± 0.37
2.279GluPro: 2.279 ± 0.356
4.395GluGln: 4.395 ± 0.589
4.151GluArg: 4.151 ± 0.582
4.151GluSer: 4.151 ± 0.623
3.337GluThr: 3.337 ± 0.467
3.337GluVal: 3.337 ± 0.5
0.651GluTrp: 0.651 ± 0.312
2.604GluTyr: 2.604 ± 0.446
0.0GluXaa: 0.0 ± 0.0
Phe
2.767PheAla: 2.767 ± 0.523
0.407PheCys: 0.407 ± 0.177
2.116PheAsp: 2.116 ± 0.433
1.953PheGlu: 1.953 ± 0.351
1.302PhePhe: 1.302 ± 0.312
2.36PheGly: 2.36 ± 0.445
0.244PheHis: 0.244 ± 0.214
2.686PheIle: 2.686 ± 0.635
1.384PheLys: 1.384 ± 0.292
1.546PheLeu: 1.546 ± 0.435
0.977PheMet: 0.977 ± 0.247
1.628PheAsn: 1.628 ± 0.356
1.221PhePro: 1.221 ± 0.365
0.895PheGln: 0.895 ± 0.26
3.011PheArg: 3.011 ± 0.331
2.686PheSer: 2.686 ± 0.501
2.035PheThr: 2.035 ± 0.414
1.791PheVal: 1.791 ± 0.354
0.651PheTrp: 0.651 ± 0.237
0.977PheTyr: 0.977 ± 0.286
0.0PheXaa: 0.0 ± 0.0
Gly
5.534GlyAla: 5.534 ± 1.007
0.895GlyCys: 0.895 ± 0.308
4.314GlyAsp: 4.314 ± 0.636
4.965GlyGlu: 4.965 ± 0.671
3.255GlyPhe: 3.255 ± 0.609
5.86GlyGly: 5.86 ± 0.754
1.465GlyHis: 1.465 ± 0.29
4.558GlyIle: 4.558 ± 0.537
4.802GlyLys: 4.802 ± 0.73
6.185GlyLeu: 6.185 ± 0.839
2.197GlyMet: 2.197 ± 0.401
4.069GlyAsn: 4.069 ± 0.583
1.546GlyPro: 1.546 ± 0.318
3.744GlyGln: 3.744 ± 0.59
3.5GlyArg: 3.5 ± 0.513
3.988GlySer: 3.988 ± 0.656
4.314GlyThr: 4.314 ± 0.573
6.267GlyVal: 6.267 ± 0.887
1.058GlyTrp: 1.058 ± 0.312
2.849GlyTyr: 2.849 ± 0.358
0.0GlyXaa: 0.0 ± 0.0
His
1.058HisAla: 1.058 ± 0.317
0.407HisCys: 0.407 ± 0.215
0.732HisAsp: 0.732 ± 0.215
1.302HisGlu: 1.302 ± 0.351
0.407HisPhe: 0.407 ± 0.163
1.302HisGly: 1.302 ± 0.263
0.407HisHis: 0.407 ± 0.147
0.814HisIle: 0.814 ± 0.191
0.163HisLys: 0.163 ± 0.095
1.302HisLeu: 1.302 ± 0.338
0.407HisMet: 0.407 ± 0.167
0.651HisAsn: 0.651 ± 0.273
1.058HisPro: 1.058 ± 0.398
0.326HisGln: 0.326 ± 0.181
0.814HisArg: 0.814 ± 0.311
1.221HisSer: 1.221 ± 0.316
0.814HisThr: 0.814 ± 0.217
0.977HisVal: 0.977 ± 0.234
0.488HisTrp: 0.488 ± 0.206
0.326HisTyr: 0.326 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
6.348IleAla: 6.348 ± 0.86
0.407IleCys: 0.407 ± 0.21
3.988IleAsp: 3.988 ± 0.698
3.744IleGlu: 3.744 ± 0.555
1.384IlePhe: 1.384 ± 0.281
4.069IleGly: 4.069 ± 0.432
0.651IleHis: 0.651 ± 0.209
3.337IleIle: 3.337 ± 0.605
3.255IleLys: 3.255 ± 0.526
3.174IleLeu: 3.174 ± 0.701
1.465IleMet: 1.465 ± 0.452
3.5IleAsn: 3.5 ± 0.443
2.686IlePro: 2.686 ± 0.416
2.197IleGln: 2.197 ± 0.356
3.418IleArg: 3.418 ± 0.404
4.069IleSer: 4.069 ± 0.576
4.232IleThr: 4.232 ± 0.544
2.686IleVal: 2.686 ± 0.473
0.895IleTrp: 0.895 ± 0.226
1.302IleTyr: 1.302 ± 0.324
0.0IleXaa: 0.0 ± 0.0
Lys
4.639LysAla: 4.639 ± 0.699
0.57LysCys: 0.57 ± 0.231
2.197LysAsp: 2.197 ± 0.466
2.523LysGlu: 2.523 ± 0.484
1.384LysPhe: 1.384 ± 0.336
3.744LysGly: 3.744 ± 0.685
0.814LysHis: 0.814 ± 0.191
2.604LysIle: 2.604 ± 0.47
2.523LysLys: 2.523 ± 0.414
4.232LysLeu: 4.232 ± 0.575
1.384LysMet: 1.384 ± 0.388
2.849LysAsn: 2.849 ± 0.603
3.011LysPro: 3.011 ± 0.557
2.523LysGln: 2.523 ± 0.512
3.825LysArg: 3.825 ± 0.706
2.767LysSer: 2.767 ± 0.453
3.337LysThr: 3.337 ± 0.44
2.93LysVal: 2.93 ± 0.449
1.465LysTrp: 1.465 ± 0.336
1.628LysTyr: 1.628 ± 0.399
0.0LysXaa: 0.0 ± 0.0
Leu
8.627LeuAla: 8.627 ± 0.74
0.651LeuCys: 0.651 ± 0.245
4.314LeuAsp: 4.314 ± 0.371
4.72LeuGlu: 4.72 ± 0.693
2.93LeuPhe: 2.93 ± 0.666
5.046LeuGly: 5.046 ± 0.869
1.546LeuHis: 1.546 ± 0.358
4.72LeuIle: 4.72 ± 0.804
3.5LeuLys: 3.5 ± 0.539
6.674LeuLeu: 6.674 ± 0.834
2.279LeuMet: 2.279 ± 0.406
4.314LeuAsn: 4.314 ± 0.532
2.442LeuPro: 2.442 ± 0.433
3.174LeuGln: 3.174 ± 0.43
5.453LeuArg: 5.453 ± 0.805
6.511LeuSer: 6.511 ± 0.63
4.965LeuThr: 4.965 ± 0.662
4.558LeuVal: 4.558 ± 0.481
0.814LeuTrp: 0.814 ± 0.228
2.686LeuTyr: 2.686 ± 0.42
0.0LeuXaa: 0.0 ± 0.0
Met
4.069MetAla: 4.069 ± 0.912
0.244MetCys: 0.244 ± 0.125
1.139MetAsp: 1.139 ± 0.223
1.465MetGlu: 1.465 ± 0.34
0.977MetPhe: 0.977 ± 0.269
1.872MetGly: 1.872 ± 0.327
0.244MetHis: 0.244 ± 0.139
1.465MetIle: 1.465 ± 0.423
1.709MetLys: 1.709 ± 0.458
3.255MetLeu: 3.255 ± 0.671
1.139MetMet: 1.139 ± 0.394
1.953MetAsn: 1.953 ± 0.425
1.221MetPro: 1.221 ± 0.33
0.57MetGln: 0.57 ± 0.176
1.953MetArg: 1.953 ± 0.607
2.849MetSer: 2.849 ± 0.545
1.791MetThr: 1.791 ± 0.449
1.221MetVal: 1.221 ± 0.227
0.244MetTrp: 0.244 ± 0.146
0.814MetTyr: 0.814 ± 0.285
0.0MetXaa: 0.0 ± 0.0
Asn
4.232AsnAla: 4.232 ± 0.669
0.326AsnCys: 0.326 ± 0.171
2.686AsnAsp: 2.686 ± 0.426
2.604AsnGlu: 2.604 ± 0.38
1.221AsnPhe: 1.221 ± 0.196
4.476AsnGly: 4.476 ± 0.725
0.57AsnHis: 0.57 ± 0.223
2.767AsnIle: 2.767 ± 0.499
2.442AsnLys: 2.442 ± 0.405
3.174AsnLeu: 3.174 ± 0.582
1.302AsnMet: 1.302 ± 0.423
1.953AsnAsn: 1.953 ± 0.507
2.767AsnPro: 2.767 ± 0.545
2.604AsnGln: 2.604 ± 0.485
3.255AsnArg: 3.255 ± 0.532
3.255AsnSer: 3.255 ± 0.786
2.442AsnThr: 2.442 ± 0.427
2.604AsnVal: 2.604 ± 0.478
0.732AsnTrp: 0.732 ± 0.25
1.628AsnTyr: 1.628 ± 0.409
0.0AsnXaa: 0.0 ± 0.0
Pro
3.093ProAla: 3.093 ± 0.614
0.163ProCys: 0.163 ± 0.111
3.337ProAsp: 3.337 ± 0.558
3.255ProGlu: 3.255 ± 0.515
1.139ProPhe: 1.139 ± 0.27
4.151ProGly: 4.151 ± 0.501
0.977ProHis: 0.977 ± 0.275
1.628ProIle: 1.628 ± 0.326
1.709ProLys: 1.709 ± 0.475
2.523ProLeu: 2.523 ± 0.53
0.814ProMet: 0.814 ± 0.218
0.814ProAsn: 0.814 ± 0.223
1.628ProPro: 1.628 ± 0.486
2.279ProGln: 2.279 ± 0.382
1.465ProArg: 1.465 ± 0.3
3.337ProSer: 3.337 ± 0.549
1.953ProThr: 1.953 ± 0.38
4.151ProVal: 4.151 ± 0.546
0.895ProTrp: 0.895 ± 0.276
1.221ProTyr: 1.221 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
6.104GlnAla: 6.104 ± 1.179
0.57GlnCys: 0.57 ± 0.253
1.628GlnAsp: 1.628 ± 0.314
3.093GlnGlu: 3.093 ± 0.387
1.465GlnPhe: 1.465 ± 0.37
2.279GlnGly: 2.279 ± 0.378
0.732GlnHis: 0.732 ± 0.228
2.197GlnIle: 2.197 ± 0.505
1.953GlnLys: 1.953 ± 0.422
5.127GlnLeu: 5.127 ± 0.767
1.872GlnMet: 1.872 ± 0.37
1.546GlnAsn: 1.546 ± 0.294
1.791GlnPro: 1.791 ± 0.401
3.744GlnGln: 3.744 ± 0.841
3.5GlnArg: 3.5 ± 0.646
2.035GlnSer: 2.035 ± 0.5
2.035GlnThr: 2.035 ± 0.473
2.93GlnVal: 2.93 ± 0.628
1.139GlnTrp: 1.139 ± 0.347
1.709GlnTyr: 1.709 ± 0.339
0.0GlnXaa: 0.0 ± 0.0
Arg
6.104ArgAla: 6.104 ± 0.787
0.488ArgCys: 0.488 ± 0.153
3.825ArgAsp: 3.825 ± 0.517
4.232ArgGlu: 4.232 ± 0.604
1.546ArgPhe: 1.546 ± 0.277
3.174ArgGly: 3.174 ± 0.531
1.058ArgHis: 1.058 ± 0.294
4.639ArgIle: 4.639 ± 0.56
4.232ArgLys: 4.232 ± 0.498
5.046ArgLeu: 5.046 ± 0.636
1.221ArgMet: 1.221 ± 0.294
3.093ArgAsn: 3.093 ± 0.485
1.221ArgPro: 1.221 ± 0.273
4.069ArgGln: 4.069 ± 0.57
5.127ArgArg: 5.127 ± 0.718
2.93ArgSer: 2.93 ± 0.584
3.011ArgThr: 3.011 ± 0.53
4.314ArgVal: 4.314 ± 0.867
1.546ArgTrp: 1.546 ± 0.414
1.465ArgTyr: 1.465 ± 0.314
0.0ArgXaa: 0.0 ± 0.0
Ser
6.104SerAla: 6.104 ± 0.727
0.651SerCys: 0.651 ± 0.234
4.069SerAsp: 4.069 ± 0.582
3.825SerGlu: 3.825 ± 0.598
2.197SerPhe: 2.197 ± 0.433
5.941SerGly: 5.941 ± 0.898
0.895SerHis: 0.895 ± 0.248
2.93SerIle: 2.93 ± 0.428
3.174SerLys: 3.174 ± 0.504
5.778SerLeu: 5.778 ± 0.695
2.279SerMet: 2.279 ± 0.459
3.093SerAsn: 3.093 ± 0.552
3.337SerPro: 3.337 ± 0.429
2.767SerGln: 2.767 ± 0.622
4.151SerArg: 4.151 ± 0.594
3.907SerSer: 3.907 ± 0.711
3.744SerThr: 3.744 ± 0.518
4.069SerVal: 4.069 ± 0.618
0.814SerTrp: 0.814 ± 0.173
1.953SerTyr: 1.953 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
6.836ThrAla: 6.836 ± 0.838
0.57ThrCys: 0.57 ± 0.223
3.5ThrAsp: 3.5 ± 0.722
2.36ThrGlu: 2.36 ± 0.459
1.384ThrPhe: 1.384 ± 0.286
7.406ThrGly: 7.406 ± 0.932
0.732ThrHis: 0.732 ± 0.214
3.174ThrIle: 3.174 ± 0.631
2.279ThrLys: 2.279 ± 0.495
4.232ThrLeu: 4.232 ± 0.586
1.709ThrMet: 1.709 ± 0.315
2.279ThrAsn: 2.279 ± 0.379
3.5ThrPro: 3.5 ± 0.461
1.953ThrGln: 1.953 ± 0.514
2.686ThrArg: 2.686 ± 0.528
3.825ThrSer: 3.825 ± 0.802
2.686ThrThr: 2.686 ± 0.452
3.255ThrVal: 3.255 ± 0.68
0.814ThrTrp: 0.814 ± 0.298
1.139ThrTyr: 1.139 ± 0.35
0.0ThrXaa: 0.0 ± 0.0
Val
5.778ValAla: 5.778 ± 0.619
0.488ValCys: 0.488 ± 0.181
3.581ValAsp: 3.581 ± 0.615
3.174ValGlu: 3.174 ± 0.398
1.872ValPhe: 1.872 ± 0.356
2.93ValGly: 2.93 ± 0.604
1.058ValHis: 1.058 ± 0.242
4.232ValIle: 4.232 ± 0.654
3.255ValLys: 3.255 ± 0.505
4.72ValLeu: 4.72 ± 0.582
2.686ValMet: 2.686 ± 0.487
4.151ValAsn: 4.151 ± 0.609
2.442ValPro: 2.442 ± 0.515
2.604ValGln: 2.604 ± 0.4
3.744ValArg: 3.744 ± 0.44
4.883ValSer: 4.883 ± 0.69
4.232ValThr: 4.232 ± 0.565
4.639ValVal: 4.639 ± 0.72
0.895ValTrp: 0.895 ± 0.209
2.279ValTyr: 2.279 ± 0.395
0.0ValXaa: 0.0 ± 0.0
Trp
1.872TrpAla: 1.872 ± 0.361
0.163TrpCys: 0.163 ± 0.121
0.57TrpAsp: 0.57 ± 0.209
1.058TrpGlu: 1.058 ± 0.344
0.651TrpPhe: 0.651 ± 0.202
1.302TrpGly: 1.302 ± 0.379
0.244TrpHis: 0.244 ± 0.136
0.651TrpIle: 0.651 ± 0.243
0.814TrpLys: 0.814 ± 0.281
2.035TrpLeu: 2.035 ± 0.451
0.488TrpMet: 0.488 ± 0.197
0.814TrpAsn: 0.814 ± 0.216
0.407TrpPro: 0.407 ± 0.201
0.57TrpGln: 0.57 ± 0.236
1.139TrpArg: 1.139 ± 0.303
1.465TrpSer: 1.465 ± 0.423
0.732TrpThr: 0.732 ± 0.207
0.977TrpVal: 0.977 ± 0.313
0.326TrpTrp: 0.326 ± 0.154
0.651TrpTyr: 0.651 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.686TyrAla: 2.686 ± 0.379
0.407TyrCys: 0.407 ± 0.159
1.465TyrAsp: 1.465 ± 0.322
2.116TyrGlu: 2.116 ± 0.382
0.895TyrPhe: 0.895 ± 0.277
2.523TyrGly: 2.523 ± 0.524
0.895TyrHis: 0.895 ± 0.305
2.442TyrIle: 2.442 ± 0.388
1.058TyrLys: 1.058 ± 0.244
2.767TyrLeu: 2.767 ± 0.596
0.488TyrMet: 0.488 ± 0.221
0.895TyrAsn: 0.895 ± 0.307
1.953TyrPro: 1.953 ± 0.462
1.791TyrGln: 1.791 ± 0.409
2.035TyrArg: 2.035 ± 0.456
2.116TyrSer: 2.116 ± 0.354
1.953TyrThr: 1.953 ± 0.484
1.302TyrVal: 1.302 ± 0.351
0.57TyrTrp: 0.57 ± 0.209
0.895TyrTyr: 0.895 ± 0.256
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12288 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski