Pestalotiopsis fici (strain W106-1 / CGMCC3.15140)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Sporocadaceae; Pestalotiopsis; Pestalotiopsis fici

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15412 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W3XH92|W3XH92_PESFW Uncharacterized protein OS=Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) OX=1229662 GN=PFICI_03430 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 4.4FADD5 pKa = 4.68VLTCIYY11 pKa = 10.42VQDD14 pKa = 3.7PSYY17 pKa = 11.2FKK19 pKa = 11.17GKK21 pKa = 8.0TPLYY25 pKa = 9.79TGTIDD30 pKa = 5.1DD31 pKa = 5.38LNFSTDD37 pKa = 3.13TDD39 pKa = 3.69GMLLNLGAVPINASQIAVVAQGYY62 pKa = 8.66FRR64 pKa = 11.84AKK66 pKa = 9.1HH67 pKa = 5.11TGSYY71 pKa = 10.4YY72 pKa = 10.02IGSTGFSTDD81 pKa = 2.45DD82 pKa = 3.22WAFMWTGKK90 pKa = 10.33KK91 pKa = 10.12AFQDD95 pKa = 2.91WDD97 pKa = 3.57LDD99 pKa = 3.86NRR101 pKa = 11.84DD102 pKa = 3.43GEE104 pKa = 4.66AFGHH108 pKa = 5.46RR109 pKa = 11.84LVVNEE114 pKa = 4.55DD115 pKa = 3.04MGVQLNNLDD124 pKa = 3.88EE125 pKa = 4.73GQLVPFTYY133 pKa = 10.39LWINYY138 pKa = 8.8LGAGQSNFFIDD149 pKa = 4.06DD150 pKa = 3.69ALSFDD155 pKa = 5.37LSFDD159 pKa = 3.61NQGWFIWDD167 pKa = 3.52CDD169 pKa = 3.92PNAFTII175 pKa = 4.18

Molecular weight: 19.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W3X3N2|W3X3N2_PESFW Voltage-gated potassium channel subunit beta OS=Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) OX=1229662 GN=PFICI_09599 PE=3 SV=1
MM1 pKa = 7.41SHH3 pKa = 6.25EE4 pKa = 4.71SVWNSRR10 pKa = 11.84PRR12 pKa = 11.84SYY14 pKa = 11.57GKK16 pKa = 10.12GARR19 pKa = 11.84QCRR22 pKa = 11.84VCTHH26 pKa = 6.53KK27 pKa = 11.0AGLIRR32 pKa = 11.84KK33 pKa = 8.76YY34 pKa = 10.73GLNICRR40 pKa = 11.84QCFRR44 pKa = 11.84EE45 pKa = 4.01KK46 pKa = 10.63SADD49 pKa = 3.07IGFVKK54 pKa = 10.35HH55 pKa = 5.95RR56 pKa = 4.18

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15412

0

15412

7589864

49

9212

492.5

54.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.872 ± 0.017

1.267 ± 0.008

5.902 ± 0.013

5.931 ± 0.016

3.783 ± 0.011

6.991 ± 0.018

2.399 ± 0.01

5.051 ± 0.013

4.593 ± 0.017

8.914 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.007

3.74 ± 0.01

5.646 ± 0.017

4.044 ± 0.013

5.729 ± 0.015

8.17 ± 0.021

6.098 ± 0.018

6.186 ± 0.013

1.601 ± 0.008

2.875 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski