Coriobacteriaceae bacterium BV3Ac1
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1610 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U2VJA7|U2VJA7_9ACTN Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD OS=Coriobacteriaceae bacterium BV3Ac1 OX=1111135 GN=gatD PE=3 SV=1
MM1 pKa = 7.39 QDD3 pKa = 3.43 LVRR6 pKa = 11.84 LNVPAEE12 pKa = 3.67 PHH14 pKa = 5.24 YY15 pKa = 10.84 ARR17 pKa = 11.84 SVRR20 pKa = 11.84 MLAATLAVSCGMSVDD35 pKa = 4.6 DD36 pKa = 5.29 IEE38 pKa = 4.55 DD39 pKa = 3.06 VRR41 pKa = 11.84 MAAEE45 pKa = 3.91 EE46 pKa = 4.54 GFVYY50 pKa = 10.53 ACSTAPKK57 pKa = 8.77 TCGINFGLAPNEE69 pKa = 3.8 VTIDD73 pKa = 3.68 FDD75 pKa = 5.65 LGLQEE80 pKa = 5.72 APLDD84 pKa = 3.89 QEE86 pKa = 4.32 GDD88 pKa = 3.55 AEE90 pKa = 4.58 LVGDD94 pKa = 4.86 LYY96 pKa = 11.48 LVALLLEE103 pKa = 4.5 AVCDD107 pKa = 3.92 EE108 pKa = 4.63 FSLIDD113 pKa = 3.88 SDD115 pKa = 3.74 EE116 pKa = 4.04 GSYY119 pKa = 10.85 LHH121 pKa = 6.56 LVKK124 pKa = 10.19 RR125 pKa = 11.84 TAHH128 pKa = 5.74 VEE130 pKa = 3.86
Molecular weight: 14.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.813
IPC2_protein 4.037
IPC_protein 3.986
Toseland 3.795
ProMoST 4.088
Dawson 3.961
Bjellqvist 4.151
Wikipedia 3.859
Rodwell 3.821
Grimsley 3.706
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.253
Thurlkill 3.834
EMBOSS 3.872
Sillero 4.101
Patrickios 1.926
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 4.007
Protein with the highest isoelectric point:
>tr|U2U3H4|U2U3H4_9ACTN IstB-like ATP-binding protein OS=Coriobacteriaceae bacterium BV3Ac1 OX=1111135 GN=HMPREF1248_0999 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.27 GGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.55 GRR39 pKa = 11.84 KK40 pKa = 9.03 KK41 pKa = 10.09 LTVV44 pKa = 3.1
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1610
0
1610
533666
37
2021
331.5
36.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.428 ± 0.079
1.475 ± 0.027
6.058 ± 0.048
6.302 ± 0.058
3.721 ± 0.041
7.483 ± 0.054
2.22 ± 0.028
5.741 ± 0.061
4.581 ± 0.055
9.723 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.606 ± 0.031
3.274 ± 0.04
3.981 ± 0.036
3.468 ± 0.035
5.521 ± 0.063
6.374 ± 0.052
5.52 ± 0.052
7.459 ± 0.059
1.088 ± 0.02
2.976 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here