Lachnospiraceae bacterium G11

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5VTU2|A0A1G5VTU2_9FIRM Predicted dehydrogenase OS=Lachnospiraceae bacterium G11 OX=1200747 GN=SAMN02910368_00830 PE=4 SV=1
MM1 pKa = 6.76VRR3 pKa = 11.84KK4 pKa = 9.61FIKK7 pKa = 9.26KK8 pKa = 6.74TALIIMATMMLTACGAEE25 pKa = 4.07EE26 pKa = 4.15EE27 pKa = 4.57NAGFDD32 pKa = 3.34PGEE35 pKa = 4.37YY36 pKa = 9.44EE37 pKa = 4.83SCWTDD42 pKa = 3.0EE43 pKa = 4.78DD44 pKa = 4.32GDD46 pKa = 4.49LIKK49 pKa = 10.88LIPSEE54 pKa = 3.6NSYY57 pKa = 10.78IFRR60 pKa = 11.84KK61 pKa = 10.02ADD63 pKa = 3.16GRR65 pKa = 11.84VGRR68 pKa = 11.84GTYY71 pKa = 8.06TVNNSQLTYY80 pKa = 11.23DD81 pKa = 3.77RR82 pKa = 11.84FIYY85 pKa = 10.42DD86 pKa = 3.2IVKK89 pKa = 10.9DD90 pKa = 3.98GDD92 pKa = 3.77DD93 pKa = 4.14KK94 pKa = 11.5FYY96 pKa = 11.02LAQNGSTTNTTTEE109 pKa = 3.84ILDD112 pKa = 3.97GYY114 pKa = 11.0VFTKK118 pKa = 9.93IDD120 pKa = 3.28AVEE123 pKa = 3.83IDD125 pKa = 4.72EE126 pKa = 4.73YY127 pKa = 11.02PNDD130 pKa = 3.99LLDD133 pKa = 4.43GVWVSDD139 pKa = 2.84TGTRR143 pKa = 11.84LEE145 pKa = 3.76IDD147 pKa = 3.36MDD149 pKa = 3.2ISEE152 pKa = 4.55YY153 pKa = 10.82SVSHH157 pKa = 6.21EE158 pKa = 4.41NGSGMGTVFDD168 pKa = 4.26YY169 pKa = 11.66GDD171 pKa = 3.48GKK173 pKa = 10.98GVYY176 pKa = 10.29LFATDD181 pKa = 3.83FAYY184 pKa = 10.74VIWIDD189 pKa = 3.22SDD191 pKa = 4.09NITFEE196 pKa = 4.26STDD199 pKa = 3.5PVYY202 pKa = 11.36ADD204 pKa = 3.09ATFTRR209 pKa = 11.84EE210 pKa = 3.38TDD212 pKa = 3.47SYY214 pKa = 11.43GEE216 pKa = 4.43EE217 pKa = 4.31YY218 pKa = 10.92DD219 pKa = 4.99DD220 pKa = 4.61GG221 pKa = 5.68

Molecular weight: 24.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5XY58|A0A1G5XY58_9FIRM UPF0365 protein SAMN02910368_02441 OS=Lachnospiraceae bacterium G11 OX=1200747 GN=SAMN02910368_02441 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.63NRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.89VHH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.77VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84KK40 pKa = 8.69ILSAA44 pKa = 4.02

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2732

0

2732

939179

40

3717

343.8

38.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.5 ± 0.046

1.361 ± 0.016

6.256 ± 0.037

7.606 ± 0.048

4.407 ± 0.035

7.17 ± 0.04

1.489 ± 0.017

7.679 ± 0.046

7.164 ± 0.04

8.693 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.992 ± 0.02

4.676 ± 0.032

3.226 ± 0.022

2.274 ± 0.021

3.953 ± 0.032

5.874 ± 0.036

5.427 ± 0.03

7.069 ± 0.035

0.89 ± 0.015

4.295 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski