Staphylococcus phage IME1348_01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rockefellervirus; Staphylococcus virus IME1348-01

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JP90|A0A1W6JP90_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1348_01 OX=1965489 PE=4 SV=1
MM1 pKa = 7.41IVYY4 pKa = 10.1LPHH7 pKa = 6.78YY8 pKa = 10.43DD9 pKa = 4.23NGEE12 pKa = 4.08EE13 pKa = 4.21TCNNSHH19 pKa = 6.13GVSDD23 pKa = 3.6KK24 pKa = 11.22AYY26 pKa = 9.41TSLEE30 pKa = 4.03DD31 pKa = 4.43AIQEE35 pKa = 4.14IKK37 pKa = 10.8EE38 pKa = 3.9MGYY41 pKa = 10.38VIQEE45 pKa = 4.27DD46 pKa = 3.59KK47 pKa = 11.14SDD49 pKa = 3.73EE50 pKa = 3.99IEE52 pKa = 4.3YY53 pKa = 9.86MGFPDD58 pKa = 4.64IEE60 pKa = 4.46INSEE64 pKa = 3.54ILPYY68 pKa = 10.85AFYY71 pKa = 10.7KK72 pKa = 10.91VPFEE76 pKa = 4.38SYY78 pKa = 10.29RR79 pKa = 11.84KK80 pKa = 7.39TSTYY84 pKa = 10.33FFDD87 pKa = 4.08DD88 pKa = 3.96RR89 pKa = 11.84EE90 pKa = 3.91FAFINKK96 pKa = 9.06IEE98 pKa = 4.09LVEE101 pKa = 4.71GEE103 pKa = 4.33TDD105 pKa = 3.61DD106 pKa = 4.58

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JP92|A0A1W6JP92_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1348_01 OX=1965489 PE=4 SV=1
MM1 pKa = 7.54SLTPNEE7 pKa = 3.89WKK9 pKa = 10.31DD10 pKa = 3.28WVVGRR15 pKa = 11.84RR16 pKa = 11.84LALLDD21 pKa = 3.78EE22 pKa = 4.42QEE24 pKa = 4.32TLLFGAQANGLVQAGKK40 pKa = 9.58SLKK43 pKa = 10.18RR44 pKa = 11.84LQKK47 pKa = 8.59QLEE50 pKa = 3.97RR51 pKa = 11.84ARR53 pKa = 11.84YY54 pKa = 6.86EE55 pKa = 3.64VRR57 pKa = 11.84GQSEE61 pKa = 4.38EE62 pKa = 4.06YY63 pKa = 10.73EE64 pKa = 4.03RR65 pKa = 11.84MKK67 pKa = 10.59EE68 pKa = 4.04RR69 pKa = 11.84KK70 pKa = 8.73LAHH73 pKa = 6.28NKK75 pKa = 10.13RR76 pKa = 11.84IRR78 pKa = 11.84NVQKK82 pKa = 9.93QGTRR86 pKa = 11.84RR87 pKa = 11.84FMNSLRR93 pKa = 11.84NTSQKK98 pKa = 11.01GGG100 pKa = 3.49

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13395

46

1034

212.6

24.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.487 ± 0.495

0.635 ± 0.116

6.383 ± 0.386

7.227 ± 0.524

3.8 ± 0.199

6.092 ± 0.357

1.732 ± 0.172

7.204 ± 0.26

9.265 ± 0.426

7.368 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.174

7.159 ± 0.335

2.785 ± 0.17

4.293 ± 0.296

4.487 ± 0.259

5.793 ± 0.282

5.95 ± 0.274

6.002 ± 0.229

1.433 ± 0.158

4.584 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski