Amino acid dipepetide frequency for Staphylococcus phage IME1348_01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.24AlaAla: 2.24 ± 0.449
0.299AlaCys: 0.299 ± 0.141
2.688AlaAsp: 2.688 ± 0.552
3.36AlaGlu: 3.36 ± 0.508
2.613AlaPhe: 2.613 ± 0.717
3.285AlaGly: 3.285 ± 0.639
1.269AlaHis: 1.269 ± 0.317
5.226AlaIle: 5.226 ± 0.787
5.6AlaLys: 5.6 ± 0.742
4.629AlaLeu: 4.629 ± 0.882
1.717AlaMet: 1.717 ± 0.37
4.032AlaAsn: 4.032 ± 0.537
1.419AlaPro: 1.419 ± 0.294
2.314AlaGln: 2.314 ± 0.521
1.717AlaArg: 1.717 ± 0.276
4.032AlaSer: 4.032 ± 0.901
2.986AlaThr: 2.986 ± 0.532
2.464AlaVal: 2.464 ± 0.589
0.821AlaTrp: 0.821 ± 0.228
2.165AlaTyr: 2.165 ± 0.347
0.0AlaXaa: 0.0 ± 0.0
Cys
0.224CysAla: 0.224 ± 0.123
0.0CysCys: 0.0 ± 0.0
0.149CysAsp: 0.149 ± 0.117
0.224CysGlu: 0.224 ± 0.117
0.149CysPhe: 0.149 ± 0.098
0.523CysGly: 0.523 ± 0.17
0.075CysHis: 0.075 ± 0.077
0.373CysIle: 0.373 ± 0.219
0.597CysLys: 0.597 ± 0.28
0.597CysLeu: 0.597 ± 0.194
0.075CysMet: 0.075 ± 0.071
0.821CysAsn: 0.821 ± 0.24
0.299CysPro: 0.299 ± 0.16
0.149CysGln: 0.149 ± 0.099
0.299CysArg: 0.299 ± 0.146
0.821CysSer: 0.821 ± 0.344
0.075CysThr: 0.075 ± 0.077
0.448CysVal: 0.448 ± 0.157
0.075CysTrp: 0.075 ± 0.063
0.373CysTyr: 0.373 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
4.106AspAla: 4.106 ± 0.751
0.448AspCys: 0.448 ± 0.192
4.554AspAsp: 4.554 ± 0.673
6.346AspGlu: 6.346 ± 0.749
2.389AspPhe: 2.389 ± 0.348
3.957AspGly: 3.957 ± 0.487
0.672AspHis: 0.672 ± 0.24
4.778AspIle: 4.778 ± 0.707
5.45AspLys: 5.45 ± 0.554
5.749AspLeu: 5.749 ± 0.778
1.269AspMet: 1.269 ± 0.322
4.928AspAsn: 4.928 ± 0.673
1.045AspPro: 1.045 ± 0.272
0.971AspGln: 0.971 ± 0.226
2.016AspArg: 2.016 ± 0.396
2.688AspSer: 2.688 ± 0.408
4.33AspThr: 4.33 ± 0.647
4.032AspVal: 4.032 ± 0.616
0.821AspTrp: 0.821 ± 0.215
3.285AspTyr: 3.285 ± 0.574
0.0AspXaa: 0.0 ± 0.0
Glu
2.613GluAla: 2.613 ± 0.377
0.523GluCys: 0.523 ± 0.201
3.658GluAsp: 3.658 ± 0.587
5.301GluGlu: 5.301 ± 0.78
2.986GluPhe: 2.986 ± 0.471
3.136GluGly: 3.136 ± 0.64
1.493GluHis: 1.493 ± 0.377
5.525GluIle: 5.525 ± 0.696
5.45GluLys: 5.45 ± 0.668
7.167GluLeu: 7.167 ± 0.948
1.643GluMet: 1.643 ± 0.378
4.554GluAsn: 4.554 ± 0.559
1.419GluPro: 1.419 ± 0.403
3.882GluGln: 3.882 ± 0.686
4.554GluArg: 4.554 ± 0.742
4.106GluSer: 4.106 ± 0.55
3.882GluThr: 3.882 ± 0.519
5.226GluVal: 5.226 ± 0.535
1.568GluTrp: 1.568 ± 0.356
3.584GluTyr: 3.584 ± 0.418
0.0GluXaa: 0.0 ± 0.0
Phe
1.941PheAla: 1.941 ± 0.388
0.149PheCys: 0.149 ± 0.106
2.538PheAsp: 2.538 ± 0.396
2.613PheGlu: 2.613 ± 0.456
1.344PhePhe: 1.344 ± 0.293
2.464PheGly: 2.464 ± 0.753
0.821PheHis: 0.821 ± 0.211
3.061PheIle: 3.061 ± 0.546
3.285PheLys: 3.285 ± 0.437
1.941PheLeu: 1.941 ± 0.441
1.419PheMet: 1.419 ± 0.35
3.061PheAsn: 3.061 ± 0.399
0.672PhePro: 0.672 ± 0.22
1.344PheGln: 1.344 ± 0.338
1.643PheArg: 1.643 ± 0.394
2.165PheSer: 2.165 ± 0.472
2.538PheThr: 2.538 ± 0.429
2.314PheVal: 2.314 ± 0.417
0.373PheTrp: 0.373 ± 0.203
2.314PheTyr: 2.314 ± 0.58
0.0PheXaa: 0.0 ± 0.0
Gly
4.032GlyAla: 4.032 ± 0.75
0.672GlyCys: 0.672 ± 0.222
2.912GlyAsp: 2.912 ± 0.547
3.285GlyGlu: 3.285 ± 0.53
2.389GlyPhe: 2.389 ± 0.388
3.36GlyGly: 3.36 ± 0.685
1.344GlyHis: 1.344 ± 0.412
4.704GlyIle: 4.704 ± 0.872
5.152GlyLys: 5.152 ± 0.648
4.405GlyLeu: 4.405 ± 0.692
1.643GlyMet: 1.643 ± 0.391
4.405GlyAsn: 4.405 ± 0.651
0.597GlyPro: 0.597 ± 0.188
2.986GlyGln: 2.986 ± 0.451
2.986GlyArg: 2.986 ± 0.348
3.061GlySer: 3.061 ± 0.695
3.808GlyThr: 3.808 ± 0.537
4.853GlyVal: 4.853 ± 0.545
0.821GlyTrp: 0.821 ± 0.276
3.509GlyTyr: 3.509 ± 0.585
0.0GlyXaa: 0.0 ± 0.0
His
0.896HisAla: 0.896 ± 0.3
0.075HisCys: 0.075 ± 0.069
0.821HisAsp: 0.821 ± 0.238
0.821HisGlu: 0.821 ± 0.206
0.672HisPhe: 0.672 ± 0.208
1.269HisGly: 1.269 ± 0.275
0.821HisHis: 0.821 ± 0.379
1.045HisIle: 1.045 ± 0.309
1.195HisLys: 1.195 ± 0.265
1.717HisLeu: 1.717 ± 0.367
0.224HisMet: 0.224 ± 0.105
1.195HisAsn: 1.195 ± 0.256
0.747HisPro: 0.747 ± 0.226
0.373HisGln: 0.373 ± 0.163
0.448HisArg: 0.448 ± 0.219
1.269HisSer: 1.269 ± 0.255
1.568HisThr: 1.568 ± 0.33
1.12HisVal: 1.12 ± 0.244
0.075HisTrp: 0.075 ± 0.079
0.971HisTyr: 0.971 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
5.002IleAla: 5.002 ± 0.814
0.224IleCys: 0.224 ± 0.131
5.6IleAsp: 5.6 ± 0.825
5.749IleGlu: 5.749 ± 0.738
2.314IlePhe: 2.314 ± 0.475
5.376IleGly: 5.376 ± 0.741
0.597IleHis: 0.597 ± 0.223
3.882IleIle: 3.882 ± 0.728
9.706IleLys: 9.706 ± 0.896
4.106IleLeu: 4.106 ± 0.643
1.344IleMet: 1.344 ± 0.414
4.629IleAsn: 4.629 ± 0.569
2.613IlePro: 2.613 ± 0.361
2.314IleGln: 2.314 ± 0.44
2.24IleArg: 2.24 ± 0.306
4.629IleSer: 4.629 ± 0.888
4.853IleThr: 4.853 ± 0.692
2.986IleVal: 2.986 ± 0.547
1.195IleTrp: 1.195 ± 0.346
2.688IleTyr: 2.688 ± 0.495
0.0IleXaa: 0.0 ± 0.0
Lys
5.376LysAla: 5.376 ± 0.707
0.448LysCys: 0.448 ± 0.239
6.197LysAsp: 6.197 ± 0.694
7.989LysGlu: 7.989 ± 1.091
3.658LysPhe: 3.658 ± 0.441
5.749LysGly: 5.749 ± 0.544
1.941LysHis: 1.941 ± 0.325
5.002LysIle: 5.002 ± 0.686
6.719LysLys: 6.719 ± 0.982
7.242LysLeu: 7.242 ± 0.757
2.613LysMet: 2.613 ± 0.456
6.495LysAsn: 6.495 ± 0.87
2.688LysPro: 2.688 ± 0.631
4.629LysGln: 4.629 ± 0.556
4.48LysArg: 4.48 ± 0.632
4.853LysSer: 4.853 ± 0.701
6.346LysThr: 6.346 ± 0.817
4.48LysVal: 4.48 ± 0.576
0.971LysTrp: 0.971 ± 0.228
4.778LysTyr: 4.778 ± 0.813
0.0LysXaa: 0.0 ± 0.0
Leu
4.032LeuAla: 4.032 ± 0.662
0.448LeuCys: 0.448 ± 0.215
5.749LeuAsp: 5.749 ± 0.691
5.749LeuGlu: 5.749 ± 0.818
2.688LeuPhe: 2.688 ± 0.448
4.405LeuGly: 4.405 ± 0.741
1.568LeuHis: 1.568 ± 0.367
4.853LeuIle: 4.853 ± 0.729
7.466LeuLys: 7.466 ± 0.788
4.33LeuLeu: 4.33 ± 0.557
1.643LeuMet: 1.643 ± 0.339
5.301LeuAsn: 5.301 ± 0.546
2.389LeuPro: 2.389 ± 0.424
4.106LeuGln: 4.106 ± 0.6
3.882LeuArg: 3.882 ± 0.685
4.405LeuSer: 4.405 ± 0.565
3.21LeuThr: 3.21 ± 0.425
3.509LeuVal: 3.509 ± 0.436
1.045LeuTrp: 1.045 ± 0.338
2.912LeuTyr: 2.912 ± 0.576
0.0LeuXaa: 0.0 ± 0.0
Met
1.568MetAla: 1.568 ± 0.457
0.075MetCys: 0.075 ± 0.07
1.12MetAsp: 1.12 ± 0.282
1.493MetGlu: 1.493 ± 0.345
0.896MetPhe: 0.896 ± 0.294
1.195MetGly: 1.195 ± 0.322
0.149MetHis: 0.149 ± 0.108
1.867MetIle: 1.867 ± 0.308
2.389MetLys: 2.389 ± 0.334
1.792MetLeu: 1.792 ± 0.447
0.448MetMet: 0.448 ± 0.206
1.867MetAsn: 1.867 ± 0.348
0.821MetPro: 0.821 ± 0.236
0.747MetGln: 0.747 ± 0.224
0.971MetArg: 0.971 ± 0.303
1.493MetSer: 1.493 ± 0.32
1.493MetThr: 1.493 ± 0.314
0.971MetVal: 0.971 ± 0.222
0.597MetTrp: 0.597 ± 0.21
1.269MetTyr: 1.269 ± 0.383
0.0MetXaa: 0.0 ± 0.0
Asn
3.509AsnAla: 3.509 ± 0.583
0.896AsnCys: 0.896 ± 0.385
4.48AsnAsp: 4.48 ± 0.559
4.554AsnGlu: 4.554 ± 0.552
2.762AsnPhe: 2.762 ± 0.466
5.376AsnGly: 5.376 ± 0.8
1.195AsnHis: 1.195 ± 0.273
4.778AsnIle: 4.778 ± 0.834
6.271AsnLys: 6.271 ± 0.913
4.554AsnLeu: 4.554 ± 0.679
0.896AsnMet: 0.896 ± 0.286
6.57AsnAsn: 6.57 ± 1.039
2.24AsnPro: 2.24 ± 0.408
3.584AsnGln: 3.584 ± 0.746
3.36AsnArg: 3.36 ± 0.603
4.106AsnSer: 4.106 ± 0.444
4.032AsnThr: 4.032 ± 0.71
4.629AsnVal: 4.629 ± 0.644
0.896AsnTrp: 0.896 ± 0.256
2.912AsnTyr: 2.912 ± 0.592
0.0AsnXaa: 0.0 ± 0.0
Pro
1.195ProAla: 1.195 ± 0.251
0.075ProCys: 0.075 ± 0.075
1.941ProAsp: 1.941 ± 0.428
1.419ProGlu: 1.419 ± 0.294
1.643ProPhe: 1.643 ± 0.337
1.045ProGly: 1.045 ± 0.359
0.448ProHis: 0.448 ± 0.179
2.762ProIle: 2.762 ± 0.626
2.912ProLys: 2.912 ± 0.51
1.045ProLeu: 1.045 ± 0.254
0.523ProMet: 0.523 ± 0.196
1.643ProAsn: 1.643 ± 0.417
1.195ProPro: 1.195 ± 0.329
1.195ProGln: 1.195 ± 0.371
1.792ProArg: 1.792 ± 0.325
2.688ProSer: 2.688 ± 0.579
1.269ProThr: 1.269 ± 0.392
1.643ProVal: 1.643 ± 0.38
0.224ProTrp: 0.224 ± 0.127
1.195ProTyr: 1.195 ± 0.288
0.0ProXaa: 0.0 ± 0.0
Gln
2.986GlnAla: 2.986 ± 0.559
0.299GlnCys: 0.299 ± 0.128
2.314GlnAsp: 2.314 ± 0.402
2.837GlnGlu: 2.837 ± 0.57
1.344GlnPhe: 1.344 ± 0.279
2.24GlnGly: 2.24 ± 0.43
0.672GlnHis: 0.672 ± 0.195
3.061GlnIle: 3.061 ± 0.476
4.554GlnLys: 4.554 ± 0.733
2.688GlnLeu: 2.688 ± 0.43
1.195GlnMet: 1.195 ± 0.246
3.434GlnAsn: 3.434 ± 0.587
1.568GlnPro: 1.568 ± 0.422
2.986GlnGln: 2.986 ± 0.936
2.165GlnArg: 2.165 ± 0.383
2.389GlnSer: 2.389 ± 0.418
2.464GlnThr: 2.464 ± 0.446
1.867GlnVal: 1.867 ± 0.466
0.523GlnTrp: 0.523 ± 0.187
1.344GlnTyr: 1.344 ± 0.329
0.0GlnXaa: 0.0 ± 0.0
Arg
1.792ArgAla: 1.792 ± 0.493
0.224ArgCys: 0.224 ± 0.143
3.285ArgAsp: 3.285 ± 0.55
3.21ArgGlu: 3.21 ± 0.581
1.12ArgPhe: 1.12 ± 0.275
2.165ArgGly: 2.165 ± 0.423
0.672ArgHis: 0.672 ± 0.193
3.136ArgIle: 3.136 ± 0.433
3.882ArgLys: 3.882 ± 0.6
3.584ArgLeu: 3.584 ± 0.478
1.493ArgMet: 1.493 ± 0.303
2.912ArgAsn: 2.912 ± 0.378
0.971ArgPro: 0.971 ± 0.232
1.867ArgGln: 1.867 ± 0.413
2.464ArgArg: 2.464 ± 0.5
2.762ArgSer: 2.762 ± 0.413
1.941ArgThr: 1.941 ± 0.529
3.658ArgVal: 3.658 ± 0.473
0.971ArgTrp: 0.971 ± 0.313
2.688ArgTyr: 2.688 ± 0.489
0.0ArgXaa: 0.0 ± 0.0
Ser
3.285SerAla: 3.285 ± 0.696
0.224SerCys: 0.224 ± 0.136
3.658SerAsp: 3.658 ± 0.552
3.509SerGlu: 3.509 ± 0.454
1.792SerPhe: 1.792 ± 0.376
4.48SerGly: 4.48 ± 0.787
0.971SerHis: 0.971 ± 0.259
4.181SerIle: 4.181 ± 0.6
4.629SerLys: 4.629 ± 0.549
4.928SerLeu: 4.928 ± 0.623
1.419SerMet: 1.419 ± 0.309
4.48SerAsn: 4.48 ± 0.751
1.269SerPro: 1.269 ± 0.286
2.314SerGln: 2.314 ± 0.461
2.986SerArg: 2.986 ± 0.561
2.314SerSer: 2.314 ± 0.454
3.285SerThr: 3.285 ± 0.555
3.808SerVal: 3.808 ± 0.463
1.045SerTrp: 1.045 ± 0.299
3.36SerTyr: 3.36 ± 0.526
0.0SerXaa: 0.0 ± 0.0
Thr
3.658ThrAla: 3.658 ± 0.551
0.373ThrCys: 0.373 ± 0.179
3.285ThrAsp: 3.285 ± 0.399
3.434ThrGlu: 3.434 ± 0.659
3.21ThrPhe: 3.21 ± 0.511
3.584ThrGly: 3.584 ± 0.583
0.896ThrHis: 0.896 ± 0.246
5.152ThrIle: 5.152 ± 0.595
6.271ThrLys: 6.271 ± 0.946
4.778ThrLeu: 4.778 ± 0.553
0.821ThrMet: 0.821 ± 0.233
4.106ThrAsn: 4.106 ± 0.599
2.464ThrPro: 2.464 ± 0.507
2.24ThrGln: 2.24 ± 0.332
1.493ThrArg: 1.493 ± 0.494
3.061ThrSer: 3.061 ± 0.505
4.33ThrThr: 4.33 ± 0.676
3.733ThrVal: 3.733 ± 0.747
0.523ThrTrp: 0.523 ± 0.244
2.09ThrTyr: 2.09 ± 0.433
0.0ThrXaa: 0.0 ± 0.0
Val
3.733ValAla: 3.733 ± 0.695
0.299ValCys: 0.299 ± 0.189
5.002ValAsp: 5.002 ± 0.503
5.002ValGlu: 5.002 ± 0.641
1.941ValPhe: 1.941 ± 0.511
3.509ValGly: 3.509 ± 0.443
0.373ValHis: 0.373 ± 0.195
3.882ValIle: 3.882 ± 0.525
5.525ValLys: 5.525 ± 0.69
4.181ValLeu: 4.181 ± 0.796
1.269ValMet: 1.269 ± 0.294
3.434ValAsn: 3.434 ± 0.408
2.016ValPro: 2.016 ± 0.437
2.09ValGln: 2.09 ± 0.394
2.613ValArg: 2.613 ± 0.399
4.181ValSer: 4.181 ± 0.63
3.509ValThr: 3.509 ± 0.406
4.853ValVal: 4.853 ± 0.596
0.448ValTrp: 0.448 ± 0.187
2.165ValTyr: 2.165 ± 0.453
0.0ValXaa: 0.0 ± 0.0
Trp
0.597TrpAla: 0.597 ± 0.221
0.224TrpCys: 0.224 ± 0.133
0.523TrpAsp: 0.523 ± 0.209
1.568TrpGlu: 1.568 ± 0.393
0.448TrpPhe: 0.448 ± 0.183
0.896TrpGly: 0.896 ± 0.245
0.299TrpHis: 0.299 ± 0.145
1.12TrpIle: 1.12 ± 0.33
1.269TrpLys: 1.269 ± 0.329
1.269TrpLeu: 1.269 ± 0.294
0.224TrpMet: 0.224 ± 0.144
0.747TrpAsn: 0.747 ± 0.296
0.149TrpPro: 0.149 ± 0.101
0.373TrpGln: 0.373 ± 0.141
0.597TrpArg: 0.597 ± 0.183
0.747TrpSer: 0.747 ± 0.215
1.419TrpThr: 1.419 ± 0.272
0.896TrpVal: 0.896 ± 0.273
0.299TrpTrp: 0.299 ± 0.173
0.672TrpTyr: 0.672 ± 0.239
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.09TyrAla: 2.09 ± 0.407
0.373TyrCys: 0.373 ± 0.182
3.36TyrAsp: 3.36 ± 0.488
3.808TyrGlu: 3.808 ± 0.676
1.643TyrPhe: 1.643 ± 0.372
2.912TyrGly: 2.912 ± 0.651
1.045TyrHis: 1.045 ± 0.256
3.658TyrIle: 3.658 ± 0.453
4.33TyrLys: 4.33 ± 0.668
2.986TyrLeu: 2.986 ± 0.484
1.269TyrMet: 1.269 ± 0.321
2.986TyrAsn: 2.986 ± 0.485
1.269TyrPro: 1.269 ± 0.309
2.464TyrGln: 2.464 ± 0.65
2.165TyrArg: 2.165 ± 0.42
2.165TyrSer: 2.165 ± 0.321
2.165TyrThr: 2.165 ± 0.359
2.538TyrVal: 2.538 ± 0.395
1.045TyrTrp: 1.045 ± 0.295
1.568TyrTyr: 1.568 ± 0.425
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13395 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski