Crucibulum laeve

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Agaricales incertae sedis; Crucibulum

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C3LQA1|A0A5C3LQA1_9AGAR Uncharacterized protein OS=Crucibulum laeve OX=68775 GN=BDQ12DRAFT_688331 PE=4 SV=1
MM1 pKa = 7.72SNRR4 pKa = 11.84DD5 pKa = 3.67DD6 pKa = 3.63NSYY9 pKa = 11.53GSGNDD14 pKa = 3.33SYY16 pKa = 12.15GSGNNDD22 pKa = 2.84SYY24 pKa = 11.85GSSNRR29 pKa = 11.84SGDD32 pKa = 3.24NDD34 pKa = 3.42NNNRR38 pKa = 11.84RR39 pKa = 11.84SGDD42 pKa = 3.39DD43 pKa = 3.51DD44 pKa = 3.96NSYY47 pKa = 11.39GSNNNSSTNDD57 pKa = 3.14SYY59 pKa = 12.1GSGRR63 pKa = 11.84DD64 pKa = 3.38RR65 pKa = 11.84SDD67 pKa = 5.16DD68 pKa = 3.61NDD70 pKa = 3.49NTSSGNTYY78 pKa = 10.43GSSNNDD84 pKa = 2.84SYY86 pKa = 11.99GSSNTGSNKK95 pKa = 9.74EE96 pKa = 4.08SGSYY100 pKa = 10.51GSNDD104 pKa = 2.93NSYY107 pKa = 11.15GSSAKK112 pKa = 10.33DD113 pKa = 3.16SYY115 pKa = 11.66GSSNNDD121 pKa = 2.91TYY123 pKa = 11.65GSSNKK128 pKa = 9.24EE129 pKa = 3.62SSYY132 pKa = 11.1GSSSNEE138 pKa = 3.56DD139 pKa = 3.42SYY141 pKa = 12.21GSSKK145 pKa = 10.33KK146 pKa = 10.35DD147 pKa = 3.13SYY149 pKa = 11.79GSNNDD154 pKa = 3.3SYY156 pKa = 11.89GSSNNDD162 pKa = 3.11SNKK165 pKa = 9.33GDD167 pKa = 3.83SYY169 pKa = 11.77GSSNNNRR176 pKa = 11.84SSNEE180 pKa = 3.55DD181 pKa = 3.27SYY183 pKa = 12.16GSNNEE188 pKa = 3.64SSYY191 pKa = 11.54GNNDD195 pKa = 3.26NEE197 pKa = 4.72SSNNNSNNNNNSQGKK212 pKa = 9.61SGDD215 pKa = 3.5WVDD218 pKa = 4.15KK219 pKa = 10.29GVSYY223 pKa = 10.58AAKK226 pKa = 8.81QAGYY230 pKa = 10.46NVDD233 pKa = 3.36EE234 pKa = 4.51ATADD238 pKa = 3.7KK239 pKa = 10.37IGNSISQGLGKK250 pKa = 10.18FVGGGPGGILL260 pKa = 3.44

Molecular weight: 26.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C3M7Z2|A0A5C3M7Z2_9AGAR Transmembrane amino acid transporter protein-domain-containing protein OS=Crucibulum laeve OX=68775 GN=BDQ12DRAFT_710842 PE=3 SV=1
MM1 pKa = 7.95LMRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.6KK7 pKa = 10.26RR8 pKa = 11.84NLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.5LPQGRR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 9.61QKK22 pKa = 10.23LRR24 pKa = 11.84TRR26 pKa = 11.84RR27 pKa = 11.84AARR30 pKa = 11.84RR31 pKa = 11.84VRR33 pKa = 11.84SPRR36 pKa = 11.84QSVPLPKK43 pKa = 9.9KK44 pKa = 9.8RR45 pKa = 11.84LSPRR49 pKa = 11.84KK50 pKa = 8.85RR51 pKa = 11.84LLPRR55 pKa = 11.84SVLPRR60 pKa = 11.84KK61 pKa = 9.44RR62 pKa = 11.84RR63 pKa = 11.84PNQRR67 pKa = 11.84TSLEE71 pKa = 4.23KK72 pKa = 10.26ISPKK76 pKa = 10.23RR77 pKa = 11.84STPCPPIPTTKK88 pKa = 10.55LSLRR92 pKa = 11.84KK93 pKa = 9.43RR94 pKa = 11.84NPRR97 pKa = 11.84KK98 pKa = 9.74RR99 pKa = 11.84PLLRR103 pKa = 11.84RR104 pKa = 11.84SPPRR108 pKa = 11.84KK109 pKa = 9.38LLHH112 pKa = 5.7LRR114 pKa = 11.84LRR116 pKa = 11.84KK117 pKa = 9.29RR118 pKa = 11.84RR119 pKa = 11.84LSLRR123 pKa = 11.84RR124 pKa = 11.84KK125 pKa = 7.9QPQRR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84MRR133 pKa = 11.84KK134 pKa = 9.18RR135 pKa = 11.84MSIWRR140 pKa = 11.84MPLSTKK146 pKa = 9.25RR147 pKa = 11.84RR148 pKa = 11.84RR149 pKa = 11.84VVASARR155 pKa = 11.84SLHH158 pKa = 6.75RR159 pKa = 11.84KK160 pKa = 8.59RR161 pKa = 11.84QLSLPRR167 pKa = 11.84RR168 pKa = 11.84KK169 pKa = 9.64PNPPPRR175 pKa = 11.84QRR177 pKa = 11.84RR178 pKa = 11.84ARR180 pKa = 11.84KK181 pKa = 8.7LLRR184 pKa = 11.84MMEE187 pKa = 4.3TMLMTDD193 pKa = 3.96DD194 pKa = 4.3YY195 pKa = 11.31LPVVPLQCFISFDD208 pKa = 3.42

Molecular weight: 25.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14180

0

14180

5698666

49

5063

401.9

44.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.089 ± 0.019

1.256 ± 0.008

5.31 ± 0.014

5.759 ± 0.021

3.867 ± 0.014

6.378 ± 0.02

2.547 ± 0.009

5.424 ± 0.015

4.719 ± 0.021

9.334 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.008

3.725 ± 0.012

6.335 ± 0.03

3.706 ± 0.015

5.733 ± 0.018

9.033 ± 0.026

6.212 ± 0.015

6.21 ± 0.015

1.433 ± 0.008

2.786 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski