Altererythrobacter salegens
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3438 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I4SWT7|A0A6I4SWT7_9SPHN Ribonuclease PH OS=Altererythrobacter salegens OX=1737568 GN=rph PE=3 SV=1
MM1 pKa = 7.55 SKK3 pKa = 9.98 ILRR6 pKa = 11.84 YY7 pKa = 9.29 AAAGVAMASLGVASAANAATTDD29 pKa = 3.82 SADD32 pKa = 3.31 VTATILTALSVDD44 pKa = 3.52 VDD46 pKa = 4.11 PAADD50 pKa = 3.55 TLNFGTIADD59 pKa = 4.07 GGITANQTITVSTAGVRR76 pKa = 11.84 GACPTGLICGGTTAAPKK93 pKa = 10.6 FNVTGLGGLAVNVSFVNTSEE113 pKa = 4.04 TLSYY117 pKa = 10.22 DD118 pKa = 3.35 TVANGGAAPAGFNSTMTVNNFQTDD142 pKa = 3.69 ALNGAVTNQLLLSAGGTGSFNVGGSLIVQPNMAPGVYY179 pKa = 9.91 SGTLTVSVAYY189 pKa = 10.34 NN190 pKa = 3.2
Molecular weight: 18.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.935
IPC_protein 3.846
Toseland 3.617
ProMoST 4.075
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.897
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.808
Nozaki 4.024
DTASelect 4.317
Thurlkill 3.719
EMBOSS 3.884
Sillero 3.986
Patrickios 0.693
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|A0A6I4SXS6|A0A6I4SXS6_9SPHN MarR family transcriptional regulator OS=Altererythrobacter salegens OX=1737568 GN=GRI89_10355 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.51 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 7.81 ATVGGRR28 pKa = 11.84 KK29 pKa = 8.12 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.81 KK41 pKa = 10.46 LCAA44 pKa = 3.96
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.442
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3438
0
3438
1082438
40
3228
314.8
34.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.548 ± 0.062
0.842 ± 0.013
6.031 ± 0.032
6.129 ± 0.038
3.735 ± 0.029
9.041 ± 0.047
2.005 ± 0.021
4.814 ± 0.025
3.256 ± 0.032
9.741 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.462 ± 0.022
2.647 ± 0.027
5.328 ± 0.035
3.03 ± 0.021
6.778 ± 0.04
5.345 ± 0.03
5.171 ± 0.029
7.17 ± 0.034
1.568 ± 0.017
2.361 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here