Aeromonas virus 25
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 242 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q19CT6|Q19CT6_9CAUD Uncharacterized protein OS=Aeromonas virus 25 OX=233894 GN=PHG25ORF080c PE=4 SV=1
MM1 pKa = 7.12 KK2 pKa = 10.08 HH3 pKa = 5.44 VNEE6 pKa = 4.4 MPKK9 pKa = 9.92 NGQFVVVWVYY19 pKa = 11.06 DD20 pKa = 3.54 GKK22 pKa = 10.54 IWSEE26 pKa = 3.49 TWRR29 pKa = 11.84 WNDD32 pKa = 3.07 GVVEE36 pKa = 4.12 ILLDD40 pKa = 3.55 DD41 pKa = 4.0 TAAEE45 pKa = 4.09 EE46 pKa = 4.34 WRR48 pKa = 11.84 TNDD51 pKa = 3.33 CYY53 pKa = 11.55 YY54 pKa = 11.29 DD55 pKa = 4.33 DD56 pKa = 5.33 GYY58 pKa = 11.71 EE59 pKa = 4.08 DD60 pKa = 5.57 LEE62 pKa = 4.54 LNYY65 pKa = 10.03 IVII68 pKa = 4.43
Molecular weight: 8.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 4.024
IPC_protein 3.935
Toseland 3.745
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.859
Nozaki 4.05
DTASelect 4.228
Thurlkill 3.795
EMBOSS 3.859
Sillero 4.062
Patrickios 1.914
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.949
Protein with the highest isoelectric point:
>tr|Q19CR8|Q19CR8_9CAUD Site-specific RNase OS=Aeromonas virus 25 OX=233894 GN=regB PE=4 SV=1
MM1 pKa = 7.06 MPEE4 pKa = 3.85 NLMLLRR10 pKa = 11.84 APGGRR15 pKa = 11.84 TRR17 pKa = 11.84 RR18 pKa = 11.84 PRR20 pKa = 11.84 EE21 pKa = 3.62 LVKK24 pKa = 10.6 FLSLWNNIGPRR35 pKa = 11.84 KK36 pKa = 8.25 TIGVTKK42 pKa = 9.71 EE43 pKa = 3.86 QVPEE47 pKa = 3.94 EE48 pKa = 4.04 QLTEE52 pKa = 3.88 EE53 pKa = 3.92 QKK55 pKa = 10.25 IRR57 pKa = 11.84 RR58 pKa = 11.84 KK59 pKa = 9.6 RR60 pKa = 11.84 QEE62 pKa = 4.03 LLRR65 pKa = 11.84 SIYY68 pKa = 10.89
Molecular weight: 8.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.692
IPC_protein 10.716
Toseland 11.052
ProMoST 11.052
Dawson 11.096
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.213
Grimsley 11.125
Solomon 11.359
Lehninger 11.301
Nozaki 11.038
DTASelect 10.877
Thurlkill 11.038
EMBOSS 11.491
Sillero 11.052
Patrickios 10.979
IPC_peptide 11.374
IPC2_peptide 9.911
IPC2.peptide.svr19 8.865
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
242
0
242
49627
19
1305
205.1
23.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.02 ± 0.205
1.159 ± 0.067
6.222 ± 0.116
6.777 ± 0.202
4.504 ± 0.131
6.319 ± 0.204
2.019 ± 0.094
7.115 ± 0.136
6.839 ± 0.231
7.216 ± 0.129
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.974 ± 0.102
5.541 ± 0.148
3.577 ± 0.1
3.413 ± 0.102
4.802 ± 0.11
6.66 ± 0.174
5.767 ± 0.237
6.613 ± 0.127
1.427 ± 0.07
4.036 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here