Streptomyces sp. Mg1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7098 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4V634|B4V634_9ACTN Ribulose-phosphate 3-epimerase OS=Streptomyces sp. Mg1 OX=465541 GN=rpe PE=3 SV=1
MM1 pKa = 7.84PPTAPDD7 pKa = 3.61YY8 pKa = 11.12ARR10 pKa = 11.84HH11 pKa = 5.24FTPGHH16 pKa = 4.92TFSYY20 pKa = 10.7EE21 pKa = 3.91DD22 pKa = 4.21GPVGTLSVVDD32 pKa = 4.52AGDD35 pKa = 3.34LRR37 pKa = 11.84LPSGSVVACDD47 pKa = 3.96PFICLGTGDD56 pKa = 4.66IPPFTARR63 pKa = 11.84VAPGSYY69 pKa = 8.81RR70 pKa = 11.84VEE72 pKa = 3.96AAVVTITRR80 pKa = 11.84PEE82 pKa = 3.96EE83 pKa = 3.92PPARR87 pKa = 11.84DD88 pKa = 3.09PHH90 pKa = 7.79LRR92 pKa = 11.84IAAVRR97 pKa = 11.84LVIADD102 pKa = 4.43RR103 pKa = 11.84PAVTWEE109 pKa = 3.92PALHH113 pKa = 6.63EE114 pKa = 4.72GQDD117 pKa = 3.62PATLDD122 pKa = 3.35EE123 pKa = 5.67DD124 pKa = 4.06EE125 pKa = 4.54FFGYY129 pKa = 10.49GVDD132 pKa = 3.55AGTGCFYY139 pKa = 11.14DD140 pKa = 4.11AAADD144 pKa = 4.61DD145 pKa = 4.68SFPDD149 pKa = 3.81CEE151 pKa = 4.43GDD153 pKa = 5.47EE154 pKa = 4.63GPLWDD159 pKa = 4.07AFEE162 pKa = 4.61ATGHH166 pKa = 5.42QPGPYY171 pKa = 9.95LVDD174 pKa = 4.23GEE176 pKa = 4.95DD177 pKa = 3.41GHH179 pKa = 7.63NLVAFGSGWGDD190 pKa = 3.04GAYY193 pKa = 6.63PTWVGRR199 pKa = 11.84DD200 pKa = 3.3AEE202 pKa = 4.45GEE204 pKa = 4.14ITCFVTDD211 pKa = 4.05FFVVPPSEE219 pKa = 4.66DD220 pKa = 3.19LPAA223 pKa = 5.89

Molecular weight: 23.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4VAD2|B4VAD2_9ACTN Magnesium transport protein CorA OS=Streptomyces sp. Mg1 OX=465541 GN=corA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.34GRR40 pKa = 11.84AALSAA45 pKa = 3.96

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7098

0

7098

2321781

29

11478

327.1

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.267 ± 0.058

0.791 ± 0.008

5.718 ± 0.023

5.762 ± 0.028

2.739 ± 0.014

9.65 ± 0.032

2.258 ± 0.016

3.134 ± 0.021

2.135 ± 0.028

10.452 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.752 ± 0.012

1.726 ± 0.018

6.182 ± 0.029

2.718 ± 0.015

7.957 ± 0.036

4.993 ± 0.019

5.939 ± 0.029

8.283 ± 0.029

1.508 ± 0.012

2.036 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski