Amino acid dipepetide frequency for Streptomyces sp. Mg1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.86AlaAla: 23.86 ± 0.175
1.106AlaCys: 1.106 ± 0.025
8.344AlaAsp: 8.344 ± 0.068
9.19AlaGlu: 9.19 ± 0.101
3.73AlaPhe: 3.73 ± 0.049
14.545AlaGly: 14.545 ± 0.115
2.969AlaHis: 2.969 ± 0.039
3.458AlaIle: 3.458 ± 0.045
2.957AlaLys: 2.957 ± 0.044
15.34AlaLeu: 15.34 ± 0.119
2.661AlaMet: 2.661 ± 0.034
1.939AlaAsn: 1.939 ± 0.032
7.83AlaPro: 7.83 ± 0.093
3.89AlaGln: 3.89 ± 0.048
10.561AlaArg: 10.561 ± 0.088
5.914AlaSer: 5.914 ± 0.057
7.091AlaThr: 7.091 ± 0.063
12.583AlaVal: 12.583 ± 0.088
1.964AlaTrp: 1.964 ± 0.031
2.734AlaTyr: 2.734 ± 0.033
0.0AlaXaa: 0.0 ± 0.0
Cys
1.175CysAla: 1.175 ± 0.026
0.088CysCys: 0.088 ± 0.007
0.477CysAsp: 0.477 ± 0.015
0.413CysGlu: 0.413 ± 0.013
0.221CysPhe: 0.221 ± 0.01
0.963CysGly: 0.963 ± 0.022
0.19CysHis: 0.19 ± 0.009
0.155CysIle: 0.155 ± 0.009
0.128CysLys: 0.128 ± 0.009
0.758CysLeu: 0.758 ± 0.019
0.131CysMet: 0.131 ± 0.008
0.135CysAsn: 0.135 ± 0.008
0.475CysPro: 0.475 ± 0.017
0.176CysGln: 0.176 ± 0.009
0.584CysArg: 0.584 ± 0.016
0.401CysSer: 0.401 ± 0.014
0.478CysThr: 0.478 ± 0.015
0.684CysVal: 0.684 ± 0.016
0.128CysTrp: 0.128 ± 0.008
0.15CysTyr: 0.15 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.61AspAla: 7.61 ± 0.092
0.431AspCys: 0.431 ± 0.013
2.963AspAsp: 2.963 ± 0.038
3.637AspGlu: 3.637 ± 0.049
1.569AspPhe: 1.569 ± 0.026
6.135AspGly: 6.135 ± 0.065
1.401AspHis: 1.401 ± 0.028
1.771AspIle: 1.771 ± 0.032
1.159AspLys: 1.159 ± 0.027
6.059AspLeu: 6.059 ± 0.056
0.784AspMet: 0.784 ± 0.017
0.882AspAsn: 0.882 ± 0.02
4.599AspPro: 4.599 ± 0.051
1.491AspGln: 1.491 ± 0.029
4.758AspArg: 4.758 ± 0.048
2.46AspSer: 2.46 ± 0.033
3.108AspThr: 3.108 ± 0.04
4.282AspVal: 4.282 ± 0.04
1.012AspTrp: 1.012 ± 0.022
1.066AspTyr: 1.066 ± 0.022
0.0AspXaa: 0.0 ± 0.0
Glu
7.94GluAla: 7.94 ± 0.094
0.388GluCys: 0.388 ± 0.016
2.832GluAsp: 2.832 ± 0.037
3.63GluGlu: 3.63 ± 0.048
1.543GluPhe: 1.543 ± 0.028
4.542GluGly: 4.542 ± 0.051
1.471GluHis: 1.471 ± 0.026
2.255GluIle: 2.255 ± 0.036
1.337GluLys: 1.337 ± 0.03
7.03GluLeu: 7.03 ± 0.073
0.891GluMet: 0.891 ± 0.021
1.022GluAsn: 1.022 ± 0.021
3.346GluPro: 3.346 ± 0.047
2.167GluGln: 2.167 ± 0.036
5.495GluArg: 5.495 ± 0.057
2.562GluSer: 2.562 ± 0.041
2.791GluThr: 2.791 ± 0.035
4.513GluVal: 4.513 ± 0.055
0.754GluTrp: 0.754 ± 0.015
1.111GluTyr: 1.111 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
3.765PheAla: 3.765 ± 0.045
0.268PheCys: 0.268 ± 0.011
1.888PheAsp: 1.888 ± 0.028
1.472PheGlu: 1.472 ± 0.026
0.851PhePhe: 0.851 ± 0.023
3.072PheGly: 3.072 ± 0.036
0.603PheHis: 0.603 ± 0.016
0.751PheIle: 0.751 ± 0.019
0.541PheLys: 0.541 ± 0.016
2.647PheLeu: 2.647 ± 0.037
0.405PheMet: 0.405 ± 0.015
0.547PheAsn: 0.547 ± 0.017
1.427PhePro: 1.427 ± 0.027
0.672PheGln: 0.672 ± 0.019
1.825PheArg: 1.825 ± 0.033
1.516PheSer: 1.516 ± 0.026
2.021PheThr: 2.021 ± 0.03
2.135PheVal: 2.135 ± 0.032
0.42PheTrp: 0.42 ± 0.016
0.562PheTyr: 0.562 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
12.193GlyAla: 12.193 ± 0.093
0.852GlyCys: 0.852 ± 0.021
5.016GlyAsp: 5.016 ± 0.052
5.232GlyGlu: 5.232 ± 0.056
2.885GlyPhe: 2.885 ± 0.042
9.167GlyGly: 9.167 ± 0.091
2.381GlyHis: 2.381 ± 0.03
3.611GlyIle: 3.611 ± 0.043
2.473GlyLys: 2.473 ± 0.042
9.764GlyLeu: 9.764 ± 0.065
2.026GlyMet: 2.026 ± 0.029
1.755GlyAsn: 1.755 ± 0.033
5.513GlyPro: 5.513 ± 0.06
2.713GlyGln: 2.713 ± 0.039
7.908GlyArg: 7.908 ± 0.065
5.543GlySer: 5.543 ± 0.058
5.997GlyThr: 5.997 ± 0.07
7.504GlyVal: 7.504 ± 0.073
1.722GlyTrp: 1.722 ± 0.026
2.243GlyTyr: 2.243 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
2.657HisAla: 2.657 ± 0.036
0.202HisCys: 0.202 ± 0.011
1.245HisAsp: 1.245 ± 0.025
1.2HisGlu: 1.2 ± 0.022
0.627HisPhe: 0.627 ± 0.018
2.347HisGly: 2.347 ± 0.031
0.69HisHis: 0.69 ± 0.018
0.639HisIle: 0.639 ± 0.016
0.358HisLys: 0.358 ± 0.015
2.421HisLeu: 2.421 ± 0.032
0.376HisMet: 0.376 ± 0.013
0.351HisAsn: 0.351 ± 0.012
1.889HisPro: 1.889 ± 0.03
0.643HisGln: 0.643 ± 0.017
2.029HisArg: 2.029 ± 0.036
1.041HisSer: 1.041 ± 0.023
1.363HisThr: 1.363 ± 0.028
1.678HisVal: 1.678 ± 0.031
0.373HisTrp: 0.373 ± 0.014
0.455HisTyr: 0.455 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.933IleAla: 4.933 ± 0.051
0.305IleCys: 0.305 ± 0.011
2.143IleAsp: 2.143 ± 0.036
2.026IleGlu: 2.026 ± 0.036
0.655IlePhe: 0.655 ± 0.019
3.645IleGly: 3.645 ± 0.042
0.625IleHis: 0.625 ± 0.016
0.819IleIle: 0.819 ± 0.022
0.715IleLys: 0.715 ± 0.021
2.359IleLeu: 2.359 ± 0.038
0.424IleMet: 0.424 ± 0.013
0.672IleAsn: 0.672 ± 0.019
1.743IlePro: 1.743 ± 0.03
0.743IleGln: 0.743 ± 0.019
2.274IleArg: 2.274 ± 0.028
1.687IleSer: 1.687 ± 0.025
2.203IleThr: 2.203 ± 0.036
2.56IleVal: 2.56 ± 0.035
0.346IleTrp: 0.346 ± 0.014
0.465IleTyr: 0.465 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
3.03LysAla: 3.03 ± 0.05
0.107LysCys: 0.107 ± 0.008
1.293LysAsp: 1.293 ± 0.029
1.197LysGlu: 1.197 ± 0.024
0.467LysPhe: 0.467 ± 0.016
1.828LysGly: 1.828 ± 0.032
0.408LysHis: 0.408 ± 0.014
0.883LysIle: 0.883 ± 0.021
0.814LysLys: 0.814 ± 0.026
1.976LysLeu: 1.976 ± 0.032
0.395LysMet: 0.395 ± 0.012
0.539LysAsn: 0.539 ± 0.016
1.354LysPro: 1.354 ± 0.03
0.669LysGln: 0.669 ± 0.02
1.401LysArg: 1.401 ± 0.027
1.109LysSer: 1.109 ± 0.023
1.333LysThr: 1.333 ± 0.033
1.851LysVal: 1.851 ± 0.034
0.252LysTrp: 0.252 ± 0.011
0.447LysTyr: 0.447 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
15.929LeuAla: 15.929 ± 0.117
0.856LeuCys: 0.856 ± 0.022
6.639LeuAsp: 6.639 ± 0.054
4.862LeuGlu: 4.862 ± 0.055
2.676LeuPhe: 2.676 ± 0.044
9.546LeuGly: 9.546 ± 0.068
2.297LeuHis: 2.297 ± 0.039
3.319LeuIle: 3.319 ± 0.046
1.955LeuLys: 1.955 ± 0.031
11.542LeuLeu: 11.542 ± 0.113
1.642LeuMet: 1.642 ± 0.026
1.631LeuAsn: 1.631 ± 0.031
6.581LeuPro: 6.581 ± 0.061
2.147LeuGln: 2.147 ± 0.034
8.713LeuArg: 8.713 ± 0.085
5.335LeuSer: 5.335 ± 0.046
6.876LeuThr: 6.876 ± 0.065
8.771LeuVal: 8.771 ± 0.067
1.317LeuTrp: 1.317 ± 0.026
1.886LeuTyr: 1.886 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.357MetAla: 2.357 ± 0.03
0.128MetCys: 0.128 ± 0.008
0.937MetAsp: 0.937 ± 0.02
0.769MetGlu: 0.769 ± 0.019
0.433MetPhe: 0.433 ± 0.016
1.333MetGly: 1.333 ± 0.027
0.344MetHis: 0.344 ± 0.012
0.671MetIle: 0.671 ± 0.018
0.414MetLys: 0.414 ± 0.013
1.739MetLeu: 1.739 ± 0.027
0.296MetMet: 0.296 ± 0.01
0.449MetAsn: 0.449 ± 0.014
1.122MetPro: 1.122 ± 0.024
0.428MetGln: 0.428 ± 0.014
1.458MetArg: 1.458 ± 0.025
1.341MetSer: 1.341 ± 0.023
1.545MetThr: 1.545 ± 0.028
1.235MetVal: 1.235 ± 0.024
0.216MetTrp: 0.216 ± 0.01
0.301MetTyr: 0.301 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.225AsnAla: 2.225 ± 0.034
0.166AsnCys: 0.166 ± 0.009
0.918AsnAsp: 0.918 ± 0.023
0.802AsnGlu: 0.802 ± 0.023
0.475AsnPhe: 0.475 ± 0.015
1.781AsnGly: 1.781 ± 0.036
0.369AsnHis: 0.369 ± 0.013
0.613AsnIle: 0.613 ± 0.017
0.403AsnLys: 0.403 ± 0.016
1.685AsnLeu: 1.685 ± 0.029
0.297AsnMet: 0.297 ± 0.012
0.408AsnAsn: 0.408 ± 0.015
1.363AsnPro: 1.363 ± 0.028
0.52AsnGln: 0.52 ± 0.017
1.27AsnArg: 1.27 ± 0.024
0.892AsnSer: 0.892 ± 0.021
1.134AsnThr: 1.134 ± 0.027
1.287AsnVal: 1.287 ± 0.024
0.266AsnTrp: 0.266 ± 0.012
0.388AsnTyr: 0.388 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
9.837ProAla: 9.837 ± 0.11
0.323ProCys: 0.323 ± 0.012
4.313ProAsp: 4.313 ± 0.049
4.429ProGlu: 4.429 ± 0.047
1.557ProPhe: 1.557 ± 0.031
7.101ProGly: 7.101 ± 0.081
1.341ProHis: 1.341 ± 0.024
1.197ProIle: 1.197 ± 0.023
1.191ProLys: 1.191 ± 0.03
5.482ProLeu: 5.482 ± 0.05
1.013ProMet: 1.013 ± 0.024
0.897ProAsn: 0.897 ± 0.022
3.464ProPro: 3.464 ± 0.064
1.86ProGln: 1.86 ± 0.028
3.94ProArg: 3.94 ± 0.046
3.018ProSer: 3.018 ± 0.046
2.986ProThr: 2.986 ± 0.038
5.55ProVal: 5.55 ± 0.05
0.939ProTrp: 0.939 ± 0.021
1.385ProTyr: 1.385 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.88GlnAla: 3.88 ± 0.052
0.169GlnCys: 0.169 ± 0.008
1.485GlnAsp: 1.485 ± 0.024
1.453GlnGlu: 1.453 ± 0.027
0.65GlnPhe: 0.65 ± 0.015
2.322GlnGly: 2.322 ± 0.038
0.618GlnHis: 0.618 ± 0.016
1.051GlnIle: 1.051 ± 0.022
0.587GlnLys: 0.587 ± 0.017
2.952GlnLeu: 2.952 ± 0.039
0.483GlnMet: 0.483 ± 0.015
0.497GlnAsn: 0.497 ± 0.017
1.645GlnPro: 1.645 ± 0.028
1.175GlnGln: 1.175 ± 0.035
2.299GlnArg: 2.299 ± 0.033
1.248GlnSer: 1.248 ± 0.024
1.314GlnThr: 1.314 ± 0.024
2.288GlnVal: 2.288 ± 0.034
0.472GlnTrp: 0.472 ± 0.015
0.591GlnTyr: 0.591 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
10.18ArgAla: 10.18 ± 0.1
0.575ArgCys: 0.575 ± 0.015
4.097ArgAsp: 4.097 ± 0.051
4.892ArgGlu: 4.892 ± 0.057
2.381ArgPhe: 2.381 ± 0.036
5.964ArgGly: 5.964 ± 0.053
2.067ArgHis: 2.067 ± 0.035
3.25ArgIle: 3.25 ± 0.044
1.597ArgLys: 1.597 ± 0.033
8.807ArgLeu: 8.807 ± 0.078
1.718ArgMet: 1.718 ± 0.025
1.298ArgAsn: 1.298 ± 0.026
5.019ArgPro: 5.019 ± 0.055
2.207ArgGln: 2.207 ± 0.028
7.486ArgArg: 7.486 ± 0.076
3.936ArgSer: 3.936 ± 0.047
5.201ArgThr: 5.201 ± 0.051
5.756ArgVal: 5.756 ± 0.056
1.359ArgTrp: 1.359 ± 0.024
1.776ArgTyr: 1.776 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
6.955SerAla: 6.955 ± 0.062
0.42SerCys: 0.42 ± 0.015
2.539SerAsp: 2.539 ± 0.035
2.469SerGlu: 2.469 ± 0.033
1.526SerPhe: 1.526 ± 0.026
5.946SerGly: 5.946 ± 0.065
1.006SerHis: 1.006 ± 0.022
1.404SerIle: 1.404 ± 0.025
1.01SerLys: 1.01 ± 0.026
4.917SerLeu: 4.917 ± 0.047
1.053SerMet: 1.053 ± 0.024
0.859SerAsn: 0.859 ± 0.023
3.204SerPro: 3.204 ± 0.037
1.246SerGln: 1.246 ± 0.026
3.575SerArg: 3.575 ± 0.044
2.721SerSer: 2.721 ± 0.048
2.792SerThr: 2.792 ± 0.043
4.176SerVal: 4.176 ± 0.046
0.891SerTrp: 0.891 ± 0.022
1.223SerTyr: 1.223 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
9.019ThrAla: 9.019 ± 0.067
0.424ThrCys: 0.424 ± 0.015
3.29ThrAsp: 3.29 ± 0.044
3.131ThrGlu: 3.131 ± 0.039
1.543ThrPhe: 1.543 ± 0.028
6.761ThrGly: 6.761 ± 0.063
1.179ThrHis: 1.179 ± 0.024
1.572ThrIle: 1.572 ± 0.029
1.131ThrLys: 1.131 ± 0.025
5.685ThrLeu: 5.685 ± 0.053
0.911ThrMet: 0.911 ± 0.023
0.976ThrAsn: 0.976 ± 0.023
4.167ThrPro: 4.167 ± 0.045
1.299ThrGln: 1.299 ± 0.025
3.809ThrArg: 3.809 ± 0.046
2.947ThrSer: 2.947 ± 0.04
3.598ThrThr: 3.598 ± 0.054
5.812ThrVal: 5.812 ± 0.062
0.851ThrTrp: 0.851 ± 0.021
1.29ThrTyr: 1.29 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
10.593ValAla: 10.593 ± 0.088
0.78ValCys: 0.78 ± 0.017
4.587ValAsp: 4.587 ± 0.053
4.718ValGlu: 4.718 ± 0.057
2.447ValPhe: 2.447 ± 0.034
6.141ValGly: 6.141 ± 0.06
1.889ValHis: 1.889 ± 0.028
2.925ValIle: 2.925 ± 0.038
1.799ValLys: 1.799 ± 0.036
9.499ValLeu: 9.499 ± 0.076
1.472ValMet: 1.472 ± 0.03
1.65ValAsn: 1.65 ± 0.03
5.218ValPro: 5.218 ± 0.046
1.945ValGln: 1.945 ± 0.027
7.087ValArg: 7.087 ± 0.066
4.376ValSer: 4.376 ± 0.049
5.452ValThr: 5.452 ± 0.052
7.599ValVal: 7.599 ± 0.077
1.115ValTrp: 1.115 ± 0.024
1.536ValTyr: 1.536 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.712TrpAla: 1.712 ± 0.03
0.15TrpCys: 0.15 ± 0.009
0.806TrpAsp: 0.806 ± 0.02
0.744TrpGlu: 0.744 ± 0.019
0.513TrpPhe: 0.513 ± 0.015
1.089TrpGly: 1.089 ± 0.023
0.367TrpHis: 0.367 ± 0.012
0.569TrpIle: 0.569 ± 0.016
0.379TrpLys: 0.379 ± 0.015
1.774TrpLeu: 1.774 ± 0.029
0.29TrpMet: 0.29 ± 0.011
0.389TrpAsn: 0.389 ± 0.012
0.805TrpPro: 0.805 ± 0.018
0.604TrpGln: 0.604 ± 0.018
1.325TrpArg: 1.325 ± 0.027
0.944TrpSer: 0.944 ± 0.022
0.988TrpThr: 0.988 ± 0.022
0.977TrpVal: 0.977 ± 0.02
0.326TrpTrp: 0.326 ± 0.013
0.333TrpTyr: 0.333 ± 0.011
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.818TyrAla: 2.818 ± 0.03
0.172TyrCys: 0.172 ± 0.009
1.366TyrAsp: 1.366 ± 0.033
1.357TyrGlu: 1.357 ± 0.026
0.637TyrPhe: 0.637 ± 0.019
2.268TyrGly: 2.268 ± 0.033
0.372TyrHis: 0.372 ± 0.013
0.429TyrIle: 0.429 ± 0.016
0.404TyrLys: 0.404 ± 0.013
2.082TyrLeu: 2.082 ± 0.033
0.246TyrMet: 0.246 ± 0.009
0.366TyrAsn: 0.366 ± 0.015
1.059TyrPro: 1.059 ± 0.022
0.583TyrGln: 0.583 ± 0.018
1.768TyrArg: 1.768 ± 0.033
0.941TyrSer: 0.941 ± 0.019
1.124TyrThr: 1.124 ± 0.024
1.589TyrVal: 1.589 ± 0.026
0.359TyrTrp: 0.359 ± 0.014
0.424TyrTyr: 0.424 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7098 proteins (2321781 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski