Ferrovum sp. Z-31

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Ferrovales; Ferrovaceae; Ferrovum; unclassified Ferrovum

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2451 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A149VYT0|A0A149VYT0_9PROT Probable Fe(2+)-trafficking protein OS=Ferrovum sp. Z-31 OX=1789004 GN=FEMY_11090 PE=3 SV=1
MM1 pKa = 7.64SINMSTQGVEE11 pKa = 3.53IARR14 pKa = 11.84YY15 pKa = 8.5AGAMYY20 pKa = 10.89GLVLDD25 pKa = 4.82DD26 pKa = 3.74ATVVSVEE33 pKa = 3.83NAANAGGSSLNAVMNQVYY51 pKa = 9.87AADD54 pKa = 4.48FSSISNATVATTVVTNLGLTGSLQSQAQAYY84 pKa = 9.69VLAQLNAAPAGSQGATIMTILNMFGQMTSDD114 pKa = 4.05PVWGAAATAWEE125 pKa = 4.39NKK127 pKa = 8.67VSEE130 pKa = 4.43SVTYY134 pKa = 9.73GQNKK138 pKa = 9.63ANVANSSIGGMSPTPVGGTYY158 pKa = 11.08DD159 pKa = 3.5LTTGVDD165 pKa = 3.69TLSGGPNATFIADD178 pKa = 3.52NTGTKK183 pKa = 8.91TLSAADD189 pKa = 4.32TIAATGTGNTLKK201 pKa = 10.74VYY203 pKa = 10.45LAAADD208 pKa = 3.86TTTGGTAGNITGVQNLYY225 pKa = 10.16INHH228 pKa = 7.1AGATAALTQDD238 pKa = 4.18FSTSSFTSITVDD250 pKa = 3.29SEE252 pKa = 4.65AFGAAALTLKK262 pKa = 10.54GQALTLEE269 pKa = 4.24NTGYY273 pKa = 10.9GATITDD279 pKa = 3.76TTDD282 pKa = 2.56TSLTVTVSAMSAGTLTTTGASKK304 pKa = 9.68ATTLNLVSSGTITGGNVVTLSTNAIDD330 pKa = 3.44TALNVSGATAITVTAGITGSADD352 pKa = 3.12LTSITDD358 pKa = 3.28TGTGGNTFDD367 pKa = 4.49ISTAIANAAFTFTGGSGGDD386 pKa = 3.69TLILAAGDD394 pKa = 3.91LTTLTSGSQLNGGGSASAPATLEE417 pKa = 3.98VNDD420 pKa = 4.52TSFSTAAYY428 pKa = 6.49TALNATTNFQILDD441 pKa = 3.77LNAAAGTTINASLITAGFHH460 pKa = 4.24NHH462 pKa = 5.51FAISAGSTNTISNMADD478 pKa = 2.89ASTVDD483 pKa = 3.25ISSAATSDD491 pKa = 3.5VLGGVVGAHH500 pKa = 5.47TLNLNLQSGAATMTEE515 pKa = 3.91GGITVTGLTTINLTSNTSTAGDD537 pKa = 4.0TNVVTAFVNSDD548 pKa = 2.76NTTFNVTGSAALTMAVAAATTTGDD572 pKa = 4.06TINASAFTGAFTLTATSGKK591 pKa = 10.49GDD593 pKa = 3.85IISTGSGTTSITDD606 pKa = 3.46TASATGNTDD615 pKa = 3.07TLLAGHH621 pKa = 6.3TAIDD625 pKa = 4.41TINTTANLPPAATTYY640 pKa = 9.1TATTLTAAMDD650 pKa = 3.76QISNFNIGATASDD663 pKa = 3.84ILKK666 pKa = 9.41MDD668 pKa = 4.09NGTKK672 pKa = 10.37AVGVSADD679 pKa = 4.48LGGTWTVTNGIATTSGTNTAAAFIAAVDD707 pKa = 3.86AATGTAGDD715 pKa = 3.75VVAYY719 pKa = 9.31TNGTNTYY726 pKa = 8.1VAAMDD731 pKa = 3.96GVVGKK736 pKa = 10.3AYY738 pKa = 10.11VVEE741 pKa = 4.03LVGVHH746 pKa = 5.78TATAVGITAAANTIHH761 pKa = 7.11IAA763 pKa = 3.36

Molecular weight: 74.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A149VV54|A0A149VV54_9PROT Antitoxin DinJ OS=Ferrovum sp. Z-31 OX=1789004 GN=dinJ_2 PE=3 SV=1
MM1 pKa = 7.64ARR3 pKa = 11.84NCLPCSRR10 pKa = 11.84SMAQFPDD17 pKa = 3.42AEE19 pKa = 4.62TYY21 pKa = 10.68SPGEE25 pKa = 3.82IMGVCPIRR33 pKa = 11.84VTSSRR38 pKa = 11.84SPLTLMRR45 pKa = 11.84RR46 pKa = 11.84TQKK49 pKa = 10.47PFSALWKK56 pKa = 7.9VTRR59 pKa = 11.84STRR62 pKa = 11.84PARR65 pKa = 11.84RR66 pKa = 11.84SCSLVVSCGILGFSMGGVAEE86 pKa = 4.31KK87 pKa = 10.97YY88 pKa = 9.31EE89 pKa = 4.2RR90 pKa = 11.84TVSGAEE96 pKa = 3.68YY97 pKa = 10.8RR98 pKa = 11.84LNQWNNSSDD107 pKa = 3.47PQHH110 pKa = 6.45FLNFLPEE117 pKa = 4.14PHH119 pKa = 6.25GHH121 pKa = 6.08GSLRR125 pKa = 11.84PTLASLRR132 pKa = 11.84RR133 pKa = 11.84KK134 pKa = 9.99GCVSSSFEE142 pKa = 3.74VRR144 pKa = 11.84IFPIRR149 pKa = 11.84DD150 pKa = 3.29SIACMRR156 pKa = 11.84RR157 pKa = 11.84GSKK160 pKa = 10.09

Molecular weight: 17.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2451

0

2451

734706

29

2891

299.8

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.455 ± 0.051

1.003 ± 0.018

4.949 ± 0.034

6.022 ± 0.051

3.795 ± 0.031

7.759 ± 0.053

2.772 ± 0.025

5.065 ± 0.033

3.544 ± 0.04

11.562 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.023

2.996 ± 0.036

5.124 ± 0.031

4.518 ± 0.033

6.642 ± 0.047

5.801 ± 0.037

5.422 ± 0.037

7.158 ± 0.046

1.548 ± 0.024

2.425 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski