Amino acid dipepetide frequency for Ferrovum sp. Z-31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.083AlaAla: 9.083 ± 0.131
1.111AlaCys: 1.111 ± 0.041
4.304AlaAsp: 4.304 ± 0.079
5.775AlaGlu: 5.775 ± 0.093
3.181AlaPhe: 3.181 ± 0.07
7.406AlaGly: 7.406 ± 0.114
2.683AlaHis: 2.683 ± 0.062
4.496AlaIle: 4.496 ± 0.086
2.921AlaLys: 2.921 ± 0.074
12.858AlaLeu: 12.858 ± 0.155
2.435AlaMet: 2.435 ± 0.061
2.472AlaAsn: 2.472 ± 0.063
4.033AlaPro: 4.033 ± 0.085
5.085AlaGln: 5.085 ± 0.109
6.931AlaArg: 6.931 ± 0.128
4.886AlaSer: 4.886 ± 0.085
4.817AlaThr: 4.817 ± 0.092
6.528AlaVal: 6.528 ± 0.093
1.469AlaTrp: 1.469 ± 0.055
2.078AlaTyr: 2.078 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
1.013CysAla: 1.013 ± 0.045
0.121CysCys: 0.121 ± 0.012
0.475CysAsp: 0.475 ± 0.025
0.478CysGlu: 0.478 ± 0.024
0.389CysPhe: 0.389 ± 0.026
0.949CysGly: 0.949 ± 0.037
0.393CysHis: 0.393 ± 0.024
0.461CysIle: 0.461 ± 0.028
0.23CysLys: 0.23 ± 0.02
1.096CysLeu: 1.096 ± 0.039
0.192CysMet: 0.192 ± 0.016
0.287CysAsn: 0.287 ± 0.022
0.625CysPro: 0.625 ± 0.031
0.46CysGln: 0.46 ± 0.023
0.738CysArg: 0.738 ± 0.033
0.566CysSer: 0.566 ± 0.032
0.491CysThr: 0.491 ± 0.026
0.653CysVal: 0.653 ± 0.03
0.148CysTrp: 0.148 ± 0.012
0.26CysTyr: 0.26 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
4.39AspAla: 4.39 ± 0.09
0.553AspCys: 0.553 ± 0.026
2.261AspAsp: 2.261 ± 0.06
3.105AspGlu: 3.105 ± 0.073
2.213AspPhe: 2.213 ± 0.056
3.381AspGly: 3.381 ± 0.077
1.455AspHis: 1.455 ± 0.05
2.741AspIle: 2.741 ± 0.067
1.716AspLys: 1.716 ± 0.052
6.021AspLeu: 6.021 ± 0.103
1.186AspMet: 1.186 ± 0.044
1.322AspAsn: 1.322 ± 0.04
3.102AspPro: 3.102 ± 0.072
2.292AspGln: 2.292 ± 0.061
3.242AspArg: 3.242 ± 0.072
2.544AspSer: 2.544 ± 0.064
2.491AspThr: 2.491 ± 0.062
2.945AspVal: 2.945 ± 0.071
0.95AspTrp: 0.95 ± 0.033
1.584AspTyr: 1.584 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
6.118GluAla: 6.118 ± 0.108
0.528GluCys: 0.528 ± 0.027
2.688GluAsp: 2.688 ± 0.057
3.845GluGlu: 3.845 ± 0.081
2.116GluPhe: 2.116 ± 0.055
4.618GluGly: 4.618 ± 0.104
1.35GluHis: 1.35 ± 0.044
3.629GluIle: 3.629 ± 0.085
2.733GluLys: 2.733 ± 0.071
5.902GluLeu: 5.902 ± 0.093
1.598GluMet: 1.598 ± 0.043
2.013GluAsn: 2.013 ± 0.059
2.234GluPro: 2.234 ± 0.061
2.796GluGln: 2.796 ± 0.062
4.694GluArg: 4.694 ± 0.094
3.313GluSer: 3.313 ± 0.057
3.265GluThr: 3.265 ± 0.072
4.667GluVal: 4.667 ± 0.095
0.851GluTrp: 0.851 ± 0.033
1.262GluTyr: 1.262 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.077PheAla: 3.077 ± 0.066
0.49PheCys: 0.49 ± 0.027
2.307PheAsp: 2.307 ± 0.063
2.221PheGlu: 2.221 ± 0.057
1.659PhePhe: 1.659 ± 0.059
3.058PheGly: 3.058 ± 0.06
1.068PheHis: 1.068 ± 0.037
1.919PheIle: 1.919 ± 0.054
1.23PheLys: 1.23 ± 0.041
4.185PheLeu: 4.185 ± 0.075
0.995PheMet: 0.995 ± 0.041
1.237PheAsn: 1.237 ± 0.041
1.92PhePro: 1.92 ± 0.05
1.444PheGln: 1.444 ± 0.046
2.089PheArg: 2.089 ± 0.06
2.911PheSer: 2.911 ± 0.06
1.955PheThr: 1.955 ± 0.055
2.616PheVal: 2.616 ± 0.063
0.554PheTrp: 0.554 ± 0.029
1.01PheTyr: 1.01 ± 0.04
0.0PheXaa: 0.0 ± 0.0
Gly
6.491GlyAla: 6.491 ± 0.109
0.906GlyCys: 0.906 ± 0.036
3.586GlyAsp: 3.586 ± 0.072
4.543GlyGlu: 4.543 ± 0.077
3.269GlyPhe: 3.269 ± 0.069
5.759GlyGly: 5.759 ± 0.107
2.193GlyHis: 2.193 ± 0.059
4.554GlyIle: 4.554 ± 0.081
3.616GlyLys: 3.616 ± 0.079
8.514GlyLeu: 8.514 ± 0.116
2.318GlyMet: 2.318 ± 0.056
2.367GlyAsn: 2.367 ± 0.068
2.665GlyPro: 2.665 ± 0.07
3.537GlyGln: 3.537 ± 0.072
4.747GlyArg: 4.747 ± 0.092
4.414GlySer: 4.414 ± 0.089
4.127GlyThr: 4.127 ± 0.087
6.211GlyVal: 6.211 ± 0.106
1.384GlyTrp: 1.384 ± 0.047
2.385GlyTyr: 2.385 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
2.571HisAla: 2.571 ± 0.06
0.392HisCys: 0.392 ± 0.026
1.293HisAsp: 1.293 ± 0.046
1.524HisGlu: 1.524 ± 0.047
1.225HisPhe: 1.225 ± 0.041
2.217HisGly: 2.217 ± 0.052
1.063HisHis: 1.063 ± 0.039
1.134HisIle: 1.134 ± 0.038
0.705HisLys: 0.705 ± 0.03
3.385HisLeu: 3.385 ± 0.073
0.543HisMet: 0.543 ± 0.027
0.664HisAsn: 0.664 ± 0.031
2.004HisPro: 2.004 ± 0.052
1.343HisGln: 1.343 ± 0.043
1.877HisArg: 1.877 ± 0.054
1.44HisSer: 1.44 ± 0.045
1.342HisThr: 1.342 ± 0.04
1.478HisVal: 1.478 ± 0.045
0.633HisTrp: 0.633 ± 0.03
0.882HisTyr: 0.882 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
4.837IleAla: 4.837 ± 0.086
0.442IleCys: 0.442 ± 0.024
2.937IleAsp: 2.937 ± 0.068
3.378IleGlu: 3.378 ± 0.069
1.741IlePhe: 1.741 ± 0.056
3.864IleGly: 3.864 ± 0.081
1.504IleHis: 1.504 ± 0.052
2.28IleIle: 2.28 ± 0.072
1.858IleLys: 1.858 ± 0.061
5.782IleLeu: 5.782 ± 0.095
0.977IleMet: 0.977 ± 0.033
1.61IleAsn: 1.61 ± 0.053
3.072IlePro: 3.072 ± 0.065
2.163IleGln: 2.163 ± 0.051
3.442IleArg: 3.442 ± 0.071
3.111IleSer: 3.111 ± 0.075
2.805IleThr: 2.805 ± 0.063
3.101IleVal: 3.101 ± 0.075
0.574IleTrp: 0.574 ± 0.03
1.173IleTyr: 1.173 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
3.267LysAla: 3.267 ± 0.073
0.21LysCys: 0.21 ± 0.017
1.685LysAsp: 1.685 ± 0.057
2.11LysGlu: 2.11 ± 0.067
1.082LysPhe: 1.082 ± 0.04
2.636LysGly: 2.636 ± 0.063
0.75LysHis: 0.75 ± 0.031
2.175LysIle: 2.175 ± 0.056
1.778LysLys: 1.778 ± 0.057
3.316LysLeu: 3.316 ± 0.078
0.969LysMet: 0.969 ± 0.039
1.375LysAsn: 1.375 ± 0.05
1.926LysPro: 1.926 ± 0.055
1.361LysGln: 1.361 ± 0.042
2.317LysArg: 2.317 ± 0.068
2.312LysSer: 2.312 ± 0.061
2.278LysThr: 2.278 ± 0.061
2.778LysVal: 2.778 ± 0.064
0.351LysTrp: 0.351 ± 0.023
0.762LysTyr: 0.762 ± 0.033
0.0LysXaa: 0.0 ± 0.0
Leu
11.749LeuAla: 11.749 ± 0.148
1.187LeuCys: 1.187 ± 0.05
6.049LeuAsp: 6.049 ± 0.106
7.129LeuGlu: 7.129 ± 0.114
4.128LeuPhe: 4.128 ± 0.085
8.934LeuGly: 8.934 ± 0.118
3.033LeuHis: 3.033 ± 0.063
5.662LeuIle: 5.662 ± 0.097
4.311LeuLys: 4.311 ± 0.093
12.273LeuLeu: 12.273 ± 0.174
2.762LeuMet: 2.762 ± 0.061
3.582LeuAsn: 3.582 ± 0.067
6.415LeuPro: 6.415 ± 0.103
4.56LeuGln: 4.56 ± 0.073
7.46LeuArg: 7.46 ± 0.117
7.417LeuSer: 7.417 ± 0.105
6.974LeuThr: 6.974 ± 0.119
7.761LeuVal: 7.761 ± 0.117
1.779LeuTrp: 1.779 ± 0.049
2.457LeuTyr: 2.457 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
2.719MetAla: 2.719 ± 0.063
0.158MetCys: 0.158 ± 0.013
1.307MetAsp: 1.307 ± 0.037
1.497MetGlu: 1.497 ± 0.042
0.588MetPhe: 0.588 ± 0.028
2.089MetGly: 2.089 ± 0.059
0.497MetHis: 0.497 ± 0.029
1.23MetIle: 1.23 ± 0.044
1.221MetLys: 1.221 ± 0.047
2.255MetLeu: 2.255 ± 0.061
0.64MetMet: 0.64 ± 0.03
1.047MetAsn: 1.047 ± 0.042
1.23MetPro: 1.23 ± 0.039
0.919MetGln: 0.919 ± 0.033
1.494MetArg: 1.494 ± 0.041
1.519MetSer: 1.519 ± 0.044
1.697MetThr: 1.697 ± 0.045
1.812MetVal: 1.812 ± 0.055
0.142MetTrp: 0.142 ± 0.014
0.338MetTyr: 0.338 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.747AsnAla: 2.747 ± 0.067
0.304AsnCys: 0.304 ± 0.02
1.465AsnAsp: 1.465 ± 0.047
1.561AsnGlu: 1.561 ± 0.048
1.322AsnPhe: 1.322 ± 0.048
2.356AsnGly: 2.356 ± 0.06
0.804AsnHis: 0.804 ± 0.035
1.587AsnIle: 1.587 ± 0.051
0.911AsnLys: 0.911 ± 0.04
3.487AsnLeu: 3.487 ± 0.065
0.604AsnMet: 0.604 ± 0.026
0.872AsnAsn: 0.872 ± 0.041
2.213AsnPro: 2.213 ± 0.062
1.418AsnGln: 1.418 ± 0.054
1.948AsnArg: 1.948 ± 0.053
1.671AsnSer: 1.671 ± 0.056
1.594AsnThr: 1.594 ± 0.056
1.737AsnVal: 1.737 ± 0.044
0.476AsnTrp: 0.476 ± 0.026
0.883AsnTyr: 0.883 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
4.776ProAla: 4.776 ± 0.083
0.476ProCys: 0.476 ± 0.026
3.035ProAsp: 3.035 ± 0.072
3.882ProGlu: 3.882 ± 0.07
1.959ProPhe: 1.959 ± 0.052
3.978ProGly: 3.978 ± 0.077
1.454ProHis: 1.454 ± 0.055
2.144ProIle: 2.144 ± 0.053
1.531ProLys: 1.531 ± 0.048
6.057ProLeu: 6.057 ± 0.097
1.288ProMet: 1.288 ± 0.044
1.471ProAsn: 1.471 ± 0.051
2.677ProPro: 2.677 ± 0.072
2.125ProGln: 2.125 ± 0.046
2.675ProArg: 2.675 ± 0.066
2.816ProSer: 2.816 ± 0.051
2.492ProThr: 2.492 ± 0.057
4.166ProVal: 4.166 ± 0.076
0.911ProTrp: 0.911 ± 0.037
1.328ProTyr: 1.328 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
5.048GlnAla: 5.048 ± 0.106
0.366GlnCys: 0.366 ± 0.022
2.066GlnAsp: 2.066 ± 0.05
2.793GlnGlu: 2.793 ± 0.073
1.405GlnPhe: 1.405 ± 0.042
3.905GlnGly: 3.905 ± 0.078
1.2GlnHis: 1.2 ± 0.042
2.379GlnIle: 2.379 ± 0.062
1.661GlnLys: 1.661 ± 0.058
4.324GlnLeu: 4.324 ± 0.075
1.083GlnMet: 1.083 ± 0.038
1.405GlnAsn: 1.405 ± 0.055
1.96GlnPro: 1.96 ± 0.05
2.123GlnGln: 2.123 ± 0.064
3.559GlnArg: 3.559 ± 0.075
2.379GlnSer: 2.379 ± 0.058
2.265GlnThr: 2.265 ± 0.051
3.551GlnVal: 3.551 ± 0.082
0.785GlnTrp: 0.785 ± 0.036
0.923GlnTyr: 0.923 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
6.038ArgAla: 6.038 ± 0.097
0.603ArgCys: 0.603 ± 0.029
3.144ArgAsp: 3.144 ± 0.072
4.086ArgGlu: 4.086 ± 0.086
3.018ArgPhe: 3.018 ± 0.075
4.081ArgGly: 4.081 ± 0.086
2.216ArgHis: 2.216 ± 0.055
3.776ArgIle: 3.776 ± 0.075
2.243ArgLys: 2.243 ± 0.053
7.998ArgLeu: 7.998 ± 0.119
1.696ArgMet: 1.696 ± 0.052
1.994ArgAsn: 1.994 ± 0.048
2.913ArgPro: 2.913 ± 0.067
3.635ArgGln: 3.635 ± 0.076
4.396ArgArg: 4.396 ± 0.076
3.581ArgSer: 3.581 ± 0.076
3.269ArgThr: 3.269 ± 0.077
4.686ArgVal: 4.686 ± 0.088
1.206ArgTrp: 1.206 ± 0.04
1.84ArgTyr: 1.84 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
5.504SerAla: 5.504 ± 0.094
0.581SerCys: 0.581 ± 0.031
2.685SerAsp: 2.685 ± 0.063
3.094SerGlu: 3.094 ± 0.069
2.432SerPhe: 2.432 ± 0.061
5.325SerGly: 5.325 ± 0.09
1.496SerHis: 1.496 ± 0.039
2.631SerIle: 2.631 ± 0.066
1.801SerLys: 1.801 ± 0.057
7.18SerLeu: 7.18 ± 0.098
1.486SerMet: 1.486 ± 0.051
1.633SerAsn: 1.633 ± 0.06
3.186SerPro: 3.186 ± 0.066
2.383SerGln: 2.383 ± 0.061
3.437SerArg: 3.437 ± 0.07
3.744SerSer: 3.744 ± 0.09
3.144SerThr: 3.144 ± 0.079
4.117SerVal: 4.117 ± 0.086
0.832SerTrp: 0.832 ± 0.038
1.318SerTyr: 1.318 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
4.773ThrAla: 4.773 ± 0.084
0.461ThrCys: 0.461 ± 0.027
2.488ThrAsp: 2.488 ± 0.057
2.861ThrGlu: 2.861 ± 0.057
1.899ThrPhe: 1.899 ± 0.055
4.595ThrGly: 4.595 ± 0.098
1.669ThrHis: 1.669 ± 0.051
2.329ThrIle: 2.329 ± 0.063
1.42ThrLys: 1.42 ± 0.049
7.888ThrLeu: 7.888 ± 0.12
0.965ThrMet: 0.965 ± 0.037
1.32ThrAsn: 1.32 ± 0.05
3.475ThrPro: 3.475 ± 0.068
2.449ThrGln: 2.449 ± 0.063
3.529ThrArg: 3.529 ± 0.069
2.831ThrSer: 2.831 ± 0.078
2.989ThrThr: 2.989 ± 0.076
4.266ThrVal: 4.266 ± 0.069
0.765ThrTrp: 0.765 ± 0.033
1.249ThrTyr: 1.249 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
6.917ValAla: 6.917 ± 0.107
0.675ValCys: 0.675 ± 0.032
3.736ValAsp: 3.736 ± 0.079
4.39ValGlu: 4.39 ± 0.082
2.582ValPhe: 2.582 ± 0.065
5.225ValGly: 5.225 ± 0.104
1.767ValHis: 1.767 ± 0.054
3.723ValIle: 3.723 ± 0.073
2.296ValLys: 2.296 ± 0.061
8.232ValLeu: 8.232 ± 0.117
1.84ValMet: 1.84 ± 0.051
2.115ValAsn: 2.115 ± 0.053
3.493ValPro: 3.493 ± 0.08
2.926ValGln: 2.926 ± 0.066
4.754ValArg: 4.754 ± 0.089
4.373ValSer: 4.373 ± 0.081
4.328ValThr: 4.328 ± 0.07
5.781ValVal: 5.781 ± 0.108
0.949ValTrp: 0.949 ± 0.038
1.482ValTyr: 1.482 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
1.22TrpAla: 1.22 ± 0.051
0.167TrpCys: 0.167 ± 0.016
0.668TrpAsp: 0.668 ± 0.032
0.713TrpGlu: 0.713 ± 0.034
0.618TrpPhe: 0.618 ± 0.03
1.113TrpGly: 1.113 ± 0.042
0.46TrpHis: 0.46 ± 0.028
0.887TrpIle: 0.887 ± 0.035
0.64TrpLys: 0.64 ± 0.027
1.978TrpLeu: 1.978 ± 0.053
0.456TrpMet: 0.456 ± 0.026
0.491TrpAsn: 0.491 ± 0.03
0.648TrpPro: 0.648 ± 0.036
0.872TrpGln: 0.872 ± 0.037
1.096TrpArg: 1.096 ± 0.044
0.855TrpSer: 0.855 ± 0.037
0.721TrpThr: 0.721 ± 0.036
1.288TrpVal: 1.288 ± 0.05
0.267TrpTrp: 0.267 ± 0.018
0.325TrpTyr: 0.325 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.212TyrAla: 2.212 ± 0.055
0.294TyrCys: 0.294 ± 0.021
1.313TyrAsp: 1.313 ± 0.041
1.235TyrGlu: 1.235 ± 0.042
1.123TyrPhe: 1.123 ± 0.039
2.097TyrGly: 2.097 ± 0.058
0.661TyrHis: 0.661 ± 0.03
0.916TyrIle: 0.916 ± 0.039
0.617TyrLys: 0.617 ± 0.03
2.891TyrLeu: 2.891 ± 0.072
0.365TyrMet: 0.365 ± 0.022
0.682TyrAsn: 0.682 ± 0.037
1.452TyrPro: 1.452 ± 0.039
1.289TyrGln: 1.289 ± 0.039
1.993TyrArg: 1.993 ± 0.049
1.324TyrSer: 1.324 ± 0.044
1.175TyrThr: 1.175 ± 0.046
1.443TyrVal: 1.443 ± 0.045
0.459TyrTrp: 0.459 ± 0.024
0.71TyrTyr: 0.71 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2451 proteins (734706 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski