Asparagus virus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1B3P0|B1B3P0_9VIRU Triple gene block protein 2 OS=Asparagus virus 3 OX=445435 GN=TGBp2 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 5.13ISYY5 pKa = 10.51IVDD8 pKa = 4.1LLNFLGFTRR17 pKa = 11.84SSRR20 pKa = 11.84PFSLPLVVHH29 pKa = 6.42GVAGSGKK36 pKa = 7.04THH38 pKa = 7.55LLRR41 pKa = 11.84KK42 pKa = 9.73VSLHH46 pKa = 5.51FPEE49 pKa = 5.79LVHH52 pKa = 7.6CSFTPQLIDD61 pKa = 3.67PNSGRR66 pKa = 11.84RR67 pKa = 11.84QLPASEE73 pKa = 4.61TPTDD77 pKa = 4.68LLDD80 pKa = 4.34EE81 pKa = 4.43YY82 pKa = 11.17LGGPNPVVRR91 pKa = 11.84LLKK94 pKa = 10.82VCDD97 pKa = 3.82PLQYY101 pKa = 10.71NCPDD105 pKa = 3.39PEE107 pKa = 4.64IPHH110 pKa = 5.8FQSLTTRR117 pKa = 11.84RR118 pKa = 11.84FCPLTTTLLNSLFGTNIVSAVPTCCRR144 pKa = 11.84IEE146 pKa = 3.98IQDD149 pKa = 4.38PYY151 pKa = 10.33STDD154 pKa = 3.95PIGTVVTFSPEE165 pKa = 3.14IHH167 pKa = 6.36TLLSRR172 pKa = 11.84HH173 pKa = 5.76GCQPTPISEE182 pKa = 4.08LWGLNIRR189 pKa = 11.84VVSCYY194 pKa = 10.34VDD196 pKa = 3.39SLEE199 pKa = 4.54EE200 pKa = 3.69ALLNHH205 pKa = 7.23RR206 pKa = 11.84APLFLALTRR215 pKa = 11.84HH216 pKa = 4.98TAEE219 pKa = 3.64LHH221 pKa = 6.05IFLFDD226 pKa = 3.34ARR228 pKa = 11.84TDD230 pKa = 3.18AAYY233 pKa = 9.98EE234 pKa = 3.95LRR236 pKa = 11.84KK237 pKa = 9.9CLQDD241 pKa = 3.24PRR243 pKa = 11.84HH244 pKa = 6.27RR245 pKa = 11.84GSS247 pKa = 3.03

Molecular weight: 27.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1B3P1|B1B3P1_9VIRU Movement protein TGBp3 OS=Asparagus virus 3 OX=445435 GN=TGBp3 PE=3 SV=1
MM1 pKa = 7.83PGLTPPTNYY10 pKa = 10.27EE11 pKa = 4.2SVFKK15 pKa = 10.64ILAIGALSCLSIYY28 pKa = 8.92SLRR31 pKa = 11.84TNHH34 pKa = 6.73LPHH37 pKa = 6.67TGDD40 pKa = 4.27NIHH43 pKa = 6.69HH44 pKa = 6.93LPHH47 pKa = 6.69GGNYY51 pKa = 10.1ADD53 pKa = 3.56GTKK56 pKa = 9.71RR57 pKa = 11.84VQYY60 pKa = 8.72FRR62 pKa = 11.84PSAPVHH68 pKa = 5.84GSSKK72 pKa = 9.13FTAACAILFLTLLILAQSQWPARR95 pKa = 11.84AVRR98 pKa = 11.84CSVRR102 pKa = 11.84VCGHH106 pKa = 6.28CHH108 pKa = 6.95PDD110 pKa = 2.64STMPSNSDD118 pKa = 2.92RR119 pKa = 3.88

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2295

85

1614

459.0

51.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.235 ± 1.045

1.917 ± 0.806

5.403 ± 0.743

4.88 ± 0.87

4.357 ± 0.19

4.706 ± 0.545

3.66 ± 0.631

5.142 ± 0.244

4.575 ± 1.197

10.109 ± 1.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.539

3.704 ± 0.224

7.625 ± 0.744

3.747 ± 0.355

5.316 ± 0.455

7.277 ± 0.705

7.495 ± 0.322

5.403 ± 1.02

1.046 ± 0.176

3.268 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski