Odonata-associated circular virus 21
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UH63|A0A0B4UH63_9VIRU Putative capsid protein OS=Odonata-associated circular virus 21 OX=1592122 PE=4 SV=1
MM1 pKa = 7.79 EE2 pKa = 6.22 DD3 pKa = 3.32 IIGAGLIGAGGNMVIVSVSEE23 pKa = 4.22 TYY25 pKa = 10.78 DD26 pKa = 4.29 LSTKK30 pKa = 8.62 PNKK33 pKa = 9.36 MSLIALHH40 pKa = 5.93 TPSRR44 pKa = 11.84 EE45 pKa = 4.22 IIKK48 pKa = 8.8 KK49 pKa = 8.08 TYY51 pKa = 9.93 PGLCMNCKK59 pKa = 9.13 YY60 pKa = 10.94 VRR62 pKa = 11.84 IVKK65 pKa = 9.96 QDD67 pKa = 3.09 VTMACASMLPADD79 pKa = 4.36 PLQVGVSSGDD89 pKa = 3.31 IAPQDD94 pKa = 3.6 MFNPILYY101 pKa = 9.69 KK102 pKa = 10.65 AISTEE107 pKa = 3.97 SLSILEE113 pKa = 3.96 ARR115 pKa = 11.84 IVGLQAQPSQNVFNGEE131 pKa = 4.37 SVEE134 pKa = 4.27 KK135 pKa = 10.31 TDD137 pKa = 5.45 DD138 pKa = 3.7 ATNMADD144 pKa = 4.72 DD145 pKa = 3.91 FAVYY149 pKa = 10.15 YY150 pKa = 10.91 ALLSNRR156 pKa = 11.84 DD157 pKa = 3.48 GFRR160 pKa = 11.84 TASPQAGFSMKK171 pKa = 10.54 GLVPLVFEE179 pKa = 4.72 KK180 pKa = 10.92 YY181 pKa = 10.09 YY182 pKa = 10.28 PVGANSSSDD191 pKa = 3.07 GNTAIIRR198 pKa = 11.84 EE199 pKa = 3.99 NSAGNGLEE207 pKa = 3.71 VSTWSGRR214 pKa = 11.84 SMRR217 pKa = 11.84 GRR219 pKa = 11.84 AHH221 pKa = 6.98 PMPRR225 pKa = 11.84 FNTTYY230 pKa = 9.04 VTGVSKK236 pKa = 10.98 AGEE239 pKa = 4.16 TGVNRR244 pKa = 11.84 QEE246 pKa = 4.17 NGMTNGHH253 pKa = 7.36 PYY255 pKa = 9.38 NCQVEE260 pKa = 4.76 MPTIPIIYY268 pKa = 8.61 TGAIIMPPSKK278 pKa = 10.52 LNQLYY283 pKa = 9.24 YY284 pKa = 10.85 RR285 pKa = 11.84 MVVRR289 pKa = 11.84 TYY291 pKa = 10.54 IEE293 pKa = 3.99 FSEE296 pKa = 4.1 VRR298 pKa = 11.84 PIQEE302 pKa = 3.3 ISSFAEE308 pKa = 3.54 LATYY312 pKa = 7.37 YY313 pKa = 10.2 TPEE316 pKa = 4.26 VYY318 pKa = 10.53 GSDD321 pKa = 3.42 YY322 pKa = 10.68 STVAKK327 pKa = 10.25 TMTSQMAMVDD337 pKa = 3.68 TQNADD342 pKa = 2.52 ITKK345 pKa = 10.22 IMDD348 pKa = 3.33 GMM350 pKa = 4.22
Molecular weight: 38.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.03
IPC2_protein 5.219
IPC_protein 5.13
Toseland 5.054
ProMoST 5.258
Dawson 5.105
Bjellqvist 5.245
Wikipedia 4.978
Rodwell 5.029
Grimsley 4.978
Solomon 5.105
Lehninger 5.067
Nozaki 5.232
DTASelect 5.385
Thurlkill 5.054
EMBOSS 5.016
Sillero 5.296
Patrickios 3.605
IPC_peptide 5.118
IPC2_peptide 5.296
IPC2.peptide.svr19 5.307
Protein with the highest isoelectric point:
>tr|A0A0B4UH63|A0A0B4UH63_9VIRU Putative capsid protein OS=Odonata-associated circular virus 21 OX=1592122 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.17 KK3 pKa = 10.41 CEE5 pKa = 4.01 ACEE8 pKa = 3.86 EE9 pKa = 4.19 RR10 pKa = 11.84 PANYY14 pKa = 10.34 GYY16 pKa = 10.78 VKK18 pKa = 10.09 WINCIDD24 pKa = 4.24 GIDD27 pKa = 4.05 RR28 pKa = 11.84 YY29 pKa = 9.67 WFGYY33 pKa = 9.78 ICDD36 pKa = 3.59 EE37 pKa = 4.7 CYY39 pKa = 10.66 HH40 pKa = 6.86 KK41 pKa = 11.02 FEE43 pKa = 4.64 EE44 pKa = 4.61 EE45 pKa = 3.65 WAKK48 pKa = 10.96 EE49 pKa = 4.01 EE50 pKa = 4.15 EE51 pKa = 4.85 CNVQYY56 pKa = 10.66 IGTISAEE63 pKa = 4.07 DD64 pKa = 3.47 WNEE67 pKa = 3.59 DD68 pKa = 3.31 GIIRR72 pKa = 11.84 VFEE75 pKa = 4.2 NLDD78 pKa = 3.27 GHH80 pKa = 5.97 EE81 pKa = 4.38 LYY83 pKa = 10.25 IGRR86 pKa = 11.84 EE87 pKa = 3.76 IGKK90 pKa = 9.23 HH91 pKa = 4.58 GFRR94 pKa = 11.84 HH95 pKa = 5.35 YY96 pKa = 10.14 QFCMDD101 pKa = 4.44 CAGDD105 pKa = 3.94 LEE107 pKa = 6.03 KK108 pKa = 10.77 YY109 pKa = 8.37 TADD112 pKa = 4.29 NRR114 pKa = 11.84 TGWHH118 pKa = 5.16 VEE120 pKa = 3.95 RR121 pKa = 11.84 CISWEE126 pKa = 3.82 MSKK129 pKa = 10.78 GYY131 pKa = 9.57 CRR133 pKa = 11.84 KK134 pKa = 8.5 TGDD137 pKa = 3.11 YY138 pKa = 10.34 RR139 pKa = 11.84 YY140 pKa = 9.98 IGDD143 pKa = 3.75 SRR145 pKa = 11.84 EE146 pKa = 3.58 EE147 pKa = 4.21 RR148 pKa = 11.84 YY149 pKa = 9.81 YY150 pKa = 10.56 AWLRR154 pKa = 11.84 ARR156 pKa = 11.84 RR157 pKa = 11.84 PLPIWRR163 pKa = 11.84 SFGASVVKK171 pKa = 10.89 QNDD174 pKa = 2.95 RR175 pKa = 11.84 SITVWVDD182 pKa = 2.99 TEE184 pKa = 4.33 GKK186 pKa = 9.93 AGKK189 pKa = 8.29 STFSYY194 pKa = 10.12 ILEE197 pKa = 4.16 RR198 pKa = 11.84 RR199 pKa = 11.84 GKK201 pKa = 9.78 CLNIPRR207 pKa = 11.84 TEE209 pKa = 4.15 HH210 pKa = 6.04 NPTRR214 pKa = 11.84 LIDD217 pKa = 3.91 FVAMHH222 pKa = 6.19 YY223 pKa = 10.22 KK224 pKa = 10.51 GEE226 pKa = 4.02 PLIIVDD232 pKa = 4.16 IPRR235 pKa = 11.84 DD236 pKa = 3.51 QKK238 pKa = 11.07 LGKK241 pKa = 8.52 EE242 pKa = 4.06 LCRR245 pKa = 11.84 ALEE248 pKa = 4.28 TIKK251 pKa = 11.17 DD252 pKa = 3.92 GVITSAKK259 pKa = 8.61 YY260 pKa = 9.95 QGTKK264 pKa = 9.38 MFIKK268 pKa = 10.25 GVKK271 pKa = 9.37 ILVFTNHH278 pKa = 6.3 FLDD281 pKa = 3.48 KK282 pKa = 8.73 TTYY285 pKa = 10.68 AALTEE290 pKa = 4.39 DD291 pKa = 3.13 RR292 pKa = 11.84 WDD294 pKa = 3.74 VKK296 pKa = 9.99 SLKK299 pKa = 10.11 ASKK302 pKa = 9.97 SHH304 pKa = 6.41 PRR306 pKa = 3.28
Molecular weight: 35.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.775
IPC2_protein 7.0
IPC_protein 6.942
Toseland 6.693
ProMoST 7.585
Dawson 7.585
Bjellqvist 7.907
Wikipedia 7.512
Rodwell 7.6
Grimsley 6.751
Solomon 7.629
Lehninger 7.658
Nozaki 8.156
DTASelect 7.761
Thurlkill 7.805
EMBOSS 7.834
Sillero 8.112
Patrickios 4.05
IPC_peptide 7.629
IPC2_peptide 7.263
IPC2.peptide.svr19 7.171
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
656
306
350
328.0
37.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.555 ± 0.913
2.439 ± 1.02
5.488 ± 0.721
6.86 ± 1.352
3.049 ± 0.376
7.622 ± 0.073
1.829 ± 0.765
7.47 ± 0.257
5.945 ± 1.531
5.335 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.811 ± 1.498
4.421 ± 0.793
4.268 ± 1.138
2.744 ± 0.764
5.793 ± 1.411
6.707 ± 1.693
6.402 ± 0.808
6.098 ± 1.048
1.677 ± 1.095
5.488 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here