Gordonia phage AnClar

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sourvirus; unclassified Sourvirus

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XML1|A0A6G6XML1_9CAUD Uncharacterized protein OS=Gordonia phage AnClar OX=2704036 GN=73 PE=4 SV=1
MM1 pKa = 6.61TTPEE5 pKa = 4.48FAPSTNRR12 pKa = 11.84GTALLHH18 pKa = 6.53EE19 pKa = 4.6IMALVEE25 pKa = 4.2AEE27 pKa = 4.41VAAEE31 pKa = 3.79AQAQQRR37 pKa = 11.84VADD40 pKa = 3.93WLANDD45 pKa = 3.6SSARR49 pKa = 11.84HH50 pKa = 5.91WDD52 pKa = 3.32QGDD55 pKa = 3.41WLQANLGAVSDD66 pKa = 4.21SVNLVALATADD77 pKa = 4.34LGLPDD82 pKa = 3.91ATALADD88 pKa = 3.71YY89 pKa = 9.99EE90 pKa = 4.82GEE92 pKa = 4.21FVHH95 pKa = 7.36IDD97 pKa = 3.58LPPTVRR103 pKa = 11.84NLCGTAGCFAGHH115 pKa = 6.61AVMAVGDD122 pKa = 4.24MPHH125 pKa = 7.01LSALVGDD132 pKa = 4.23ILGHH136 pKa = 5.12EE137 pKa = 4.73RR138 pKa = 11.84IDD140 pKa = 3.64VSMHH144 pKa = 5.29QVTTYY149 pKa = 9.63DD150 pKa = 3.07TGDD153 pKa = 3.22TLMVEE158 pKa = 4.86DD159 pKa = 5.2RR160 pKa = 11.84AADD163 pKa = 3.81LLGIDD168 pKa = 4.25TDD170 pKa = 3.96TADD173 pKa = 3.89VLFNASNTLADD184 pKa = 3.5LRR186 pKa = 11.84AIVEE190 pKa = 4.62TIDD193 pKa = 3.17RR194 pKa = 11.84HH195 pKa = 6.08GDD197 pKa = 3.48VVEE200 pKa = 4.27SRR202 pKa = 11.84DD203 pKa = 3.39MCADD207 pKa = 3.36CGEE210 pKa = 4.35WTWRR214 pKa = 11.84CAYY217 pKa = 10.41DD218 pKa = 5.21GEE220 pKa = 4.74TCDD223 pKa = 3.76EE224 pKa = 4.37CDD226 pKa = 3.49EE227 pKa = 5.11HH228 pKa = 7.93EE229 pKa = 4.41VDD231 pKa = 5.07CSCSDD236 pKa = 3.38EE237 pKa = 4.36HH238 pKa = 6.64WCDD241 pKa = 2.88RR242 pKa = 11.84CGNATVDD249 pKa = 5.14DD250 pKa = 4.67EE251 pKa = 6.92GDD253 pKa = 3.36MCEE256 pKa = 4.23DD257 pKa = 5.35CEE259 pKa = 5.46TEE261 pKa = 4.49DD262 pKa = 5.01QDD264 pKa = 5.74SSDD267 pKa = 3.89DD268 pKa = 3.53

Molecular weight: 28.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XM99|A0A6G6XM99_9CAUD Ribbon-helix-helix DNA binding domain protein OS=Gordonia phage AnClar OX=2704036 GN=58 PE=4 SV=1
MM1 pKa = 6.92TTTEE5 pKa = 4.2PVSKK9 pKa = 10.66YY10 pKa = 10.1GAYY13 pKa = 10.05DD14 pKa = 3.17VEE16 pKa = 4.38RR17 pKa = 11.84AQRR20 pKa = 11.84VAEE23 pKa = 4.06LTRR26 pKa = 11.84AGLSARR32 pKa = 11.84EE33 pKa = 3.41IAARR37 pKa = 11.84LRR39 pKa = 11.84VTVRR43 pKa = 11.84TVGRR47 pKa = 11.84DD48 pKa = 3.11RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84TGTNGHH57 pKa = 6.45RR58 pKa = 11.84PARR61 pKa = 11.84WSEE64 pKa = 3.95EE65 pKa = 3.43KK66 pKa = 10.52LARR69 pKa = 11.84AKK71 pKa = 10.85ALLDD75 pKa = 4.19DD76 pKa = 4.37GASYY80 pKa = 11.13RR81 pKa = 11.84EE82 pKa = 3.73TAMTVGCDD90 pKa = 3.29PTHH93 pKa = 6.55LRR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84FPGYY101 pKa = 9.94GWPPGHH107 pKa = 6.71WPEE110 pKa = 4.34GLNPQIIAAARR121 pKa = 11.84RR122 pKa = 11.84EE123 pKa = 4.16KK124 pKa = 10.98

Molecular weight: 13.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

19187

49

1828

236.9

25.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.519 ± 0.3

0.98 ± 0.162

6.89 ± 0.295

5.816 ± 0.303

2.371 ± 0.164

8.818 ± 0.423

1.876 ± 0.175

4.352 ± 0.268

2.324 ± 0.178

8.151 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.116

2.752 ± 0.199

5.681 ± 0.268

3.502 ± 0.204

6.947 ± 0.342

5.707 ± 0.212

6.864 ± 0.227

7.886 ± 0.202

2.017 ± 0.095

2.111 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski