Thermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae; Thermoproteus; Thermoproteus tenax

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2047 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4RNE8|G4RNE8_THETK Uncharacterized protein OS=Thermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1) OX=768679 GN=TTX_0421 PE=4 SV=1
MM1 pKa = 7.22LTYY4 pKa = 10.35IFIPIDD10 pKa = 3.13IFFVISIIEE19 pKa = 4.17EE20 pKa = 3.61IKK22 pKa = 10.79RR23 pKa = 11.84RR24 pKa = 11.84VQAYY28 pKa = 9.36GMPCGAPPNGLKK40 pKa = 10.61LEE42 pKa = 4.33EE43 pKa = 4.13NLYY46 pKa = 11.11VSDD49 pKa = 3.17GWAIYY54 pKa = 10.26SSQDD58 pKa = 3.11GTKK61 pKa = 9.38CLYY64 pKa = 9.33MGEE67 pKa = 4.22VAQAVAYY74 pKa = 9.35VGKK77 pKa = 10.1VDD79 pKa = 3.85CVKK82 pKa = 10.66KK83 pKa = 10.65IEE85 pKa = 4.23GVKK88 pKa = 10.49LSPPFLEE95 pKa = 4.97LYY97 pKa = 10.21EE98 pKa = 4.56DD99 pKa = 3.61EE100 pKa = 4.81EE101 pKa = 4.91YY102 pKa = 11.33AVILGVCGTDD112 pKa = 3.09VCIQEE117 pKa = 4.39WRR119 pKa = 11.84DD120 pKa = 3.26EE121 pKa = 4.44APNCTCISNLKK132 pKa = 9.41LDD134 pKa = 4.49EE135 pKa = 4.52YY136 pKa = 10.99IKK138 pKa = 10.09MVKK141 pKa = 9.77ILEE144 pKa = 4.33NYY146 pKa = 10.07NLIDD150 pKa = 3.32

Molecular weight: 16.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4RNY0|G4RNY0_THETK Uncharacterized protein OS=Thermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1) OX=768679 GN=TTX_0614 PE=4 SV=1
MM1 pKa = 7.58ARR3 pKa = 11.84NKK5 pKa = 10.72ALGRR9 pKa = 11.84KK10 pKa = 8.73LRR12 pKa = 11.84LASALKK18 pKa = 10.48SNRR21 pKa = 11.84DD22 pKa = 3.22PPVWVRR28 pKa = 11.84VKK30 pKa = 9.79TKK32 pKa = 10.69NRR34 pKa = 11.84VTRR37 pKa = 11.84SPARR41 pKa = 11.84RR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84VKK48 pKa = 10.82LKK50 pKa = 10.76AA51 pKa = 3.65

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2047

0

2047

553635

28

2663

270.5

29.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.071 ± 0.065

0.814 ± 0.02

4.537 ± 0.039

6.696 ± 0.077

3.433 ± 0.033

7.891 ± 0.056

1.434 ± 0.02

6.234 ± 0.048

4.726 ± 0.049

10.88 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.023

2.579 ± 0.039

5.083 ± 0.043

2.115 ± 0.035

7.091 ± 0.075

5.663 ± 0.049

4.252 ± 0.056

8.892 ± 0.054

1.325 ± 0.023

4.169 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski