Microbacterium phage Pikmin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pikminvirus; Microbacterium virus Pikmin

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1CJG4|A0A2P1CJG4_9CAUD Uncharacterized protein OS=Microbacterium phage Pikmin OX=2099444 GN=7 PE=4 SV=1
MM1 pKa = 6.8ATKK4 pKa = 9.81TIAINISDD12 pKa = 3.95NQGYY16 pKa = 10.32SSDD19 pKa = 3.65QVEE22 pKa = 4.37TSVTLGSILEE32 pKa = 4.56SIQEE36 pKa = 4.29AIEE39 pKa = 4.34EE40 pKa = 4.28YY41 pKa = 11.26GEE43 pKa = 4.02DD44 pKa = 3.56TKK46 pKa = 11.47VVLYY50 pKa = 10.64NGQRR54 pKa = 11.84YY55 pKa = 7.97GATFGKK61 pKa = 9.93IDD63 pKa = 3.53TEE65 pKa = 4.26YY66 pKa = 10.74GEE68 pKa = 4.76GMISFMDD75 pKa = 3.83PSFAEE80 pKa = 4.24DD81 pKa = 4.38DD82 pKa = 3.92EE83 pKa = 4.81FF84 pKa = 6.57

Molecular weight: 9.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1CJJ3|A0A2P1CJJ3_9CAUD DNA helicase OS=Microbacterium phage Pikmin OX=2099444 GN=37 PE=4 SV=1
MM1 pKa = 7.52SNTRR5 pKa = 11.84VAIPGNVVTTFGRR18 pKa = 11.84IYY20 pKa = 10.89EE21 pKa = 4.32DD22 pKa = 3.28TTKK25 pKa = 10.82EE26 pKa = 3.8ALEE29 pKa = 3.69QHH31 pKa = 5.98LRR33 pKa = 11.84FVISGQAANKK43 pKa = 7.23RR44 pKa = 11.84TNPIYY49 pKa = 10.21KK50 pKa = 9.84RR51 pKa = 11.84EE52 pKa = 4.01VQRR55 pKa = 11.84LKK57 pKa = 10.71RR58 pKa = 11.84AIAARR63 pKa = 3.77

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12813

40

808

217.2

24.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.287 ± 0.462

0.71 ± 0.094

6.689 ± 0.294

6.579 ± 0.334

3.005 ± 0.185

7.734 ± 0.396

1.647 ± 0.182

5.237 ± 0.409

4.839 ± 0.395

8.476 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.149

3.473 ± 0.16

4.925 ± 0.228

3.77 ± 0.226

5.541 ± 0.359

6.415 ± 0.252

6.642 ± 0.374

7.227 ± 0.282

2.162 ± 0.201

3.145 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski