Amino acid dipepetide frequency for Microbacterium phage Pikmin

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.508AlaAla: 8.508 ± 1.078
0.546AlaCys: 0.546 ± 0.219
6.088AlaAsp: 6.088 ± 0.69
5.932AlaGlu: 5.932 ± 0.735
3.122AlaPhe: 3.122 ± 0.474
7.337AlaGly: 7.337 ± 1.281
1.639AlaHis: 1.639 ± 0.345
6.088AlaIle: 6.088 ± 0.966
5.698AlaLys: 5.698 ± 0.923
8.742AlaLeu: 8.742 ± 1.022
2.654AlaMet: 2.654 ± 0.401
2.966AlaAsn: 2.966 ± 0.395
3.434AlaPro: 3.434 ± 0.569
3.044AlaGln: 3.044 ± 0.555
6.244AlaArg: 6.244 ± 0.798
4.761AlaSer: 4.761 ± 0.562
6.556AlaThr: 6.556 ± 0.611
5.464AlaVal: 5.464 ± 0.482
1.561AlaTrp: 1.561 ± 0.288
2.498AlaTyr: 2.498 ± 0.318
0.0AlaXaa: 0.0 ± 0.0
Cys
0.781CysAla: 0.781 ± 0.254
0.078CysCys: 0.078 ± 0.068
0.702CysAsp: 0.702 ± 0.211
0.39CysGlu: 0.39 ± 0.152
0.234CysPhe: 0.234 ± 0.133
0.546CysGly: 0.546 ± 0.25
0.234CysHis: 0.234 ± 0.125
0.078CysIle: 0.078 ± 0.071
0.859CysLys: 0.859 ± 0.272
0.468CysLeu: 0.468 ± 0.18
0.0CysMet: 0.0 ± 0.0
0.078CysAsn: 0.078 ± 0.068
0.468CysPro: 0.468 ± 0.215
0.312CysGln: 0.312 ± 0.146
0.468CysArg: 0.468 ± 0.161
0.234CysSer: 0.234 ± 0.12
0.546CysThr: 0.546 ± 0.216
0.312CysVal: 0.312 ± 0.151
0.156CysTrp: 0.156 ± 0.111
0.156CysTyr: 0.156 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
4.605AspAla: 4.605 ± 0.648
0.546AspCys: 0.546 ± 0.182
6.166AspAsp: 6.166 ± 1.34
5.386AspGlu: 5.386 ± 1.257
2.42AspPhe: 2.42 ± 0.383
4.995AspGly: 4.995 ± 0.593
1.561AspHis: 1.561 ± 0.37
3.668AspIle: 3.668 ± 0.491
2.264AspLys: 2.264 ± 0.33
5.542AspLeu: 5.542 ± 0.754
1.639AspMet: 1.639 ± 0.356
2.107AspAsn: 2.107 ± 0.344
4.059AspPro: 4.059 ± 0.673
2.42AspGln: 2.42 ± 0.351
3.434AspArg: 3.434 ± 0.472
4.059AspSer: 4.059 ± 0.517
3.981AspThr: 3.981 ± 0.736
3.981AspVal: 3.981 ± 0.513
1.561AspTrp: 1.561 ± 0.335
2.498AspTyr: 2.498 ± 0.482
0.0AspXaa: 0.0 ± 0.0
Glu
7.181GluAla: 7.181 ± 0.823
0.39GluCys: 0.39 ± 0.182
5.698GluAsp: 5.698 ± 1.105
5.386GluGlu: 5.386 ± 1.197
1.951GluPhe: 1.951 ± 0.404
5.151GluGly: 5.151 ± 0.519
1.015GluHis: 1.015 ± 0.255
2.576GluIle: 2.576 ± 0.444
2.732GluLys: 2.732 ± 0.508
6.478GluLeu: 6.478 ± 0.635
2.185GluMet: 2.185 ± 0.367
2.498GluAsn: 2.498 ± 0.386
2.029GluPro: 2.029 ± 0.412
1.639GluGln: 1.639 ± 0.334
3.356GluArg: 3.356 ± 0.504
2.888GluSer: 2.888 ± 0.486
4.215GluThr: 4.215 ± 0.529
4.527GluVal: 4.527 ± 0.727
1.873GluTrp: 1.873 ± 0.279
2.029GluTyr: 2.029 ± 0.482
0.0GluXaa: 0.0 ± 0.0
Phe
2.576PheAla: 2.576 ± 0.43
0.312PheCys: 0.312 ± 0.135
1.873PheAsp: 1.873 ± 0.351
1.327PheGlu: 1.327 ± 0.282
0.781PhePhe: 0.781 ± 0.198
2.342PheGly: 2.342 ± 0.448
0.39PheHis: 0.39 ± 0.173
1.327PheIle: 1.327 ± 0.267
1.561PheLys: 1.561 ± 0.306
2.576PheLeu: 2.576 ± 0.452
0.781PheMet: 0.781 ± 0.28
1.405PheAsn: 1.405 ± 0.256
1.561PhePro: 1.561 ± 0.297
1.405PheGln: 1.405 ± 0.326
2.342PheArg: 2.342 ± 0.364
2.42PheSer: 2.42 ± 0.375
2.185PheThr: 2.185 ± 0.464
1.873PheVal: 1.873 ± 0.42
0.39PheTrp: 0.39 ± 0.217
0.624PheTyr: 0.624 ± 0.161
0.0PheXaa: 0.0 ± 0.0
Gly
6.4GlyAla: 6.4 ± 1.027
0.624GlyCys: 0.624 ± 0.221
4.839GlyAsp: 4.839 ± 0.563
4.215GlyGlu: 4.215 ± 0.476
2.185GlyPhe: 2.185 ± 0.335
6.01GlyGly: 6.01 ± 0.975
1.483GlyHis: 1.483 ± 0.34
5.151GlyIle: 5.151 ± 0.844
3.59GlyLys: 3.59 ± 0.526
6.556GlyLeu: 6.556 ± 1.023
2.029GlyMet: 2.029 ± 0.284
2.732GlyAsn: 2.732 ± 0.406
2.029GlyPro: 2.029 ± 0.425
3.356GlyGln: 3.356 ± 0.521
4.527GlyArg: 4.527 ± 0.626
5.854GlySer: 5.854 ± 0.641
5.542GlyThr: 5.542 ± 0.795
6.244GlyVal: 6.244 ± 0.679
1.561GlyTrp: 1.561 ± 0.333
2.42GlyTyr: 2.42 ± 0.432
0.0GlyXaa: 0.0 ± 0.0
His
0.781HisAla: 0.781 ± 0.298
0.234HisCys: 0.234 ± 0.13
1.015HisAsp: 1.015 ± 0.262
1.171HisGlu: 1.171 ± 0.311
0.468HisPhe: 0.468 ± 0.196
1.561HisGly: 1.561 ± 0.378
0.546HisHis: 0.546 ± 0.19
1.639HisIle: 1.639 ± 0.41
0.624HisLys: 0.624 ± 0.246
1.327HisLeu: 1.327 ± 0.35
0.468HisMet: 0.468 ± 0.171
0.468HisAsn: 0.468 ± 0.219
1.015HisPro: 1.015 ± 0.296
0.546HisGln: 0.546 ± 0.244
0.468HisArg: 0.468 ± 0.16
0.937HisSer: 0.937 ± 0.219
0.702HisThr: 0.702 ± 0.209
1.093HisVal: 1.093 ± 0.326
0.702HisTrp: 0.702 ± 0.224
0.702HisTyr: 0.702 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
5.62IleAla: 5.62 ± 0.634
0.39IleCys: 0.39 ± 0.167
3.825IleAsp: 3.825 ± 0.505
3.2IleGlu: 3.2 ± 0.59
0.781IlePhe: 0.781 ± 0.272
3.434IleGly: 3.434 ± 0.844
0.702IleHis: 0.702 ± 0.234
3.59IleIle: 3.59 ± 0.845
2.732IleLys: 2.732 ± 0.435
3.981IleLeu: 3.981 ± 0.531
1.639IleMet: 1.639 ± 0.282
1.717IleAsn: 1.717 ± 0.369
2.732IlePro: 2.732 ± 0.543
2.81IleGln: 2.81 ± 0.647
2.42IleArg: 2.42 ± 0.484
3.356IleSer: 3.356 ± 0.634
3.2IleThr: 3.2 ± 0.578
3.512IleVal: 3.512 ± 0.574
0.702IleTrp: 0.702 ± 0.245
1.951IleTyr: 1.951 ± 0.384
0.0IleXaa: 0.0 ± 0.0
Lys
5.073LysAla: 5.073 ± 0.875
0.312LysCys: 0.312 ± 0.17
2.342LysAsp: 2.342 ± 0.525
3.278LysGlu: 3.278 ± 0.453
1.405LysPhe: 1.405 ± 0.259
3.981LysGly: 3.981 ± 0.497
0.546LysHis: 0.546 ± 0.175
1.717LysIle: 1.717 ± 0.432
3.434LysLys: 3.434 ± 0.768
2.107LysLeu: 2.107 ± 0.499
1.483LysMet: 1.483 ± 0.341
2.107LysAsn: 2.107 ± 0.4
2.966LysPro: 2.966 ± 0.594
1.717LysGln: 1.717 ± 0.291
2.81LysArg: 2.81 ± 0.537
2.888LysSer: 2.888 ± 0.497
4.059LysThr: 4.059 ± 0.682
3.903LysVal: 3.903 ± 0.558
0.781LysTrp: 0.781 ± 0.274
1.483LysTyr: 1.483 ± 0.347
0.0LysXaa: 0.0 ± 0.0
Leu
8.039LeuAla: 8.039 ± 0.938
0.624LeuCys: 0.624 ± 0.211
5.542LeuAsp: 5.542 ± 0.572
5.542LeuGlu: 5.542 ± 0.853
2.42LeuPhe: 2.42 ± 0.357
6.556LeuGly: 6.556 ± 0.827
1.093LeuHis: 1.093 ± 0.264
3.668LeuIle: 3.668 ± 0.802
3.668LeuLys: 3.668 ± 0.583
6.166LeuLeu: 6.166 ± 0.787
1.561LeuMet: 1.561 ± 0.319
2.888LeuAsn: 2.888 ± 0.42
4.995LeuPro: 4.995 ± 0.791
2.654LeuGln: 2.654 ± 0.457
4.839LeuArg: 4.839 ± 0.608
6.01LeuSer: 6.01 ± 0.665
7.493LeuThr: 7.493 ± 0.832
7.337LeuVal: 7.337 ± 0.868
1.483LeuTrp: 1.483 ± 0.251
2.185LeuTyr: 2.185 ± 0.36
0.0LeuXaa: 0.0 ± 0.0
Met
3.59MetAla: 3.59 ± 0.429
0.156MetCys: 0.156 ± 0.099
1.405MetAsp: 1.405 ± 0.311
1.171MetGlu: 1.171 ± 0.321
0.312MetPhe: 0.312 ± 0.13
2.107MetGly: 2.107 ± 0.376
0.312MetHis: 0.312 ± 0.144
0.859MetIle: 0.859 ± 0.219
1.015MetLys: 1.015 ± 0.221
1.717MetLeu: 1.717 ± 0.297
0.781MetMet: 0.781 ± 0.249
0.859MetAsn: 0.859 ± 0.231
1.717MetPro: 1.717 ± 0.337
1.171MetGln: 1.171 ± 0.289
0.937MetArg: 0.937 ± 0.259
2.264MetSer: 2.264 ± 0.435
1.561MetThr: 1.561 ± 0.325
2.264MetVal: 2.264 ± 0.445
0.39MetTrp: 0.39 ± 0.158
0.39MetTyr: 0.39 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
2.888AsnAla: 2.888 ± 0.396
0.234AsnCys: 0.234 ± 0.119
2.185AsnAsp: 2.185 ± 0.405
2.654AsnGlu: 2.654 ± 0.412
0.624AsnPhe: 0.624 ± 0.194
3.356AsnGly: 3.356 ± 0.428
0.546AsnHis: 0.546 ± 0.184
1.483AsnIle: 1.483 ± 0.301
1.717AsnLys: 1.717 ± 0.356
3.59AsnLeu: 3.59 ± 0.512
0.624AsnMet: 0.624 ± 0.196
0.859AsnAsn: 0.859 ± 0.251
2.81AsnPro: 2.81 ± 0.496
1.327AsnGln: 1.327 ± 0.348
1.405AsnArg: 1.405 ± 0.23
2.42AsnSer: 2.42 ± 0.473
1.639AsnThr: 1.639 ± 0.36
2.342AsnVal: 2.342 ± 0.466
0.781AsnTrp: 0.781 ± 0.288
1.249AsnTyr: 1.249 ± 0.356
0.0AsnXaa: 0.0 ± 0.0
Pro
4.917ProAla: 4.917 ± 0.784
0.312ProCys: 0.312 ± 0.172
2.654ProAsp: 2.654 ± 0.534
3.356ProGlu: 3.356 ± 0.539
1.951ProPhe: 1.951 ± 0.388
4.059ProGly: 4.059 ± 0.73
0.859ProHis: 0.859 ± 0.209
1.873ProIle: 1.873 ± 0.306
2.498ProLys: 2.498 ± 0.436
3.746ProLeu: 3.746 ± 0.417
0.624ProMet: 0.624 ± 0.203
2.107ProAsn: 2.107 ± 0.459
2.264ProPro: 2.264 ± 0.347
2.498ProGln: 2.498 ± 0.785
2.576ProArg: 2.576 ± 0.497
3.044ProSer: 3.044 ± 0.399
3.356ProThr: 3.356 ± 0.487
4.449ProVal: 4.449 ± 0.445
0.781ProTrp: 0.781 ± 0.247
1.327ProTyr: 1.327 ± 0.248
0.0ProXaa: 0.0 ± 0.0
Gln
4.839GlnAla: 4.839 ± 0.81
0.156GlnCys: 0.156 ± 0.112
1.873GlnAsp: 1.873 ± 0.361
3.2GlnGlu: 3.2 ± 0.442
0.702GlnPhe: 0.702 ± 0.189
1.873GlnGly: 1.873 ± 0.366
0.781GlnHis: 0.781 ± 0.277
2.81GlnIle: 2.81 ± 0.672
2.029GlnLys: 2.029 ± 0.537
3.512GlnLeu: 3.512 ± 0.814
0.702GlnMet: 0.702 ± 0.235
1.483GlnAsn: 1.483 ± 0.328
0.859GlnPro: 0.859 ± 0.256
1.639GlnGln: 1.639 ± 0.358
2.342GlnArg: 2.342 ± 0.399
2.498GlnSer: 2.498 ± 0.405
2.107GlnThr: 2.107 ± 0.358
2.498GlnVal: 2.498 ± 0.431
0.624GlnTrp: 0.624 ± 0.201
1.171GlnTyr: 1.171 ± 0.238
0.0GlnXaa: 0.0 ± 0.0
Arg
4.137ArgAla: 4.137 ± 0.519
0.234ArgCys: 0.234 ± 0.13
3.2ArgAsp: 3.2 ± 0.492
3.59ArgGlu: 3.59 ± 0.552
2.42ArgPhe: 2.42 ± 0.422
3.434ArgGly: 3.434 ± 0.436
0.702ArgHis: 0.702 ± 0.23
3.044ArgIle: 3.044 ± 0.469
2.81ArgLys: 2.81 ± 0.594
5.932ArgLeu: 5.932 ± 0.882
1.717ArgMet: 1.717 ± 0.32
1.795ArgAsn: 1.795 ± 0.324
2.81ArgPro: 2.81 ± 0.626
1.561ArgGln: 1.561 ± 0.322
3.2ArgArg: 3.2 ± 0.557
3.512ArgSer: 3.512 ± 0.455
3.2ArgThr: 3.2 ± 0.523
4.605ArgVal: 4.605 ± 0.65
1.405ArgTrp: 1.405 ± 0.36
2.107ArgTyr: 2.107 ± 0.411
0.0ArgXaa: 0.0 ± 0.0
Ser
6.166SerAla: 6.166 ± 0.686
0.312SerCys: 0.312 ± 0.136
4.293SerAsp: 4.293 ± 0.54
3.434SerGlu: 3.434 ± 0.644
2.498SerPhe: 2.498 ± 0.526
6.01SerGly: 6.01 ± 0.709
0.781SerHis: 0.781 ± 0.231
2.498SerIle: 2.498 ± 0.434
2.966SerLys: 2.966 ± 0.473
5.698SerLeu: 5.698 ± 0.62
1.561SerMet: 1.561 ± 0.341
1.717SerAsn: 1.717 ± 0.347
3.122SerPro: 3.122 ± 0.47
3.356SerGln: 3.356 ± 0.538
3.903SerArg: 3.903 ± 0.514
4.215SerSer: 4.215 ± 0.52
3.825SerThr: 3.825 ± 0.586
3.981SerVal: 3.981 ± 0.584
1.639SerTrp: 1.639 ± 0.298
2.185SerTyr: 2.185 ± 0.369
0.0SerXaa: 0.0 ± 0.0
Thr
5.073ThrAla: 5.073 ± 0.683
0.234ThrCys: 0.234 ± 0.176
4.605ThrAsp: 4.605 ± 0.657
3.825ThrGlu: 3.825 ± 0.517
1.951ThrPhe: 1.951 ± 0.38
5.464ThrGly: 5.464 ± 0.535
1.171ThrHis: 1.171 ± 0.366
3.2ThrIle: 3.2 ± 0.468
3.278ThrLys: 3.278 ± 0.601
5.62ThrLeu: 5.62 ± 0.755
1.249ThrMet: 1.249 ± 0.319
1.951ThrAsn: 1.951 ± 0.33
4.839ThrPro: 4.839 ± 0.735
1.951ThrGln: 1.951 ± 0.416
4.137ThrArg: 4.137 ± 0.574
4.449ThrSer: 4.449 ± 0.588
3.981ThrThr: 3.981 ± 0.744
5.229ThrVal: 5.229 ± 0.571
1.873ThrTrp: 1.873 ± 0.366
2.342ThrTyr: 2.342 ± 0.561
0.0ThrXaa: 0.0 ± 0.0
Val
7.805ValAla: 7.805 ± 0.75
0.468ValCys: 0.468 ± 0.19
5.308ValAsp: 5.308 ± 0.613
4.371ValGlu: 4.371 ± 0.633
2.185ValPhe: 2.185 ± 0.458
4.917ValGly: 4.917 ± 0.899
0.937ValHis: 0.937 ± 0.281
4.449ValIle: 4.449 ± 0.537
2.654ValLys: 2.654 ± 0.455
6.088ValLeu: 6.088 ± 0.568
1.717ValMet: 1.717 ± 0.322
2.732ValAsn: 2.732 ± 0.483
3.434ValPro: 3.434 ± 0.439
2.966ValGln: 2.966 ± 0.389
3.2ValArg: 3.2 ± 0.386
4.605ValSer: 4.605 ± 0.52
5.386ValThr: 5.386 ± 0.735
5.229ValVal: 5.229 ± 0.74
1.717ValTrp: 1.717 ± 0.316
2.107ValTyr: 2.107 ± 0.402
0.0ValXaa: 0.0 ± 0.0
Trp
1.483TrpAla: 1.483 ± 0.263
0.39TrpCys: 0.39 ± 0.163
1.405TrpAsp: 1.405 ± 0.301
1.405TrpGlu: 1.405 ± 0.272
0.781TrpPhe: 0.781 ± 0.227
1.639TrpGly: 1.639 ± 0.432
0.624TrpHis: 0.624 ± 0.214
1.015TrpIle: 1.015 ± 0.241
0.624TrpLys: 0.624 ± 0.209
2.107TrpLeu: 2.107 ± 0.438
0.39TrpMet: 0.39 ± 0.132
0.937TrpAsn: 0.937 ± 0.26
0.859TrpPro: 0.859 ± 0.278
0.702TrpGln: 0.702 ± 0.287
1.171TrpArg: 1.171 ± 0.281
1.483TrpSer: 1.483 ± 0.434
1.483TrpThr: 1.483 ± 0.379
1.795TrpVal: 1.795 ± 0.363
0.546TrpTrp: 0.546 ± 0.245
0.781TrpTyr: 0.781 ± 0.275
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.42TyrAla: 2.42 ± 0.448
0.546TyrCys: 0.546 ± 0.196
1.873TyrAsp: 1.873 ± 0.377
2.966TyrGlu: 2.966 ± 0.484
0.859TyrPhe: 0.859 ± 0.243
2.576TyrGly: 2.576 ± 0.471
0.546TyrHis: 0.546 ± 0.229
1.639TyrIle: 1.639 ± 0.387
1.639TyrLys: 1.639 ± 0.418
2.81TyrLeu: 2.81 ± 0.429
1.093TyrMet: 1.093 ± 0.264
1.327TyrAsn: 1.327 ± 0.282
1.249TyrPro: 1.249 ± 0.348
0.624TyrGln: 0.624 ± 0.215
1.639TyrArg: 1.639 ± 0.347
2.264TyrSer: 2.264 ± 0.406
1.405TyrThr: 1.405 ± 0.326
1.639TyrVal: 1.639 ± 0.412
1.093TyrTrp: 1.093 ± 0.274
1.249TyrTyr: 1.249 ± 0.331
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (12813 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski