Lichenicoccus roseus
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4254 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5R9J600|A0A5R9J600_9PROT Uncharacterized protein OS=Lichenicoccus roseus OX=2683649 GN=FE263_09950 PE=4 SV=1
MM1 pKa = 7.17 TAFSALSAAAISTQTIATIAALTTADD27 pKa = 4.51 FAPLTAQQMDD37 pKa = 4.33 ALTSAQWAAIVPAALSALTTTQVYY61 pKa = 8.67 TISSTALAGLTSAQLVAMSSTSVEE85 pKa = 4.08 ALEE88 pKa = 5.21 GPQIPGLTIAALAGLSDD105 pKa = 3.89 AEE107 pKa = 3.85 IAGFSEE113 pKa = 3.84 QFMYY117 pKa = 8.99 ITPAQLASLSIDD129 pKa = 3.11 QFQAISSNSLLLLTASQFGAFTTTQIQALTNAQLIGLSGKK169 pKa = 9.31 QCAYY173 pKa = 11.0 LSGPQLAAVSTANLAALTAYY193 pKa = 9.36 QAQSISVAALASLTSTQVAALTPAILASLAGKK225 pKa = 9.85 SLAAISNAALAGATTDD241 pKa = 4.34 EE242 pKa = 4.36 IAGLAGMGQFATLTVAQIAALSPVQLGAITTFEE275 pKa = 4.01 MTGITTAQLQALTAAQIAYY294 pKa = 7.54 LTPAAFATLGSAQLHH309 pKa = 5.96 ALSGAQLNAVSSTDD323 pKa = 3.07 IASISSATIAGLSTAALAGLGSTQIDD349 pKa = 3.96 GLDD352 pKa = 3.01 IAFAGVTTAQLASLTSTTLASLDD375 pKa = 3.41 GRR377 pKa = 11.84 EE378 pKa = 4.15 WASLTTSQIASFTTAQIASLAPAALAAIPTANYY411 pKa = 10.57 AGLTYY416 pKa = 10.95 ADD418 pKa = 3.43 IAALTTAQVAALVPSEE434 pKa = 3.94 ILSFSEE440 pKa = 3.98 LQIASLTKK448 pKa = 9.64 PQVASISITDD458 pKa = 3.8 IAALGTDD465 pKa = 4.17 PARR468 pKa = 11.84 MLSTNAIAGLTATQIVAMASTSVEE492 pKa = 3.98 ALTEE496 pKa = 4.09 NQVPALSLATFDD508 pKa = 4.39 ALTPAEE514 pKa = 3.97 LAGFVNNQIAFMTVAEE530 pKa = 4.37 VASMSVLQIQALGSNLLTQLTAPQLAALTTAQIAALTTTQVAGLGSTQFAAFSAPQAAALNLADD594 pKa = 5.44 LAGLTTNQATSLSLAALAALTSTQVAAISSSALEE628 pKa = 4.02 ALSARR633 pKa = 11.84 GIAAITTAALAGVSTDD649 pKa = 3.87 EE650 pKa = 4.23 IAALTAYY657 pKa = 10.52 ASGQFQALTAAQIAALSTAQVAAITSGEE685 pKa = 4.09 VLGITSAQLQALTPRR700 pKa = 11.84 EE701 pKa = 3.91 IAALTSVQAGTMTSTQLL718 pKa = 3.3
Molecular weight: 70.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.452
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.554
Rodwell 3.478
Grimsley 3.363
Solomon 3.617
Lehninger 3.567
Nozaki 3.757
DTASelect 3.935
Thurlkill 3.503
EMBOSS 3.567
Sillero 3.77
Patrickios 0.947
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.707
Protein with the highest isoelectric point:
>tr|A0A5R9J9H8|A0A5R9J9H8_9PROT SDR family oxidoreductase OS=Lichenicoccus roseus OX=2683649 GN=FE263_03185 PE=4 SV=1
MM1 pKa = 7.39 RR2 pKa = 11.84 TLLTLLMVFAMIGTVGVLAAGVVGFVRR29 pKa = 11.84 GGGDD33 pKa = 3.16 PRR35 pKa = 11.84 RR36 pKa = 11.84 SNRR39 pKa = 11.84 LMRR42 pKa = 11.84 WRR44 pKa = 11.84 VILQASAILLFVLLLTIFRR63 pKa = 11.84 RR64 pKa = 3.89
Molecular weight: 7.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.774
IPC_protein 12.384
Toseland 12.544
ProMoST 13.056
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.047
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.798
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4254
0
4254
1424553
26
8351
334.9
35.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.381 ± 0.051
0.925 ± 0.012
5.543 ± 0.029
4.793 ± 0.044
3.214 ± 0.025
9.18 ± 0.042
2.315 ± 0.019
4.606 ± 0.029
1.877 ± 0.028
11.037 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.31 ± 0.02
2.186 ± 0.023
5.707 ± 0.032
3.292 ± 0.028
7.898 ± 0.063
5.528 ± 0.048
5.373 ± 0.083
7.478 ± 0.03
1.378 ± 0.015
1.978 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here