Inquilinus limosus
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7046 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A211Z046|A0A211Z046_9PROT Aspartate-semialdehyde dehydrogenase OS=Inquilinus limosus OX=171674 GN=asd PE=3 SV=1
MM1 pKa = 7.66 PLISNIAYY9 pKa = 10.47 SPDD12 pKa = 3.61 PTSGQALGLDD22 pKa = 3.73 VALNGATRR30 pKa = 11.84 LVVNWGDD37 pKa = 3.66 GSPLQLYY44 pKa = 8.18 QVEE47 pKa = 4.63 PGSSSIHH54 pKa = 6.51 LSHH57 pKa = 7.31 AYY59 pKa = 10.17 ADD61 pKa = 3.9 PTVGTVVLRR70 pKa = 11.84 AGGPGVPDD78 pKa = 4.65 DD79 pKa = 4.1 RR80 pKa = 11.84 ATLNLVVGADD90 pKa = 3.55 EE91 pKa = 4.56 SYY93 pKa = 11.39 SRR95 pKa = 11.84 VSGDD99 pKa = 3.58 PEE101 pKa = 4.18 FDD103 pKa = 3.48 TILRR107 pKa = 11.84 EE108 pKa = 3.99 NPLISGDD115 pKa = 3.72 GNLVAWADD123 pKa = 4.16 TINGHH128 pKa = 5.89 SVFAMVKK135 pKa = 9.68 NLATGTTDD143 pKa = 3.23 YY144 pKa = 11.35 LGVGRR149 pKa = 11.84 DD150 pKa = 3.5 AYY152 pKa = 11.05 QFDD155 pKa = 4.69 GPPVMLANGAAVWVYY170 pKa = 10.77 EE171 pKa = 4.64 GYY173 pKa = 10.8 LGIAGYY179 pKa = 8.58 LHH181 pKa = 7.02 PASLYY186 pKa = 10.55 LAATGQEE193 pKa = 3.78 ITLEE197 pKa = 4.16 DD198 pKa = 4.28 AYY200 pKa = 10.74 HH201 pKa = 5.8 ITVTDD206 pKa = 3.76 DD207 pKa = 2.9 AKK209 pKa = 10.92 YY210 pKa = 10.65 AVYY213 pKa = 10.19 QYY215 pKa = 10.01 DD216 pKa = 3.79 TEE218 pKa = 4.78 LGSGQNGWYY227 pKa = 10.49 LRR229 pKa = 11.84 DD230 pKa = 3.54 LDD232 pKa = 4.97 AGTSVYY238 pKa = 10.69 LGADD242 pKa = 3.51 SEE244 pKa = 4.82 YY245 pKa = 10.9 DD246 pKa = 3.21 GPRR249 pKa = 11.84 YY250 pKa = 9.21 HH251 pKa = 6.64 VHH253 pKa = 6.61 FVDD256 pKa = 4.12 GQLVVDD262 pKa = 5.2 DD263 pKa = 5.76 DD264 pKa = 4.34 STGQQSVSGALATDD278 pKa = 4.12 TTADD282 pKa = 3.31 GSVTVIATDD291 pKa = 3.65 DD292 pKa = 3.61 ALAPGDD298 pKa = 4.11 TNNGSDD304 pKa = 4.62 VYY306 pKa = 10.6 LQEE309 pKa = 4.63 NGSPTHH315 pKa = 7.29 DD316 pKa = 3.31 GTAGPDD322 pKa = 3.54 VLLLGAANDD331 pKa = 3.87 IADD334 pKa = 3.96 GGAGKK339 pKa = 10.35 DD340 pKa = 3.82 YY341 pKa = 10.97 LAGGAGDD348 pKa = 4.58 DD349 pKa = 4.32 SLSGGAGDD357 pKa = 5.54 DD358 pKa = 3.99 LLEE361 pKa = 4.9 GGAGADD367 pKa = 3.9 SLNGGDD373 pKa = 4.88 GIDD376 pKa = 3.41 TAGYY380 pKa = 7.28 ATSLAGVQASLDD392 pKa = 3.66 RR393 pKa = 11.84 PAGNTGAAAGDD404 pKa = 3.95 SYY406 pKa = 12.06 AGIEE410 pKa = 4.15 NLLGSAFADD419 pKa = 3.64 RR420 pKa = 11.84 LEE422 pKa = 5.19 GDD424 pKa = 3.34 AGANALRR431 pKa = 11.84 GGAGDD436 pKa = 4.41 DD437 pKa = 3.42 RR438 pKa = 11.84 LGGLGGDD445 pKa = 3.98 DD446 pKa = 3.64 RR447 pKa = 11.84 LVGGDD452 pKa = 3.61 GGDD455 pKa = 3.65 IISGGSGNDD464 pKa = 3.33 RR465 pKa = 11.84 IYY467 pKa = 11.29 GGAGDD472 pKa = 5.03 DD473 pKa = 4.25 RR474 pKa = 11.84 MTGGAGADD482 pKa = 3.27 VFVIQPGFGRR492 pKa = 11.84 DD493 pKa = 3.24 VVTDD497 pKa = 4.53 FSHH500 pKa = 7.39 AAGDD504 pKa = 3.85 VILFDD509 pKa = 4.21 RR510 pKa = 11.84 DD511 pKa = 3.35 VFASFAEE518 pKa = 4.67 VMAHH522 pKa = 6.13 AADD525 pKa = 3.92 YY526 pKa = 10.57 GADD529 pKa = 3.61 GTVIALDD536 pKa = 4.3 ADD538 pKa = 4.07 DD539 pKa = 5.69 KK540 pKa = 11.67 LILLGVDD547 pKa = 3.15 RR548 pKa = 11.84 ASLVAGDD555 pKa = 3.78 FAFVV559 pKa = 3.08
Molecular weight: 56.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.666
IPC2_protein 3.668
IPC_protein 3.732
Toseland 3.49
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.389
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.19
Thurlkill 3.554
EMBOSS 3.732
Sillero 3.859
Patrickios 1.456
IPC_peptide 3.745
IPC2_peptide 3.834
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|A0A211ZIN8|A0A211ZIN8_9PROT MFS transporter OS=Inquilinus limosus OX=171674 GN=BWR60_20960 PE=4 SV=1
MM1 pKa = 7.2 PRR3 pKa = 11.84 EE4 pKa = 3.92 RR5 pKa = 11.84 QRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 ATVCVVSVSSQAMTSASRR27 pKa = 11.84 TRR29 pKa = 11.84 AASPARR35 pKa = 11.84 IPGIRR40 pKa = 11.84 AGSRR44 pKa = 11.84 RR45 pKa = 11.84 SAKK48 pKa = 9.97 LRR50 pKa = 11.84 STAATAASRR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 ARR63 pKa = 11.84 SASTLAASPAGRR75 pKa = 11.84 AGSGVPGQAGDD86 pKa = 3.79 QRR88 pKa = 11.84 PHH90 pKa = 7.18 SSASPRR96 pKa = 11.84 PQARR100 pKa = 11.84 PAITEE105 pKa = 3.91 PNGRR109 pKa = 11.84 SSTRR113 pKa = 11.84 RR114 pKa = 11.84 VSSSS118 pKa = 2.59
Molecular weight: 12.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.364
IPC2_protein 10.789
IPC_protein 12.398
Toseland 12.559
ProMoST 13.056
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.076
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 11.798
IPC_peptide 13.056
IPC2_peptide 12.047
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7046
0
7046
2248913
26
3549
319.2
34.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.76 ± 0.043
0.773 ± 0.009
5.881 ± 0.029
5.126 ± 0.026
3.463 ± 0.018
9.411 ± 0.041
1.943 ± 0.014
4.729 ± 0.021
2.339 ± 0.023
10.662 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.141 ± 0.013
2.067 ± 0.018
5.791 ± 0.023
3.044 ± 0.016
7.755 ± 0.037
4.769 ± 0.021
5.181 ± 0.022
7.601 ± 0.021
1.456 ± 0.013
2.103 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here