Acinetobacter phage Presley
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5PZZ5|U5PZZ5_9CAUD Structural protein OS=Acinetobacter phage Presley OX=1406780 GN=Presley_81 PE=4 SV=1
MM1 pKa = 7.46 INVIEE6 pKa = 4.39 QYY8 pKa = 9.67 TVSDD12 pKa = 3.61 QCVMQLNHH20 pKa = 6.93 HH21 pKa = 6.63 IEE23 pKa = 4.11 AGIYY27 pKa = 9.11 TVDD30 pKa = 3.53 LNYY33 pKa = 10.42 MNGAVIATMMGSKK46 pKa = 10.35 NEE48 pKa = 4.14 STATWFYY55 pKa = 11.24 EE56 pKa = 3.92 NLEE59 pKa = 4.05 GSYY62 pKa = 11.12 EE63 pKa = 3.98 EE64 pKa = 4.25 LAEE67 pKa = 4.13 VQANGEE73 pKa = 4.12
Molecular weight: 8.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.011
IPC2_protein 4.088
IPC_protein 3.884
Toseland 3.757
ProMoST 3.935
Dawson 3.821
Bjellqvist 4.088
Wikipedia 3.694
Rodwell 3.732
Grimsley 3.681
Solomon 3.795
Lehninger 3.745
Nozaki 3.973
DTASelect 3.999
Thurlkill 3.783
EMBOSS 3.719
Sillero 3.999
Patrickios 0.006
IPC_peptide 3.808
IPC2_peptide 3.973
IPC2.peptide.svr19 3.99
Protein with the highest isoelectric point:
>tr|U5PVX8|U5PVX8_9CAUD Uncharacterized protein OS=Acinetobacter phage Presley OX=1406780 GN=Presley_78 PE=4 SV=1
MM1 pKa = 7.4 NKK3 pKa = 10.31 AIMVVGVVIGSIILITVVAYY23 pKa = 9.74 VISLIVMVVTYY34 pKa = 8.53 VLPFVVIVFCIGYY47 pKa = 10.31 ASCLYY52 pKa = 10.49 NKK54 pKa = 9.95 RR55 pKa = 11.84 KK56 pKa = 9.99 KK57 pKa = 10.0 RR58 pKa = 11.84 DD59 pKa = 3.17 RR60 pKa = 3.84
Molecular weight: 6.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.197
IPC2_protein 9.487
IPC_protein 9.56
Toseland 9.78
ProMoST 9.823
Dawson 10.072
Bjellqvist 9.838
Wikipedia 10.277
Rodwell 10.335
Grimsley 10.16
Solomon 10.131
Lehninger 10.087
Nozaki 9.882
DTASelect 9.794
Thurlkill 9.897
EMBOSS 10.204
Sillero 9.999
Patrickios 7.395
IPC_peptide 10.116
IPC2_peptide 8.858
IPC2.peptide.svr19 8.304
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
94
0
94
24559
20
3492
261.3
29.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.928 ± 0.447
0.745 ± 0.132
6.564 ± 0.197
6.515 ± 0.249
3.665 ± 0.205
5.937 ± 0.245
2.02 ± 0.171
6.735 ± 0.301
6.657 ± 0.218
8.608 ± 0.215
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.765 ± 0.157
5.904 ± 0.253
3.766 ± 0.165
4.52 ± 0.291
4.247 ± 0.135
5.859 ± 0.251
6.368 ± 0.257
6.128 ± 0.221
1.022 ± 0.102
4.047 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here