Amino acid dipepetide frequency for Acinetobacter phage Presley

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.474AlaAla: 6.474 ± 0.71
0.692AlaCys: 0.692 ± 0.164
4.601AlaAsp: 4.601 ± 0.492
5.66AlaGlu: 5.66 ± 0.552
2.565AlaPhe: 2.565 ± 0.353
4.153AlaGly: 4.153 ± 0.483
1.099AlaHis: 1.099 ± 0.163
5.579AlaIle: 5.579 ± 0.638
5.986AlaLys: 5.986 ± 0.565
7.818AlaLeu: 7.818 ± 0.73
2.565AlaMet: 2.565 ± 0.309
4.764AlaAsn: 4.764 ± 0.541
2.117AlaPro: 2.117 ± 0.327
3.95AlaGln: 3.95 ± 0.699
3.624AlaArg: 3.624 ± 0.364
4.316AlaSer: 4.316 ± 0.551
5.375AlaThr: 5.375 ± 0.638
4.52AlaVal: 4.52 ± 0.467
0.774AlaTrp: 0.774 ± 0.147
2.647AlaTyr: 2.647 ± 0.302
0.0AlaXaa: 0.0 ± 0.0
Cys
0.652CysAla: 0.652 ± 0.164
0.041CysCys: 0.041 ± 0.046
0.326CysAsp: 0.326 ± 0.126
0.244CysGlu: 0.244 ± 0.092
0.244CysPhe: 0.244 ± 0.092
0.366CysGly: 0.366 ± 0.148
0.326CysHis: 0.326 ± 0.121
0.896CysIle: 0.896 ± 0.247
0.407CysLys: 0.407 ± 0.179
0.57CysLeu: 0.57 ± 0.194
0.204CysMet: 0.204 ± 0.098
0.407CysAsn: 0.407 ± 0.117
0.163CysPro: 0.163 ± 0.082
0.163CysGln: 0.163 ± 0.081
0.489CysArg: 0.489 ± 0.153
0.733CysSer: 0.733 ± 0.244
0.407CysThr: 0.407 ± 0.157
0.366CysVal: 0.366 ± 0.129
0.163CysTrp: 0.163 ± 0.078
0.285CysTyr: 0.285 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
4.846AspAla: 4.846 ± 0.53
0.489AspCys: 0.489 ± 0.171
4.153AspAsp: 4.153 ± 0.637
4.031AspGlu: 4.031 ± 0.485
3.013AspPhe: 3.013 ± 0.378
3.624AspGly: 3.624 ± 0.568
1.507AspHis: 1.507 ± 0.234
5.375AspIle: 5.375 ± 0.409
4.357AspLys: 4.357 ± 0.482
5.294AspLeu: 5.294 ± 0.397
1.384AspMet: 1.384 ± 0.214
3.543AspAsn: 3.543 ± 0.413
3.706AspPro: 3.706 ± 0.358
2.484AspGln: 2.484 ± 0.445
2.606AspArg: 2.606 ± 0.3
3.135AspSer: 3.135 ± 0.349
3.95AspThr: 3.95 ± 0.402
4.235AspVal: 4.235 ± 0.486
1.099AspTrp: 1.099 ± 0.212
2.81AspTyr: 2.81 ± 0.447
0.0AspXaa: 0.0 ± 0.0
Glu
5.375GluAla: 5.375 ± 0.68
0.489GluCys: 0.489 ± 0.157
3.991GluAsp: 3.991 ± 0.537
4.927GluGlu: 4.927 ± 0.669
2.647GluPhe: 2.647 ± 0.351
3.502GluGly: 3.502 ± 0.369
1.466GluHis: 1.466 ± 0.256
4.642GluIle: 4.642 ± 0.524
3.176GluLys: 3.176 ± 0.31
7.004GluLeu: 7.004 ± 0.706
1.751GluMet: 1.751 ± 0.247
3.217GluAsn: 3.217 ± 0.429
1.751GluPro: 1.751 ± 0.315
3.991GluGln: 3.991 ± 0.451
2.81GluArg: 2.81 ± 0.32
3.706GluSer: 3.706 ± 0.458
3.054GluThr: 3.054 ± 0.391
4.601GluVal: 4.601 ± 0.406
0.652GluTrp: 0.652 ± 0.17
2.362GluTyr: 2.362 ± 0.385
0.0GluXaa: 0.0 ± 0.0
Phe
1.955PheAla: 1.955 ± 0.247
0.285PheCys: 0.285 ± 0.103
2.973PheAsp: 2.973 ± 0.348
1.873PheGlu: 1.873 ± 0.326
1.222PhePhe: 1.222 ± 0.286
2.443PheGly: 2.443 ± 0.34
0.855PheHis: 0.855 ± 0.199
2.688PheIle: 2.688 ± 0.359
2.769PheLys: 2.769 ± 0.365
2.769PheLeu: 2.769 ± 0.434
1.099PheMet: 1.099 ± 0.19
2.769PheAsn: 2.769 ± 0.374
1.588PhePro: 1.588 ± 0.291
1.018PheGln: 1.018 ± 0.194
1.873PheArg: 1.873 ± 0.288
2.077PheSer: 2.077 ± 0.259
2.688PheThr: 2.688 ± 0.277
1.995PheVal: 1.995 ± 0.367
0.244PheTrp: 0.244 ± 0.119
1.466PheTyr: 1.466 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
3.135GlyAla: 3.135 ± 0.33
0.244GlyCys: 0.244 ± 0.122
2.932GlyAsp: 2.932 ± 0.427
4.194GlyGlu: 4.194 ± 0.401
2.891GlyPhe: 2.891 ± 0.39
3.38GlyGly: 3.38 ± 0.477
1.303GlyHis: 1.303 ± 0.269
3.868GlyIle: 3.868 ± 0.386
5.131GlyLys: 5.131 ± 0.512
4.764GlyLeu: 4.764 ± 0.437
1.71GlyMet: 1.71 ± 0.307
3.624GlyAsn: 3.624 ± 0.394
1.262GlyPro: 1.262 ± 0.266
2.443GlyGln: 2.443 ± 0.283
2.81GlyArg: 2.81 ± 0.434
4.031GlySer: 4.031 ± 0.445
4.153GlyThr: 4.153 ± 0.597
3.787GlyVal: 3.787 ± 0.558
0.652GlyTrp: 0.652 ± 0.194
3.054GlyTyr: 3.054 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
1.384HisAla: 1.384 ± 0.236
0.204HisCys: 0.204 ± 0.091
1.507HisAsp: 1.507 ± 0.327
0.977HisGlu: 0.977 ± 0.242
0.896HisPhe: 0.896 ± 0.207
1.14HisGly: 1.14 ± 0.2
0.204HisHis: 0.204 ± 0.133
1.67HisIle: 1.67 ± 0.282
1.344HisLys: 1.344 ± 0.313
1.914HisLeu: 1.914 ± 0.322
0.611HisMet: 0.611 ± 0.131
0.774HisAsn: 0.774 ± 0.185
0.937HisPro: 0.937 ± 0.179
0.855HisGln: 0.855 ± 0.185
0.937HisArg: 0.937 ± 0.182
1.181HisSer: 1.181 ± 0.242
1.181HisThr: 1.181 ± 0.211
1.507HisVal: 1.507 ± 0.274
0.244HisTrp: 0.244 ± 0.101
0.733HisTyr: 0.733 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
5.334IleAla: 5.334 ± 0.541
0.57IleCys: 0.57 ± 0.185
5.619IleAsp: 5.619 ± 0.521
4.52IleGlu: 4.52 ± 0.522
1.792IlePhe: 1.792 ± 0.302
3.787IleGly: 3.787 ± 0.381
1.507IleHis: 1.507 ± 0.343
3.787IleIle: 3.787 ± 0.528
4.601IleLys: 4.601 ± 0.464
4.968IleLeu: 4.968 ± 0.461
1.792IleMet: 1.792 ± 0.297
4.764IleAsn: 4.764 ± 0.528
3.258IlePro: 3.258 ± 0.454
3.42IleGln: 3.42 ± 0.435
2.606IleArg: 2.606 ± 0.255
3.909IleSer: 3.909 ± 0.341
4.927IleThr: 4.927 ± 0.664
3.135IleVal: 3.135 ± 0.362
0.57IleTrp: 0.57 ± 0.166
2.484IleTyr: 2.484 ± 0.455
0.0IleXaa: 0.0 ± 0.0
Lys
7.37LysAla: 7.37 ± 0.736
0.448LysCys: 0.448 ± 0.162
4.805LysAsp: 4.805 ± 0.48
4.357LysGlu: 4.357 ± 0.564
1.995LysPhe: 1.995 ± 0.32
3.787LysGly: 3.787 ± 0.423
1.792LysHis: 1.792 ± 0.342
3.42LysIle: 3.42 ± 0.412
3.868LysLys: 3.868 ± 0.516
6.434LysLeu: 6.434 ± 0.545
1.914LysMet: 1.914 ± 0.362
3.013LysAsn: 3.013 ± 0.338
2.199LysPro: 2.199 ± 0.388
2.891LysGln: 2.891 ± 0.417
3.135LysArg: 3.135 ± 0.443
3.054LysSer: 3.054 ± 0.297
4.357LysThr: 4.357 ± 0.419
4.072LysVal: 4.072 ± 0.489
0.57LysTrp: 0.57 ± 0.154
3.095LysTyr: 3.095 ± 0.389
0.0LysXaa: 0.0 ± 0.0
Leu
6.312LeuAla: 6.312 ± 0.596
0.855LeuCys: 0.855 ± 0.242
6.841LeuAsp: 6.841 ± 0.398
5.823LeuGlu: 5.823 ± 0.56
2.85LeuPhe: 2.85 ± 0.436
5.864LeuGly: 5.864 ± 0.534
1.588LeuHis: 1.588 ± 0.285
5.619LeuIle: 5.619 ± 0.626
5.049LeuLys: 5.049 ± 0.483
6.434LeuLeu: 6.434 ± 0.491
2.85LeuMet: 2.85 ± 0.409
5.538LeuAsn: 5.538 ± 0.537
3.502LeuPro: 3.502 ± 0.406
3.217LeuGln: 3.217 ± 0.355
3.583LeuArg: 3.583 ± 0.398
5.66LeuSer: 5.66 ± 0.587
6.189LeuThr: 6.189 ± 0.571
4.642LeuVal: 4.642 ± 0.419
0.733LeuTrp: 0.733 ± 0.181
2.932LeuTyr: 2.932 ± 0.313
0.0LeuXaa: 0.0 ± 0.0
Met
2.688MetAla: 2.688 ± 0.343
0.122MetCys: 0.122 ± 0.077
1.955MetAsp: 1.955 ± 0.293
1.303MetGlu: 1.303 ± 0.231
1.222MetPhe: 1.222 ± 0.182
1.588MetGly: 1.588 ± 0.258
0.448MetHis: 0.448 ± 0.16
1.588MetIle: 1.588 ± 0.315
1.832MetLys: 1.832 ± 0.357
1.832MetLeu: 1.832 ± 0.207
0.611MetMet: 0.611 ± 0.193
1.629MetAsn: 1.629 ± 0.372
1.344MetPro: 1.344 ± 0.281
1.059MetGln: 1.059 ± 0.194
1.425MetArg: 1.425 ± 0.204
2.117MetSer: 2.117 ± 0.351
1.629MetThr: 1.629 ± 0.254
1.914MetVal: 1.914 ± 0.298
0.285MetTrp: 0.285 ± 0.109
1.059MetTyr: 1.059 ± 0.23
0.0MetXaa: 0.0 ± 0.0
Asn
5.375AsnAla: 5.375 ± 0.621
0.204AsnCys: 0.204 ± 0.098
3.176AsnAsp: 3.176 ± 0.449
3.665AsnGlu: 3.665 ± 0.3
2.117AsnPhe: 2.117 ± 0.278
3.339AsnGly: 3.339 ± 0.66
1.425AsnHis: 1.425 ± 0.221
3.665AsnIle: 3.665 ± 0.521
3.787AsnLys: 3.787 ± 0.407
5.212AsnLeu: 5.212 ± 0.538
1.507AsnMet: 1.507 ± 0.292
3.054AsnAsn: 3.054 ± 0.628
3.339AsnPro: 3.339 ± 0.468
3.339AsnGln: 3.339 ± 0.35
2.647AsnArg: 2.647 ± 0.272
3.909AsnSer: 3.909 ± 0.499
3.176AsnThr: 3.176 ± 0.523
3.217AsnVal: 3.217 ± 0.387
0.692AsnTrp: 0.692 ± 0.2
2.199AsnTyr: 2.199 ± 0.332
0.0AsnXaa: 0.0 ± 0.0
Pro
2.402ProAla: 2.402 ± 0.261
0.326ProCys: 0.326 ± 0.12
2.932ProAsp: 2.932 ± 0.346
3.054ProGlu: 3.054 ± 0.434
1.914ProPhe: 1.914 ± 0.22
1.507ProGly: 1.507 ± 0.235
0.937ProHis: 0.937 ± 0.174
2.565ProIle: 2.565 ± 0.344
2.525ProLys: 2.525 ± 0.392
3.013ProLeu: 3.013 ± 0.438
0.937ProMet: 0.937 ± 0.22
3.054ProAsn: 3.054 ± 0.353
0.855ProPro: 0.855 ± 0.205
1.344ProGln: 1.344 ± 0.252
1.059ProArg: 1.059 ± 0.208
2.28ProSer: 2.28 ± 0.337
2.362ProThr: 2.362 ± 0.375
2.85ProVal: 2.85 ± 0.431
0.326ProTrp: 0.326 ± 0.14
1.425ProTyr: 1.425 ± 0.223
0.0ProXaa: 0.0 ± 0.0
Gln
3.624GlnAla: 3.624 ± 0.518
0.326GlnCys: 0.326 ± 0.146
2.28GlnAsp: 2.28 ± 0.336
3.502GlnGlu: 3.502 ± 0.419
1.303GlnPhe: 1.303 ± 0.174
2.81GlnGly: 2.81 ± 0.319
0.448GlnHis: 0.448 ± 0.166
3.176GlnIle: 3.176 ± 0.466
3.013GlnLys: 3.013 ± 0.421
4.398GlnLeu: 4.398 ± 0.485
1.018GlnMet: 1.018 ± 0.208
1.955GlnAsn: 1.955 ± 0.352
1.262GlnPro: 1.262 ± 0.244
3.258GlnGln: 3.258 ± 0.658
2.117GlnArg: 2.117 ± 0.398
2.81GlnSer: 2.81 ± 0.43
2.565GlnThr: 2.565 ± 0.431
2.484GlnVal: 2.484 ± 0.423
0.489GlnTrp: 0.489 ± 0.119
2.362GlnTyr: 2.362 ± 0.316
0.0GlnXaa: 0.0 ± 0.0
Arg
2.891ArgAla: 2.891 ± 0.416
0.326ArgCys: 0.326 ± 0.132
2.565ArgAsp: 2.565 ± 0.401
2.484ArgGlu: 2.484 ± 0.325
1.262ArgPhe: 1.262 ± 0.241
2.81ArgGly: 2.81 ± 0.368
0.855ArgHis: 0.855 ± 0.196
3.502ArgIle: 3.502 ± 0.333
3.135ArgLys: 3.135 ± 0.346
3.543ArgLeu: 3.543 ± 0.421
1.018ArgMet: 1.018 ± 0.224
2.81ArgAsn: 2.81 ± 0.356
1.099ArgPro: 1.099 ± 0.24
1.832ArgGln: 1.832 ± 0.246
1.099ArgArg: 1.099 ± 0.196
2.688ArgSer: 2.688 ± 0.33
2.973ArgThr: 2.973 ± 0.423
2.85ArgVal: 2.85 ± 0.488
0.529ArgTrp: 0.529 ± 0.19
2.199ArgTyr: 2.199 ± 0.327
0.0ArgXaa: 0.0 ± 0.0
Ser
4.357SerAla: 4.357 ± 0.63
0.366SerCys: 0.366 ± 0.147
3.461SerAsp: 3.461 ± 0.378
3.339SerGlu: 3.339 ± 0.371
1.995SerPhe: 1.995 ± 0.292
4.479SerGly: 4.479 ± 0.419
0.977SerHis: 0.977 ± 0.223
4.113SerIle: 4.113 ± 0.509
4.235SerLys: 4.235 ± 0.473
5.375SerLeu: 5.375 ± 0.528
1.71SerMet: 1.71 ± 0.25
3.665SerAsn: 3.665 ± 0.412
2.199SerPro: 2.199 ± 0.502
2.525SerGln: 2.525 ± 0.308
2.525SerArg: 2.525 ± 0.277
3.217SerSer: 3.217 ± 0.369
3.42SerThr: 3.42 ± 0.342
3.583SerVal: 3.583 ± 0.322
0.652SerTrp: 0.652 ± 0.18
2.402SerTyr: 2.402 ± 0.268
0.0SerXaa: 0.0 ± 0.0
Thr
6.434ThrAla: 6.434 ± 0.748
0.448ThrCys: 0.448 ± 0.144
4.52ThrAsp: 4.52 ± 0.417
3.746ThrGlu: 3.746 ± 0.587
2.321ThrPhe: 2.321 ± 0.382
3.665ThrGly: 3.665 ± 0.412
1.303ThrHis: 1.303 ± 0.223
3.868ThrIle: 3.868 ± 0.59
4.276ThrLys: 4.276 ± 0.44
4.846ThrLeu: 4.846 ± 0.457
1.67ThrMet: 1.67 ± 0.25
3.991ThrAsn: 3.991 ± 0.591
2.525ThrPro: 2.525 ± 0.318
2.606ThrGln: 2.606 ± 0.339
2.769ThrArg: 2.769 ± 0.305
3.461ThrSer: 3.461 ± 0.421
3.991ThrThr: 3.991 ± 0.38
4.235ThrVal: 4.235 ± 0.56
0.611ThrTrp: 0.611 ± 0.179
2.402ThrTyr: 2.402 ± 0.341
0.0ThrXaa: 0.0 ± 0.0
Val
4.398ValAla: 4.398 ± 0.535
0.326ValCys: 0.326 ± 0.136
3.991ValAsp: 3.991 ± 0.37
3.991ValGlu: 3.991 ± 0.438
2.24ValPhe: 2.24 ± 0.407
3.583ValGly: 3.583 ± 0.521
1.425ValHis: 1.425 ± 0.244
3.909ValIle: 3.909 ± 0.514
3.95ValLys: 3.95 ± 0.366
5.09ValLeu: 5.09 ± 0.491
2.117ValMet: 2.117 ± 0.273
3.828ValAsn: 3.828 ± 0.494
2.688ValPro: 2.688 ± 0.328
2.117ValGln: 2.117 ± 0.297
2.606ValArg: 2.606 ± 0.3
3.991ValSer: 3.991 ± 0.365
4.276ValThr: 4.276 ± 0.56
3.706ValVal: 3.706 ± 0.443
0.448ValTrp: 0.448 ± 0.126
2.606ValTyr: 2.606 ± 0.447
0.0ValXaa: 0.0 ± 0.0
Trp
0.814TrpAla: 0.814 ± 0.182
0.163TrpCys: 0.163 ± 0.101
0.529TrpAsp: 0.529 ± 0.133
0.57TrpGlu: 0.57 ± 0.199
0.366TrpPhe: 0.366 ± 0.126
0.896TrpGly: 0.896 ± 0.215
0.204TrpHis: 0.204 ± 0.096
0.774TrpIle: 0.774 ± 0.208
0.489TrpLys: 0.489 ± 0.15
0.855TrpLeu: 0.855 ± 0.229
0.204TrpMet: 0.204 ± 0.082
0.529TrpAsn: 0.529 ± 0.123
0.407TrpPro: 0.407 ± 0.176
0.611TrpGln: 0.611 ± 0.181
0.285TrpArg: 0.285 ± 0.13
0.57TrpSer: 0.57 ± 0.168
0.733TrpThr: 0.733 ± 0.196
0.733TrpVal: 0.733 ± 0.172
0.041TrpTrp: 0.041 ± 0.049
0.448TrpTyr: 0.448 ± 0.101
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.461TyrAla: 3.461 ± 0.446
0.529TyrCys: 0.529 ± 0.173
2.484TyrAsp: 2.484 ± 0.307
2.891TyrGlu: 2.891 ± 0.328
1.792TyrPhe: 1.792 ± 0.279
2.647TyrGly: 2.647 ± 0.387
0.529TyrHis: 0.529 ± 0.156
2.647TyrIle: 2.647 ± 0.329
2.647TyrLys: 2.647 ± 0.318
3.95TyrLeu: 3.95 ± 0.44
0.937TyrMet: 0.937 ± 0.228
2.117TyrAsn: 2.117 ± 0.293
1.466TyrPro: 1.466 ± 0.336
2.077TyrGln: 2.077 ± 0.298
1.466TyrArg: 1.466 ± 0.244
1.751TyrSer: 1.751 ± 0.246
2.28TyrThr: 2.28 ± 0.339
2.85TyrVal: 2.85 ± 0.369
0.448TyrTrp: 0.448 ± 0.163
1.507TyrTyr: 1.507 ± 0.284
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (24559 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski