Frankia alni (strain ACN14a)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia; Frankia alni

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6710 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0RRD1|Q0RRD1_FRAAA Glucose-1-phosphate thymidylyltransferase (DTDP-glucose synthase) (DTDP-glucose pyrophosphorylase) (Sugar-nucleotidylation enzyme) OS=Frankia alni (strain ACN14a) OX=326424 GN=FRAAL1228 PE=4 SV=1
MM1 pKa = 7.45SLDD4 pKa = 3.76PLLLEE9 pKa = 5.35ILACPCSKK17 pKa = 10.45HH18 pKa = 6.59GEE20 pKa = 3.98LRR22 pKa = 11.84QDD24 pKa = 3.92EE25 pKa = 4.92LDD27 pKa = 3.82GAPVLVCLACDD38 pKa = 3.26LAFPVRR44 pKa = 11.84DD45 pKa = 5.29DD46 pKa = 3.83IPVMLLDD53 pKa = 3.69EE54 pKa = 4.92AVPFSGQAASASSS67 pKa = 3.18

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0RPQ7|Q0RPQ7_FRAAA Hydrogenase expression/formation protein OS=Frankia alni (strain ACN14a) OX=326424 GN=hypD2 PE=3 SV=1
MM1 pKa = 7.23PQRR4 pKa = 11.84AAVRR8 pKa = 11.84APAAGPIRR16 pKa = 11.84PPAVVLLSAVVLLLAVLLLAVRR38 pKa = 11.84LPAVRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84IPRR49 pKa = 11.84VPRR52 pKa = 11.84RR53 pKa = 11.84TRR55 pKa = 11.84APGRR59 pKa = 11.84IRR61 pKa = 3.8

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6677

33

6710

2157716

20

7085

321.6

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.753 ± 0.051

0.766 ± 0.007

6.17 ± 0.027

4.761 ± 0.026

2.554 ± 0.016

10.029 ± 0.039

2.186 ± 0.014

3.217 ± 0.02

1.222 ± 0.017

10.099 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.514 ± 0.01

1.504 ± 0.014

6.924 ± 0.037

2.523 ± 0.019

8.914 ± 0.034

5.109 ± 0.024

5.965 ± 0.028

8.621 ± 0.032

1.416 ± 0.013

1.752 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski