Odoribacter splanchnicus (strain ATCC 29572 / DSM 20712 / CIP 104287 / JCM 15291 / NCTC 10825 / 1651/6) (Bacteroides splanchnicus)
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3479 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9Z6V0|F9Z6V0_ODOSD HipA domain protein OS=Odoribacter splanchnicus (strain ATCC 29572 / DSM 20712 / CIP 104287 / JCM 15291 / NCTC 10825 / 1651/6) OX=709991 GN=Odosp_1810 PE=3 SV=1
MM1 pKa = 7.39 FLNANVCNYY10 pKa = 10.46 DD11 pKa = 3.51 GEE13 pKa = 4.45 DD14 pKa = 3.66 DD15 pKa = 3.75 RR16 pKa = 11.84 WICVEE21 pKa = 3.83 AVTDD25 pKa = 3.78 RR26 pKa = 11.84 DD27 pKa = 3.81 EE28 pKa = 4.58 YY29 pKa = 11.21 EE30 pKa = 4.13 STKK33 pKa = 9.88 FTII36 pKa = 4.21
Molecular weight: 4.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.961
IPC_protein 3.808
Toseland 3.617
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.838
Protein with the highest isoelectric point:
>tr|F9ZB08|F9ZB08_ODOSD 30S ribosomal protein S3 OS=Odoribacter splanchnicus (strain ATCC 29572 / DSM 20712 / CIP 104287 / JCM 15291 / NCTC 10825 / 1651/6) OX=709991 GN=rpsC PE=3 SV=1
MM1 pKa = 7.92 DD2 pKa = 6.15 RR3 pKa = 11.84 DD4 pKa = 3.87 TLIFIAFCVFILLLGVRR21 pKa = 11.84 FWQQLRR27 pKa = 11.84 EE28 pKa = 4.02 RR29 pKa = 11.84 TEE31 pKa = 3.59 RR32 pKa = 11.84 TEE34 pKa = 3.96 ADD36 pKa = 3.3 RR37 pKa = 11.84 SIADD41 pKa = 3.59 EE42 pKa = 4.39 AVPLPAKK49 pKa = 9.83 SRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 YY54 pKa = 9.61 FILIQVIVLGGLLIYY69 pKa = 9.49 MLSWLIRR76 pKa = 11.84 DD77 pKa = 4.39 FKK79 pKa = 11.05 GAEE82 pKa = 3.87 PVLNAGFILRR92 pKa = 11.84 CLIFVFTIYY101 pKa = 10.48 IFISRR106 pKa = 11.84 GRR108 pKa = 11.84 DD109 pKa = 3.32 LLRR112 pKa = 11.84 QRR114 pKa = 11.84 VKK116 pKa = 11.09 GNKK119 pKa = 9.01 KK120 pKa = 9.8 KK121 pKa = 10.88
Molecular weight: 14.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.589
IPC_protein 10.394
Toseland 10.643
ProMoST 10.409
Dawson 10.73
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 10.921
Grimsley 10.789
Solomon 10.862
Lehninger 10.833
Nozaki 10.628
DTASelect 10.438
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 10.672
IPC_peptide 10.862
IPC2_peptide 9.502
IPC2.peptide.svr19 8.648
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3479
0
3479
1258135
30
2242
361.6
40.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.833 ± 0.041
1.234 ± 0.015
5.392 ± 0.028
6.842 ± 0.04
4.699 ± 0.029
6.879 ± 0.037
1.78 ± 0.016
7.057 ± 0.033
6.587 ± 0.036
9.446 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.592 ± 0.017
4.872 ± 0.031
3.685 ± 0.023
3.534 ± 0.022
4.977 ± 0.032
5.811 ± 0.032
5.474 ± 0.035
6.567 ± 0.034
1.246 ± 0.014
4.493 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here