Bacillus phage Spock

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Bequatrovirus; Bacillus virus Spock

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 280 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5PXP5|U5PXP5_9CAUD Thioredoxin OS=Bacillus phage Spock OX=1406791 GN=Spock_131 PE=4 SV=1
MM1 pKa = 7.17YY2 pKa = 10.23SWNNEE7 pKa = 3.64DD8 pKa = 5.14FEE10 pKa = 4.72FEE12 pKa = 4.19EE13 pKa = 4.78LVGKK17 pKa = 8.25TFTRR21 pKa = 11.84IKK23 pKa = 10.72NEE25 pKa = 3.81RR26 pKa = 11.84SDD28 pKa = 3.81EE29 pKa = 4.07LVFYY33 pKa = 9.4DD34 pKa = 4.02TDD36 pKa = 4.01GEE38 pKa = 4.54IYY40 pKa = 11.1VMYY43 pKa = 9.82HH44 pKa = 5.33QQDD47 pKa = 3.89CCEE50 pKa = 3.9SVYY53 pKa = 10.66IEE55 pKa = 6.05DD56 pKa = 4.75IDD58 pKa = 5.92GDD60 pKa = 4.63LDD62 pKa = 4.28DD63 pKa = 5.67LLNSPILMAEE73 pKa = 4.17AVSEE77 pKa = 4.26SGNDD81 pKa = 3.17SDD83 pKa = 5.59YY84 pKa = 11.06GTSTWTFYY92 pKa = 11.03KK93 pKa = 10.27IATIKK98 pKa = 10.04GTVTLRR104 pKa = 11.84WLGEE108 pKa = 4.07SNGYY112 pKa = 9.78YY113 pKa = 9.83SEE115 pKa = 4.79SVDD118 pKa = 3.9FVRR121 pKa = 11.84VTDD124 pKa = 4.05EE125 pKa = 3.8EE126 pKa = 4.49DD127 pKa = 3.1KK128 pKa = 11.1RR129 pKa = 11.84SYY131 pKa = 10.71QEE133 pKa = 3.82NNN135 pKa = 2.88

Molecular weight: 15.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5Q147|U5Q147_9CAUD Uncharacterized protein OS=Bacillus phage Spock OX=1406791 GN=Spock_277 PE=4 SV=1
MM1 pKa = 7.21NVNRR5 pKa = 11.84FYY7 pKa = 10.6IIHH10 pKa = 6.16EE11 pKa = 4.05LARR14 pKa = 11.84VKK16 pKa = 10.46LHH18 pKa = 6.15YY19 pKa = 10.69GGIKK23 pKa = 9.31QRR25 pKa = 11.84HH26 pKa = 5.01LEE28 pKa = 3.9RR29 pKa = 11.84RR30 pKa = 11.84AKK32 pKa = 10.03QLEE35 pKa = 4.4TTPKK39 pKa = 10.47NLMIAVNRR47 pKa = 11.84YY48 pKa = 4.91ITKK51 pKa = 10.24HH52 pKa = 5.62NGVII56 pKa = 3.63

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

280

0

280

49463

23

2033

176.7

20.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.948 ± 0.212

0.851 ± 0.063

6.354 ± 0.124

8.542 ± 0.307

3.94 ± 0.106

6.397 ± 0.218

1.807 ± 0.087

6.486 ± 0.125

8.238 ± 0.19

8.109 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.877 ± 0.08

5.335 ± 0.119

2.903 ± 0.138

3.378 ± 0.166

4.296 ± 0.096

5.384 ± 0.17

6.219 ± 0.206

6.939 ± 0.171

1.237 ± 0.073

4.759 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski