Amino acid dipepetide frequency for Bacillus phage Spock

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.194AlaAla: 3.194 ± 0.44
0.505AlaCys: 0.505 ± 0.117
3.639AlaAsp: 3.639 ± 0.265
4.266AlaGlu: 4.266 ± 0.316
2.527AlaPhe: 2.527 ± 0.243
3.538AlaGly: 3.538 ± 0.45
1.031AlaHis: 1.031 ± 0.133
3.558AlaIle: 3.558 ± 0.265
4.893AlaLys: 4.893 ± 0.318
5.519AlaLeu: 5.519 ± 0.344
1.799AlaMet: 1.799 ± 0.218
3.033AlaAsn: 3.033 ± 0.338
2.406AlaPro: 2.406 ± 0.27
2.345AlaGln: 2.345 ± 0.219
2.648AlaArg: 2.648 ± 0.211
3.376AlaSer: 3.376 ± 0.314
3.963AlaThr: 3.963 ± 0.357
4.003AlaVal: 4.003 ± 0.268
0.586AlaTrp: 0.586 ± 0.103
2.648AlaTyr: 2.648 ± 0.223
0.0AlaXaa: 0.0 ± 0.0
Cys
0.344CysAla: 0.344 ± 0.091
0.162CysCys: 0.162 ± 0.051
0.647CysAsp: 0.647 ± 0.136
0.708CysGlu: 0.708 ± 0.128
0.303CysPhe: 0.303 ± 0.069
0.728CysGly: 0.728 ± 0.149
0.182CysHis: 0.182 ± 0.057
0.546CysIle: 0.546 ± 0.116
0.728CysLys: 0.728 ± 0.13
0.505CysLeu: 0.505 ± 0.103
0.283CysMet: 0.283 ± 0.066
0.445CysAsn: 0.445 ± 0.107
0.303CysPro: 0.303 ± 0.071
0.243CysGln: 0.243 ± 0.073
0.344CysArg: 0.344 ± 0.09
0.425CysSer: 0.425 ± 0.103
0.425CysThr: 0.425 ± 0.082
0.586CysVal: 0.586 ± 0.115
0.142CysTrp: 0.142 ± 0.054
0.465CysTyr: 0.465 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
3.659AspAla: 3.659 ± 0.269
0.546AspCys: 0.546 ± 0.099
3.599AspAsp: 3.599 ± 0.277
5.58AspGlu: 5.58 ± 0.394
3.114AspPhe: 3.114 ± 0.252
4.327AspGly: 4.327 ± 0.404
0.627AspHis: 0.627 ± 0.119
5.155AspIle: 5.155 ± 0.328
5.823AspLys: 5.823 ± 0.312
4.913AspLeu: 4.913 ± 0.281
1.86AspMet: 1.86 ± 0.212
3.356AspAsn: 3.356 ± 0.222
1.415AspPro: 1.415 ± 0.25
0.91AspGln: 0.91 ± 0.143
2.77AspArg: 2.77 ± 0.228
2.952AspSer: 2.952 ± 0.197
3.477AspThr: 3.477 ± 0.266
4.731AspVal: 4.731 ± 0.321
0.869AspTrp: 0.869 ± 0.118
3.841AspTyr: 3.841 ± 0.272
0.0AspXaa: 0.0 ± 0.0
Glu
4.549GluAla: 4.549 ± 0.317
0.546GluCys: 0.546 ± 0.114
5.196GluAsp: 5.196 ± 0.356
9.846GluGlu: 9.846 ± 0.972
3.437GluPhe: 3.437 ± 0.268
4.974GluGly: 4.974 ± 0.264
1.456GluHis: 1.456 ± 0.224
5.58GluIle: 5.58 ± 0.386
6.591GluLys: 6.591 ± 0.457
8.977GluLeu: 8.977 ± 0.59
2.588GluMet: 2.588 ± 0.269
3.882GluAsn: 3.882 ± 0.276
1.88GluPro: 1.88 ± 0.248
3.215GluGln: 3.215 ± 0.29
3.397GluArg: 3.397 ± 0.286
3.983GluSer: 3.983 ± 0.344
3.68GluThr: 3.68 ± 0.274
6.085GluVal: 6.085 ± 0.502
1.375GluTrp: 1.375 ± 0.191
4.185GluTyr: 4.185 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
2.244PheAla: 2.244 ± 0.207
0.586PheCys: 0.586 ± 0.108
2.851PheAsp: 2.851 ± 0.245
2.992PheGlu: 2.992 ± 0.246
1.031PhePhe: 1.031 ± 0.166
2.042PheGly: 2.042 ± 0.243
0.647PheHis: 0.647 ± 0.11
2.527PheIle: 2.527 ± 0.228
3.437PheLys: 3.437 ± 0.289
3.295PheLeu: 3.295 ± 0.241
1.152PheMet: 1.152 ± 0.145
2.163PheAsn: 2.163 ± 0.206
1.355PhePro: 1.355 ± 0.163
1.092PheGln: 1.092 ± 0.17
1.496PheArg: 1.496 ± 0.166
2.83PheSer: 2.83 ± 0.25
2.79PheThr: 2.79 ± 0.265
2.709PheVal: 2.709 ± 0.238
0.384PheTrp: 0.384 ± 0.107
1.779PheTyr: 1.779 ± 0.216
0.0PheXaa: 0.0 ± 0.0
Gly
4.124GlyAla: 4.124 ± 0.474
0.667GlyCys: 0.667 ± 0.131
3.983GlyAsp: 3.983 ± 0.28
4.933GlyGlu: 4.933 ± 0.335
2.911GlyPhe: 2.911 ± 0.277
5.418GlyGly: 5.418 ± 0.882
1.375GlyHis: 1.375 ± 0.168
4.286GlyIle: 4.286 ± 0.358
5.439GlyLys: 5.439 ± 0.335
4.549GlyLeu: 4.549 ± 0.323
1.88GlyMet: 1.88 ± 0.253
3.255GlyAsn: 3.255 ± 0.331
0.0GlyPro: 0.0 ± 0.0
1.981GlyGln: 1.981 ± 0.322
2.487GlyArg: 2.487 ± 0.23
3.74GlySer: 3.74 ± 0.339
3.841GlyThr: 3.841 ± 0.391
4.589GlyVal: 4.589 ± 0.355
1.152GlyTrp: 1.152 ± 0.157
3.356GlyTyr: 3.356 ± 0.289
0.0GlyXaa: 0.0 ± 0.0
His
1.031HisAla: 1.031 ± 0.153
0.182HisCys: 0.182 ± 0.06
1.193HisAsp: 1.193 ± 0.14
1.274HisGlu: 1.274 ± 0.18
0.849HisPhe: 0.849 ± 0.133
0.91HisGly: 0.91 ± 0.126
0.404HisHis: 0.404 ± 0.101
1.193HisIle: 1.193 ± 0.173
1.314HisLys: 1.314 ± 0.17
1.638HisLeu: 1.638 ± 0.202
0.425HisMet: 0.425 ± 0.093
1.152HisAsn: 1.152 ± 0.156
0.667HisPro: 0.667 ± 0.119
0.586HisGln: 0.586 ± 0.097
0.809HisArg: 0.809 ± 0.126
0.708HisSer: 0.708 ± 0.135
1.314HisThr: 1.314 ± 0.178
1.435HisVal: 1.435 ± 0.207
0.243HisTrp: 0.243 ± 0.078
0.748HisTyr: 0.748 ± 0.133
0.0HisXaa: 0.0 ± 0.0
Ile
4.468IleAla: 4.468 ± 0.336
0.465IleCys: 0.465 ± 0.099
5.176IleAsp: 5.176 ± 0.349
5.661IleGlu: 5.661 ± 0.392
1.86IlePhe: 1.86 ± 0.227
3.558IleGly: 3.558 ± 0.278
1.152IleHis: 1.152 ± 0.193
4.003IleIle: 4.003 ± 0.326
6.085IleLys: 6.085 ± 0.367
4.731IleLeu: 4.731 ± 0.282
1.739IleMet: 1.739 ± 0.198
3.841IleAsn: 3.841 ± 0.304
2.224IlePro: 2.224 ± 0.218
1.86IleGln: 1.86 ± 0.191
2.891IleArg: 2.891 ± 0.242
3.599IleSer: 3.599 ± 0.239
4.589IleThr: 4.589 ± 0.359
3.963IleVal: 3.963 ± 0.254
0.445IleTrp: 0.445 ± 0.078
2.547IleTyr: 2.547 ± 0.202
0.0IleXaa: 0.0 ± 0.0
Lys
5.58LysAla: 5.58 ± 0.332
0.687LysCys: 0.687 ± 0.149
5.034LysAsp: 5.034 ± 0.28
8.512LysGlu: 8.512 ± 0.586
2.588LysPhe: 2.588 ± 0.213
5.661LysGly: 5.661 ± 0.403
1.334LysHis: 1.334 ± 0.157
4.266LysIle: 4.266 ± 0.277
6.874LysLys: 6.874 ± 0.497
6.348LysLeu: 6.348 ± 0.393
2.79LysMet: 2.79 ± 0.207
4.084LysAsn: 4.084 ± 0.285
2.608LysPro: 2.608 ± 0.267
3.397LysGln: 3.397 ± 0.264
3.477LysArg: 3.477 ± 0.289
4.044LysSer: 4.044 ± 0.298
4.165LysThr: 4.165 ± 0.3
6.085LysVal: 6.085 ± 0.394
1.092LysTrp: 1.092 ± 0.19
3.76LysTyr: 3.76 ± 0.353
0.0LysXaa: 0.0 ± 0.0
Leu
4.67LeuAla: 4.67 ± 0.326
0.708LeuCys: 0.708 ± 0.13
5.6LeuAsp: 5.6 ± 0.329
7.197LeuGlu: 7.197 ± 0.511
3.134LeuPhe: 3.134 ± 0.262
5.297LeuGly: 5.297 ± 0.32
1.658LeuHis: 1.658 ± 0.188
4.792LeuIle: 4.792 ± 0.339
6.065LeuLys: 6.065 ± 0.36
5.782LeuLeu: 5.782 ± 0.434
2.163LeuMet: 2.163 ± 0.229
4.084LeuAsn: 4.084 ± 0.266
2.77LeuPro: 2.77 ± 0.215
2.81LeuGln: 2.81 ± 0.308
3.801LeuArg: 3.801 ± 0.351
4.731LeuSer: 4.731 ± 0.313
5.661LeuThr: 5.661 ± 0.288
5.802LeuVal: 5.802 ± 0.396
0.768LeuTrp: 0.768 ± 0.112
3.599LeuTyr: 3.599 ± 0.259
0.0LeuXaa: 0.0 ± 0.0
Met
1.921MetAla: 1.921 ± 0.19
0.182MetCys: 0.182 ± 0.068
1.375MetAsp: 1.375 ± 0.175
2.648MetGlu: 2.648 ± 0.264
1.253MetPhe: 1.253 ± 0.164
1.476MetGly: 1.476 ± 0.162
0.505MetHis: 0.505 ± 0.101
1.638MetIle: 1.638 ± 0.191
2.871MetLys: 2.871 ± 0.245
2.183MetLeu: 2.183 ± 0.191
0.627MetMet: 0.627 ± 0.106
1.799MetAsn: 1.799 ± 0.205
0.586MetPro: 0.586 ± 0.098
1.051MetGln: 1.051 ± 0.157
1.395MetArg: 1.395 ± 0.173
1.799MetSer: 1.799 ± 0.183
2.224MetThr: 2.224 ± 0.21
1.577MetVal: 1.577 ± 0.179
0.364MetTrp: 0.364 ± 0.091
1.294MetTyr: 1.294 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
3.033AsnAla: 3.033 ± 0.277
0.384AsnCys: 0.384 ± 0.088
2.992AsnAsp: 2.992 ± 0.196
3.72AsnGlu: 3.72 ± 0.296
1.658AsnPhe: 1.658 ± 0.208
4.266AsnGly: 4.266 ± 0.35
1.233AsnHis: 1.233 ± 0.124
3.154AsnIle: 3.154 ± 0.255
4.812AsnLys: 4.812 ± 0.292
4.023AsnLeu: 4.023 ± 0.307
1.961AsnMet: 1.961 ± 0.195
3.093AsnAsn: 3.093 ± 0.286
2.022AsnPro: 2.022 ± 0.276
1.779AsnGln: 1.779 ± 0.191
2.729AsnArg: 2.729 ± 0.255
2.406AsnSer: 2.406 ± 0.295
3.154AsnThr: 3.154 ± 0.339
3.801AsnVal: 3.801 ± 0.246
0.687AsnTrp: 0.687 ± 0.138
2.446AsnTyr: 2.446 ± 0.216
0.0AsnXaa: 0.0 ± 0.0
Pro
1.9ProAla: 1.9 ± 0.285
0.222ProCys: 0.222 ± 0.068
1.799ProAsp: 1.799 ± 0.197
2.709ProGlu: 2.709 ± 0.256
1.537ProPhe: 1.537 ± 0.191
0.728ProGly: 0.728 ± 0.115
0.586ProHis: 0.586 ± 0.104
1.759ProIle: 1.759 ± 0.199
1.9ProLys: 1.9 ± 0.204
2.163ProLeu: 2.163 ± 0.215
0.809ProMet: 0.809 ± 0.135
1.516ProAsn: 1.516 ± 0.162
0.89ProPro: 0.89 ± 0.189
1.132ProGln: 1.132 ± 0.23
1.253ProArg: 1.253 ± 0.138
1.9ProSer: 1.9 ± 0.236
2.123ProThr: 2.123 ± 0.213
2.264ProVal: 2.264 ± 0.209
0.243ProTrp: 0.243 ± 0.071
1.597ProTyr: 1.597 ± 0.183
0.0ProXaa: 0.0 ± 0.0
Gln
2.83GlnAla: 2.83 ± 0.35
0.263GlnCys: 0.263 ± 0.063
2.022GlnAsp: 2.022 ± 0.174
2.83GlnGlu: 2.83 ± 0.258
1.193GlnPhe: 1.193 ± 0.169
2.285GlnGly: 2.285 ± 0.26
0.687GlnHis: 0.687 ± 0.125
1.88GlnIle: 1.88 ± 0.18
2.264GlnLys: 2.264 ± 0.21
3.012GlnLeu: 3.012 ± 0.265
0.991GlnMet: 0.991 ± 0.138
1.698GlnAsn: 1.698 ± 0.185
0.991GlnPro: 0.991 ± 0.16
1.638GlnGln: 1.638 ± 0.308
1.435GlnArg: 1.435 ± 0.162
1.9GlnSer: 1.9 ± 0.219
2.082GlnThr: 2.082 ± 0.24
2.002GlnVal: 2.002 ± 0.201
0.445GlnTrp: 0.445 ± 0.095
1.334GlnTyr: 1.334 ± 0.121
0.0GlnXaa: 0.0 ± 0.0
Arg
2.345ArgAla: 2.345 ± 0.192
0.303ArgCys: 0.303 ± 0.08
2.709ArgAsp: 2.709 ± 0.261
4.044ArgGlu: 4.044 ± 0.323
2.143ArgPhe: 2.143 ± 0.208
3.174ArgGly: 3.174 ± 0.225
0.768ArgHis: 0.768 ± 0.141
2.992ArgIle: 2.992 ± 0.276
3.033ArgLys: 3.033 ± 0.301
3.963ArgLeu: 3.963 ± 0.319
1.355ArgMet: 1.355 ± 0.163
2.022ArgAsn: 2.022 ± 0.183
1.051ArgPro: 1.051 ± 0.129
1.597ArgGln: 1.597 ± 0.186
1.658ArgArg: 1.658 ± 0.162
1.86ArgSer: 1.86 ± 0.228
2.305ArgThr: 2.305 ± 0.201
2.952ArgVal: 2.952 ± 0.257
0.566ArgTrp: 0.566 ± 0.121
2.123ArgTyr: 2.123 ± 0.221
0.0ArgXaa: 0.0 ± 0.0
Ser
3.275SerAla: 3.275 ± 0.337
0.303SerCys: 0.303 ± 0.083
3.033SerAsp: 3.033 ± 0.272
3.821SerGlu: 3.821 ± 0.283
2.608SerPhe: 2.608 ± 0.237
3.579SerGly: 3.579 ± 0.315
1.132SerHis: 1.132 ± 0.187
4.185SerIle: 4.185 ± 0.326
4.428SerLys: 4.428 ± 0.301
4.306SerLeu: 4.306 ± 0.282
1.638SerMet: 1.638 ± 0.181
2.952SerAsn: 2.952 ± 0.258
1.557SerPro: 1.557 ± 0.193
1.253SerGln: 1.253 ± 0.173
2.204SerArg: 2.204 ± 0.221
3.134SerSer: 3.134 ± 0.324
3.477SerThr: 3.477 ± 0.355
3.295SerVal: 3.295 ± 0.294
0.91SerTrp: 0.91 ± 0.151
2.75SerTyr: 2.75 ± 0.245
0.0SerXaa: 0.0 ± 0.0
Thr
3.376ThrAla: 3.376 ± 0.288
0.627ThrCys: 0.627 ± 0.11
3.336ThrAsp: 3.336 ± 0.308
4.124ThrGlu: 4.124 ± 0.258
2.426ThrPhe: 2.426 ± 0.234
4.327ThrGly: 4.327 ± 0.258
0.809ThrHis: 0.809 ± 0.14
4.266ThrIle: 4.266 ± 0.309
4.812ThrLys: 4.812 ± 0.318
5.439ThrLeu: 5.439 ± 0.361
1.456ThrMet: 1.456 ± 0.177
3.841ThrAsn: 3.841 ± 0.336
2.467ThrPro: 2.467 ± 0.28
2.204ThrGln: 2.204 ± 0.206
2.689ThrArg: 2.689 ± 0.258
3.316ThrSer: 3.316 ± 0.261
3.538ThrThr: 3.538 ± 0.301
4.994ThrVal: 4.994 ± 0.4
0.748ThrTrp: 0.748 ± 0.14
3.397ThrTyr: 3.397 ± 0.279
0.0ThrXaa: 0.0 ± 0.0
Val
3.882ValAla: 3.882 ± 0.292
0.586ValCys: 0.586 ± 0.116
4.852ValAsp: 4.852 ± 0.275
5.802ValGlu: 5.802 ± 0.446
2.83ValPhe: 2.83 ± 0.232
4.064ValGly: 4.064 ± 0.295
1.314ValHis: 1.314 ± 0.167
4.569ValIle: 4.569 ± 0.281
6.065ValLys: 6.065 ± 0.388
5.196ValLeu: 5.196 ± 0.325
1.597ValMet: 1.597 ± 0.186
3.579ValAsn: 3.579 ± 0.355
2.386ValPro: 2.386 ± 0.239
2.365ValGln: 2.365 ± 0.198
2.669ValArg: 2.669 ± 0.236
3.882ValSer: 3.882 ± 0.33
5.499ValThr: 5.499 ± 0.364
4.69ValVal: 4.69 ± 0.29
0.627ValTrp: 0.627 ± 0.094
2.932ValTyr: 2.932 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
0.526TrpAla: 0.526 ± 0.112
0.162TrpCys: 0.162 ± 0.061
0.93TrpAsp: 0.93 ± 0.129
0.97TrpGlu: 0.97 ± 0.16
0.526TrpPhe: 0.526 ± 0.099
0.748TrpGly: 0.748 ± 0.126
0.303TrpHis: 0.303 ± 0.07
0.93TrpIle: 0.93 ± 0.127
0.829TrpLys: 0.829 ± 0.12
0.93TrpLeu: 0.93 ± 0.142
0.485TrpMet: 0.485 ± 0.093
0.667TrpAsn: 0.667 ± 0.153
0.0TrpPro: 0.0 ± 0.0
0.364TrpGln: 0.364 ± 0.103
0.505TrpArg: 0.505 ± 0.098
0.849TrpSer: 0.849 ± 0.159
0.768TrpThr: 0.768 ± 0.143
0.91TrpVal: 0.91 ± 0.154
0.222TrpTrp: 0.222 ± 0.058
0.748TrpTyr: 0.748 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.527TyrAla: 2.527 ± 0.186
0.425TyrCys: 0.425 ± 0.102
3.579TyrAsp: 3.579 ± 0.269
3.781TyrGlu: 3.781 ± 0.301
1.476TyrPhe: 1.476 ± 0.163
2.871TyrGly: 2.871 ± 0.263
0.869TyrHis: 0.869 ± 0.125
3.579TyrIle: 3.579 ± 0.281
4.124TyrLys: 4.124 ± 0.348
3.619TyrLeu: 3.619 ± 0.287
1.152TyrMet: 1.152 ± 0.138
2.891TyrAsn: 2.891 ± 0.226
1.456TyrPro: 1.456 ± 0.149
1.961TyrGln: 1.961 ± 0.249
2.305TyrArg: 2.305 ± 0.212
2.406TyrSer: 2.406 ± 0.249
3.114TyrThr: 3.114 ± 0.243
2.911TyrVal: 2.911 ± 0.254
0.505TyrTrp: 0.505 ± 0.104
2.042TyrTyr: 2.042 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 280 proteins (49463 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski