candidate division MSBL1 archaeon SCGC-AAA259E22

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133UID5|A0A133UID5_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259E22 OX=1698265 GN=AKJ66_00355 PE=4 SV=1
MM1 pKa = 7.51TEE3 pKa = 4.19VICAVPGCEE12 pKa = 4.75NNLTDD17 pKa = 3.76EE18 pKa = 4.07QRR20 pKa = 11.84EE21 pKa = 4.12NKK23 pKa = 9.94IPVCSEE29 pKa = 3.67CEE31 pKa = 3.71AANMHH36 pKa = 5.76VCEE39 pKa = 4.51SCNKK43 pKa = 9.86RR44 pKa = 11.84LSTEE48 pKa = 3.91QIQNGGSLCSEE59 pKa = 4.61CEE61 pKa = 4.15GNPSGLEE68 pKa = 3.81EE69 pKa = 4.35SMEE72 pKa = 4.4EE73 pKa = 4.01YY74 pKa = 10.79GEE76 pKa = 4.15EE77 pKa = 4.37YY78 pKa = 10.64IMGTDD83 pKa = 3.43TEE85 pKa = 4.67EE86 pKa = 3.79EE87 pKa = 4.45GFMVGEE93 pKa = 4.32SKK95 pKa = 10.67EE96 pKa = 5.11DD97 pKa = 3.44EE98 pKa = 4.35DD99 pKa = 5.95FMVV102 pKa = 3.97

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133UH94|A0A133UH94_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259E22 OX=1698265 GN=AKJ66_01770 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.2PDD4 pKa = 3.33KK5 pKa = 10.89AIIEE9 pKa = 4.26PQSEE13 pKa = 4.43NKK15 pKa = 10.49GKK17 pKa = 8.65ITDD20 pKa = 3.56KK21 pKa = 10.69RR22 pKa = 11.84KK23 pKa = 10.28SPLTIGGWGSWKK35 pKa = 9.17MRR37 pKa = 11.84SRR39 pKa = 11.84RR40 pKa = 11.84NGKK43 pKa = 9.24PSGNSEE49 pKa = 3.66GRR51 pKa = 11.84RR52 pKa = 11.84KK53 pKa = 9.9RR54 pKa = 11.84KK55 pKa = 9.7RR56 pKa = 11.84ISRR59 pKa = 11.84RR60 pKa = 11.84ISSSRR65 pKa = 11.84ASSGRR70 pKa = 11.84GFRR73 pKa = 11.84KK74 pKa = 9.5PNRR77 pKa = 11.84LLRR80 pKa = 11.84RR81 pKa = 11.84YY82 pKa = 9.78SSRR85 pKa = 11.84KK86 pKa = 9.2YY87 pKa = 9.47PRR89 pKa = 11.84QLAGSSGSYY98 pKa = 10.09EE99 pKa = 4.22GGSQSLTWTKK109 pKa = 10.76DD110 pKa = 3.22YY111 pKa = 11.17QIVVRR116 pKa = 11.84DD117 pKa = 3.94GEE119 pKa = 4.3EE120 pKa = 3.38EE121 pKa = 4.15KK122 pKa = 11.04AFHH125 pKa = 6.64KK126 pKa = 10.8LSGGEE131 pKa = 4.0QVCAALTVRR140 pKa = 11.84LAILQRR146 pKa = 11.84LAPINFAFLDD156 pKa = 4.23EE157 pKa = 4.29PTEE160 pKa = 4.1NLDD163 pKa = 3.19EE164 pKa = 4.61ARR166 pKa = 11.84RR167 pKa = 11.84QNLSSRR173 pKa = 11.84LEE175 pKa = 4.14SIKK178 pKa = 10.94ALDD181 pKa = 3.61QLCVISHH188 pKa = 6.53DD189 pKa = 3.68RR190 pKa = 11.84VFEE193 pKa = 4.52GIADD197 pKa = 4.0RR198 pKa = 11.84TISLEE203 pKa = 3.96KK204 pKa = 10.69KK205 pKa = 8.25EE206 pKa = 4.15GKK208 pKa = 9.99NGGSGASPTKK218 pKa = 9.42ITSSTAGQQPRR229 pKa = 11.84VEE231 pKa = 4.1RR232 pKa = 11.84SEE234 pKa = 3.92GSPRR238 pKa = 11.84RR239 pKa = 11.84GLRR242 pKa = 11.84RR243 pKa = 11.84LDD245 pKa = 3.33YY246 pKa = 10.72LRR248 pKa = 11.84RR249 pKa = 11.84AEE251 pKa = 4.78CIPGVFSTSRR261 pKa = 11.84PAA263 pKa = 3.63

Molecular weight: 29.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

843

0

843

191784

39

1234

227.5

25.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.973 ± 0.084

1.076 ± 0.041

5.792 ± 0.081

10.035 ± 0.127

4.073 ± 0.083

7.524 ± 0.093

1.646 ± 0.04

7.021 ± 0.085

7.573 ± 0.099

9.066 ± 0.104

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.244 ± 0.041

3.853 ± 0.059

4.03 ± 0.063

2.216 ± 0.04

5.743 ± 0.095

6.362 ± 0.074

4.751 ± 0.07

6.897 ± 0.079

1.122 ± 0.036

3.004 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski