Eubacterium coprostanoligenes
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1647 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4JVP7|A0A1T4JVP7_9FIRM Cobalt/nickel transport system permease protein OS=Eubacterium coprostanoligenes OX=290054 GN=SAMN02745114_00137 PE=4 SV=1
MM1 pKa = 7.52 NIKK4 pKa = 9.84 IDD6 pKa = 3.71 VDD8 pKa = 3.61 NLRR11 pKa = 11.84 DD12 pKa = 4.01 DD13 pKa = 4.19 LRR15 pKa = 11.84 DD16 pKa = 3.68 YY17 pKa = 11.03 YY18 pKa = 11.4 GSAVGCNPTAMADD31 pKa = 3.81 VINIDD36 pKa = 3.52 SMSDD40 pKa = 2.96 EE41 pKa = 4.51 DD42 pKa = 4.14 VVEE45 pKa = 4.16 KK46 pKa = 10.79 AVEE49 pKa = 4.04 NGFNLYY55 pKa = 10.42 DD56 pKa = 3.84 YY57 pKa = 10.76 EE58 pKa = 4.38 VV59 pKa = 3.13
Molecular weight: 6.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.668
IPC_protein 3.617
Toseland 3.414
ProMoST 3.834
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.617
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.783
DTASelect 4.024
Thurlkill 3.49
EMBOSS 3.63
Sillero 3.757
Patrickios 1.863
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.71
Protein with the highest isoelectric point:
>tr|A0A1T4M6N3|A0A1T4M6N3_9FIRM VanZ like family protein OS=Eubacterium coprostanoligenes OX=290054 GN=SAMN02745114_01153 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.95 KK9 pKa = 8.27 RR10 pKa = 11.84 HH11 pKa = 4.51 AQKK14 pKa = 10.15 VHH16 pKa = 5.45 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.58 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.07 GRR39 pKa = 11.84 KK40 pKa = 7.8 SLSYY44 pKa = 11.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1647
0
1647
547691
41
3655
332.5
37.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.229 ± 0.064
1.702 ± 0.031
6.346 ± 0.046
6.755 ± 0.056
4.423 ± 0.051
6.767 ± 0.052
1.53 ± 0.026
7.678 ± 0.053
7.949 ± 0.053
8.057 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.691 ± 0.031
5.449 ± 0.054
3.167 ± 0.033
2.654 ± 0.025
3.474 ± 0.037
6.226 ± 0.052
5.673 ± 0.051
7.278 ± 0.05
0.775 ± 0.021
4.176 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here