Amino acid dipepetide frequency for Eubacterium coprostanoligenes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.353AlaAla: 4.353 ± 0.124	1.227AlaCys: 1.227 ± 0.05	4.572AlaAsp: 4.572 ± 0.104	4.868AlaGlu: 4.868 ± 0.111	3.106AlaPhe: 3.106 ± 0.089	4.906AlaGly: 4.906 ± 0.116	1.128AlaHis: 1.128 ± 0.044	5.447AlaIle: 5.447 ± 0.126	5.998AlaLys: 5.998 ± 0.113	6.498AlaLeu: 6.498 ± 0.13	2.096AlaMet: 2.096 ± 0.069	3.237AlaAsn: 3.237 ± 0.085	2.149AlaPro: 2.149 ± 0.073	2.374AlaGln: 2.374 ± 0.061	2.416AlaArg: 2.416 ± 0.074	4.123AlaSer: 4.123 ± 0.09	3.904AlaThr: 3.904 ± 0.096	6.653AlaVal: 6.653 ± 0.138	0.456AlaTrp: 0.456 ± 0.026	2.779AlaTyr: 2.779 ± 0.069	0.0AlaXaa: 0.0 ± 0.0
Cys	1.218CysAla: 1.218 ± 0.049	0.309CysCys: 0.309 ± 0.026	1.251CysAsp: 1.251 ± 0.052	1.119CysGlu: 1.119 ± 0.044	0.787CysPhe: 0.787 ± 0.04	1.632CysGly: 1.632 ± 0.061	0.283CysHis: 0.283 ± 0.026	1.106CysIle: 1.106 ± 0.047	1.227CysLys: 1.227 ± 0.047	1.141CysLeu: 1.141 ± 0.042	0.387CysMet: 0.387 ± 0.023	0.825CysAsn: 0.825 ± 0.045	0.745CysPro: 0.745 ± 0.041	0.398CysGln: 0.398 ± 0.029	0.531CysArg: 0.531 ± 0.032	1.108CysSer: 1.108 ± 0.064	0.946CysThr: 0.946 ± 0.047	1.258CysVal: 1.258 ± 0.046	0.131CysTrp: 0.131 ± 0.016	0.619CysTyr: 0.619 ± 0.033	0.0CysXaa: 0.0 ± 0.0
Asp	4.141AspAla: 4.141 ± 0.085	1.074AspCys: 1.074 ± 0.051	4.141AspAsp: 4.141 ± 0.104	5.709AspGlu: 5.709 ± 0.114	3.31AspPhe: 3.31 ± 0.082	4.959AspGly: 4.959 ± 0.093	0.679AspHis: 0.679 ± 0.034	5.006AspIle: 5.006 ± 0.094	5.008AspLys: 5.008 ± 0.103	4.658AspLeu: 4.658 ± 0.085	1.987AspMet: 1.987 ± 0.059	3.259AspAsn: 3.259 ± 0.097	1.455AspPro: 1.455 ± 0.051	0.811AspGln: 0.811 ± 0.037	2.144AspArg: 2.144 ± 0.058	3.654AspSer: 3.654 ± 0.093	3.268AspThr: 3.268 ± 0.085	4.541AspVal: 4.541 ± 0.092	0.513AspTrp: 0.513 ± 0.035	3.148AspTyr: 3.148 ± 0.079	0.0AspXaa: 0.0 ± 0.0
Glu	4.902GluAla: 4.902 ± 0.098	1.03GluCys: 1.03 ± 0.055	3.413GluAsp: 3.413 ± 0.084	5.09GluGlu: 5.09 ± 0.116	2.92GluPhe: 2.92 ± 0.08	3.358GluGly: 3.358 ± 0.091	1.147GluHis: 1.147 ± 0.048	5.666GluIle: 5.666 ± 0.104	6.193GluLys: 6.193 ± 0.122	6.175GluLeu: 6.175 ± 0.137	2.019GluMet: 2.019 ± 0.069	4.767GluAsn: 4.767 ± 0.1	1.691GluPro: 1.691 ± 0.053	2.397GluGln: 2.397 ± 0.074	2.501GluArg: 2.501 ± 0.082	3.144GluSer: 3.144 ± 0.078	3.036GluThr: 3.036 ± 0.077	4.258GluVal: 4.258 ± 0.1	0.542GluTrp: 0.542 ± 0.04	3.301GluTyr: 3.301 ± 0.083	0.0GluXaa: 0.0 ± 0.0
Phe	3.367PheAla: 3.367 ± 0.092	0.809PheCys: 0.809 ± 0.037	3.518PheAsp: 3.518 ± 0.08	3.157PheGlu: 3.157 ± 0.084	1.884PhePhe: 1.884 ± 0.071	3.498PheGly: 3.498 ± 0.085	0.635PheHis: 0.635 ± 0.032	3.038PheIle: 3.038 ± 0.087	3.013PheLys: 3.013 ± 0.074	3.305PheLeu: 3.305 ± 0.089	1.026PheMet: 1.026 ± 0.048	2.319PheAsn: 2.319 ± 0.062	1.231PhePro: 1.231 ± 0.053	0.825PheGln: 0.825 ± 0.038	1.358PheArg: 1.358 ± 0.051	3.098PheSer: 3.098 ± 0.084	2.536PheThr: 2.536 ± 0.078	3.478PheVal: 3.478 ± 0.085	0.312PheTrp: 0.312 ± 0.024	1.824PheTyr: 1.824 ± 0.066	0.0PheXaa: 0.0 ± 0.0
Gly	4.902GlyAla: 4.902 ± 0.11	1.262GlyCys: 1.262 ± 0.049	3.979GlyAsp: 3.979 ± 0.078	4.439GlyGlu: 4.439 ± 0.099	3.203GlyPhe: 3.203 ± 0.075	4.713GlyGly: 4.713 ± 0.126	1.136GlyHis: 1.136 ± 0.054	5.649GlyIle: 5.649 ± 0.113	5.757GlyLys: 5.757 ± 0.111	5.171GlyLeu: 5.171 ± 0.112	1.897GlyMet: 1.897 ± 0.059	3.241GlyAsn: 3.241 ± 0.109	1.127GlyPro: 1.127 ± 0.059	1.579GlyGln: 1.579 ± 0.059	2.403GlyArg: 2.403 ± 0.067	3.829GlySer: 3.829 ± 0.105	4.161GlyThr: 4.161 ± 0.104	5.421GlyVal: 5.421 ± 0.111	0.593GlyTrp: 0.593 ± 0.036	3.212GlyTyr: 3.212 ± 0.076	0.0GlyXaa: 0.0 ± 0.0
His	0.907HisAla: 0.907 ± 0.038	0.276HisCys: 0.276 ± 0.021	0.811HisAsp: 0.811 ± 0.041	0.781HisGlu: 0.781 ± 0.039	0.796HisPhe: 0.796 ± 0.041	1.225HisGly: 1.225 ± 0.053	0.402HisHis: 0.402 ± 0.029	1.426HisIle: 1.426 ± 0.048	1.068HisLys: 1.068 ± 0.042	1.136HisLeu: 1.136 ± 0.053	0.343HisMet: 0.343 ± 0.024	0.865HisAsn: 0.865 ± 0.047	0.798HisPro: 0.798 ± 0.043	0.398HisGln: 0.398 ± 0.027	0.635HisArg: 0.635 ± 0.031	1.052HisSer: 1.052 ± 0.041	0.884HisThr: 0.884 ± 0.039	0.63HisVal: 0.63 ± 0.035	0.155HisTrp: 0.155 ± 0.017	0.714HisTyr: 0.714 ± 0.037	0.0HisXaa: 0.0 ± 0.0
Ile	5.901IleAla: 5.901 ± 0.107	1.421IleCys: 1.421 ± 0.054	5.465IleAsp: 5.465 ± 0.108	5.1IleGlu: 5.1 ± 0.105	3.007IlePhe: 3.007 ± 0.083	5.176IleGly: 5.176 ± 0.112	1.028IleHis: 1.028 ± 0.042	6.193IleIle: 6.193 ± 0.138	6.281IleLys: 6.281 ± 0.1	5.658IleLeu: 5.658 ± 0.119	1.912IleMet: 1.912 ± 0.058	4.221IleAsn: 4.221 ± 0.105	2.825IlePro: 2.825 ± 0.085	1.654IleGln: 1.654 ± 0.058	2.516IleArg: 2.516 ± 0.072	5.027IleSer: 5.027 ± 0.109	4.607IleThr: 4.607 ± 0.093	5.563IleVal: 5.563 ± 0.109	0.529IleTrp: 0.529 ± 0.03	2.697IleTyr: 2.697 ± 0.075	0.0IleXaa: 0.0 ± 0.0
Lys	5.939LysAla: 5.939 ± 0.124	1.161LysCys: 1.161 ± 0.049	4.625LysAsp: 4.625 ± 0.086	5.936LysGlu: 5.936 ± 0.11	2.89LysPhe: 2.89 ± 0.075	4.555LysGly: 4.555 ± 0.098	1.143LysHis: 1.143 ± 0.045	6.243LysIle: 6.243 ± 0.111	7.429LysLys: 7.429 ± 0.152	5.682LysLeu: 5.682 ± 0.096	2.31LysMet: 2.31 ± 0.059	5.202LysAsn: 5.202 ± 0.101	2.472LysPro: 2.472 ± 0.063	2.326LysGln: 2.326 ± 0.078	2.843LysArg: 2.843 ± 0.081	4.711LysSer: 4.711 ± 0.092	4.683LysThr: 4.683 ± 0.106	5.043LysVal: 5.043 ± 0.09	0.65LysTrp: 0.65 ± 0.038	3.648LysTyr: 3.648 ± 0.076	0.0LysXaa: 0.0 ± 0.0
Leu	6.051LeuAla: 6.051 ± 0.106	1.556LeuCys: 1.556 ± 0.056	5.251LeuAsp: 5.251 ± 0.098	4.996LeuGlu: 4.996 ± 0.125	3.652LeuPhe: 3.652 ± 0.114	5.56LeuGly: 5.56 ± 0.119	1.147LeuHis: 1.147 ± 0.049	5.715LeuIle: 5.715 ± 0.126	6.255LeuLys: 6.255 ± 0.103	6.695LeuLeu: 6.695 ± 0.151	2.103LeuMet: 2.103 ± 0.066	4.311LeuAsn: 4.311 ± 0.09	3.011LeuPro: 3.011 ± 0.073	2.198LeuGln: 2.198 ± 0.069	2.748LeuArg: 2.748 ± 0.081	5.781LeuSer: 5.781 ± 0.12	4.875LeuThr: 4.875 ± 0.088	5.211LeuVal: 5.211 ± 0.105	0.599LeuTrp: 0.599 ± 0.036	2.859LeuTyr: 2.859 ± 0.083	0.0LeuXaa: 0.0 ± 0.0
Met	2.142MetAla: 2.142 ± 0.07	0.414MetCys: 0.414 ± 0.025	1.488MetAsp: 1.488 ± 0.05	1.428MetGlu: 1.428 ± 0.055	1.169MetPhe: 1.169 ± 0.053	1.837MetGly: 1.837 ± 0.059	0.414MetHis: 0.414 ± 0.028	1.934MetIle: 1.934 ± 0.07	2.248MetLys: 2.248 ± 0.063	2.452MetLeu: 2.452 ± 0.066	0.767MetMet: 0.767 ± 0.039	1.546MetAsn: 1.546 ± 0.055	1.132MetPro: 1.132 ± 0.048	0.803MetGln: 0.803 ± 0.035	1.032MetArg: 1.032 ± 0.047	1.798MetSer: 1.798 ± 0.066	1.579MetThr: 1.579 ± 0.049	1.548MetVal: 1.548 ± 0.058	0.183MetTrp: 0.183 ± 0.02	0.999MetTyr: 0.999 ± 0.043	0.0MetXaa: 0.0 ± 0.0
Asn	3.845AsnAla: 3.845 ± 0.097	0.926AsnCys: 0.926 ± 0.042	3.433AsnAsp: 3.433 ± 0.088	3.759AsnGlu: 3.759 ± 0.082	2.094AsnPhe: 2.094 ± 0.072	4.413AsnGly: 4.413 ± 0.109	0.816AsnHis: 0.816 ± 0.041	4.402AsnIle: 4.402 ± 0.088	3.924AsnLys: 3.924 ± 0.103	4.024AsnLeu: 4.024 ± 0.072	1.428AsnMet: 1.428 ± 0.051	3.082AsnAsn: 3.082 ± 0.094	2.131AsnPro: 2.131 ± 0.06	1.413AsnGln: 1.413 ± 0.052	1.8AsnArg: 1.8 ± 0.059	3.61AsnSer: 3.61 ± 0.102	2.901AsnThr: 2.901 ± 0.08	3.758AsnVal: 3.758 ± 0.097	0.405AsnTrp: 0.405 ± 0.028	2.324AsnTyr: 2.324 ± 0.072	0.0AsnXaa: 0.0 ± 0.0
Pro	2.071ProAla: 2.071 ± 0.071	0.562ProCys: 0.562 ± 0.033	2.147ProAsp: 2.147 ± 0.066	2.461ProGlu: 2.461 ± 0.068	1.561ProPhe: 1.561 ± 0.055	1.588ProGly: 1.588 ± 0.059	0.497ProHis: 0.497 ± 0.033	2.297ProIle: 2.297 ± 0.06	2.284ProLys: 2.284 ± 0.069	2.567ProLeu: 2.567 ± 0.071	0.858ProMet: 0.858 ± 0.037	1.789ProAsn: 1.789 ± 0.066	0.781ProPro: 0.781 ± 0.049	1.079ProGln: 1.079 ± 0.044	0.938ProArg: 0.938 ± 0.043	2.001ProSer: 2.001 ± 0.064	1.866ProThr: 1.866 ± 0.062	2.596ProVal: 2.596 ± 0.072	0.349ProTrp: 0.349 ± 0.026	1.375ProTyr: 1.375 ± 0.05	0.0ProXaa: 0.0 ± 0.0
Gln	1.868GlnAla: 1.868 ± 0.062	0.356GlnCys: 0.356 ± 0.027	1.172GlnAsp: 1.172 ± 0.048	1.546GlnGlu: 1.546 ± 0.054	1.117GlnPhe: 1.117 ± 0.047	1.658GlnGly: 1.658 ± 0.051	0.385GlnHis: 0.385 ± 0.03	2.206GlnIle: 2.206 ± 0.07	2.399GlnLys: 2.399 ± 0.066	2.284GlnLeu: 2.284 ± 0.068	0.869GlnMet: 0.869 ± 0.039	1.784GlnAsn: 1.784 ± 0.051	0.738GlnPro: 0.738 ± 0.043	0.783GlnGln: 0.783 ± 0.049	1.11GlnArg: 1.11 ± 0.046	1.627GlnSer: 1.627 ± 0.056	1.696GlnThr: 1.696 ± 0.06	1.572GlnVal: 1.572 ± 0.052	0.241GlnTrp: 0.241 ± 0.02	1.123GlnTyr: 1.123 ± 0.052	0.0GlnXaa: 0.0 ± 0.0
Arg	2.282ArgAla: 2.282 ± 0.064	0.595ArgCys: 0.595 ± 0.035	1.913ArgAsp: 1.913 ± 0.059	2.613ArgGlu: 2.613 ± 0.073	1.658ArgPhe: 1.658 ± 0.056	2.06ArgGly: 2.06 ± 0.069	0.652ArgHis: 0.652 ± 0.036	2.914ArgIle: 2.914 ± 0.076	2.622ArgLys: 2.622 ± 0.074	3.058ArgLeu: 3.058 ± 0.082	1.055ArgMet: 1.055 ± 0.044	1.66ArgAsn: 1.66 ± 0.057	1.169ArgPro: 1.169 ± 0.046	1.163ArgGln: 1.163 ± 0.047	1.554ArgArg: 1.554 ± 0.065	1.775ArgSer: 1.775 ± 0.057	1.82ArgThr: 1.82 ± 0.057	2.432ArgVal: 2.432 ± 0.072	0.236ArgTrp: 0.236 ± 0.02	1.508ArgTyr: 1.508 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	4.891SerAla: 4.891 ± 0.104	0.864SerCys: 0.864 ± 0.044	3.969SerAsp: 3.969 ± 0.094	3.522SerGlu: 3.522 ± 0.086	2.962SerPhe: 2.962 ± 0.078	4.503SerGly: 4.503 ± 0.106	1.063SerHis: 1.063 ± 0.04	4.455SerIle: 4.455 ± 0.109	4.851SerLys: 4.851 ± 0.113	5.165SerLeu: 5.165 ± 0.109	1.503SerMet: 1.503 ± 0.048	3.192SerAsn: 3.192 ± 0.098	1.798SerPro: 1.798 ± 0.061	1.672SerGln: 1.672 ± 0.054	2.363SerArg: 2.363 ± 0.076	4.136SerSer: 4.136 ± 0.12	3.444SerThr: 3.444 ± 0.088	4.992SerVal: 4.992 ± 0.123	0.502SerTrp: 0.502 ± 0.034	2.416SerTyr: 2.416 ± 0.072	0.0SerXaa: 0.0 ± 0.0
Thr	4.762ThrAla: 4.762 ± 0.104	0.761ThrCys: 0.761 ± 0.037	4.0ThrAsp: 4.0 ± 0.082	3.482ThrGlu: 3.482 ± 0.085	2.417ThrPhe: 2.417 ± 0.061	4.07ThrGly: 4.07 ± 0.096	0.898ThrHis: 0.898 ± 0.042	4.03ThrIle: 4.03 ± 0.112	3.942ThrLys: 3.942 ± 0.085	4.703ThrLeu: 4.703 ± 0.111	1.315ThrMet: 1.315 ± 0.055	2.503ThrAsn: 2.503 ± 0.078	2.275ThrPro: 2.275 ± 0.069	1.442ThrGln: 1.442 ± 0.056	1.609ThrArg: 1.609 ± 0.054	3.38ThrSer: 3.38 ± 0.087	3.31ThrThr: 3.31 ± 0.123	5.235ThrVal: 5.235 ± 0.11	0.394ThrTrp: 0.394 ± 0.027	2.196ThrTyr: 2.196 ± 0.072	0.0ThrXaa: 0.0 ± 0.0
Val	5.594ValAla: 5.594 ± 0.134	1.499ValCys: 1.499 ± 0.054	4.787ValAsp: 4.787 ± 0.111	4.587ValGlu: 4.587 ± 0.112	3.279ValPhe: 3.279 ± 0.088	4.528ValGly: 4.528 ± 0.091	1.033ValHis: 1.033 ± 0.044	5.341ValIle: 5.341 ± 0.108	5.377ValLys: 5.377 ± 0.119	6.317ValLeu: 6.317 ± 0.133	1.901ValMet: 1.901 ± 0.064	3.657ValAsn: 3.657 ± 0.104	2.627ValPro: 2.627 ± 0.073	1.789ValGln: 1.789 ± 0.058	2.489ValArg: 2.489 ± 0.069	4.802ValSer: 4.802 ± 0.098	4.344ValThr: 4.344 ± 0.109	5.571ValVal: 5.571 ± 0.111	0.52ValTrp: 0.52 ± 0.032	2.737ValTyr: 2.737 ± 0.073	0.0ValXaa: 0.0 ± 0.0
Trp	0.528TrpAla: 0.528 ± 0.029	0.183TrpCys: 0.183 ± 0.019	0.498TrpAsp: 0.498 ± 0.033	0.446TrpGlu: 0.446 ± 0.034	0.343TrpPhe: 0.343 ± 0.028	0.606TrpGly: 0.606 ± 0.035	0.128TrpHis: 0.128 ± 0.016	0.52TrpIle: 0.52 ± 0.032	0.539TrpLys: 0.539 ± 0.038	0.665TrpLeu: 0.665 ± 0.04	0.226TrpMet: 0.226 ± 0.021	0.462TrpAsn: 0.462 ± 0.03	0.115TrpPro: 0.115 ± 0.015	0.316TrpGln: 0.316 ± 0.027	0.318TrpArg: 0.318 ± 0.026	0.539TrpSer: 0.539 ± 0.033	0.383TrpThr: 0.383 ± 0.03	0.473TrpVal: 0.473 ± 0.034	0.11TrpTrp: 0.11 ± 0.017	0.352TrpTyr: 0.352 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.624TyrAla: 2.624 ± 0.073	0.739TyrCys: 0.739 ± 0.041	3.031TyrAsp: 3.031 ± 0.086	2.512TyrGlu: 2.512 ± 0.069	2.078TyrPhe: 2.078 ± 0.062	2.826TyrGly: 2.826 ± 0.083	0.688TyrHis: 0.688 ± 0.034	3.193TyrIle: 3.193 ± 0.086	3.077TyrLys: 3.077 ± 0.08	3.217TyrLeu: 3.217 ± 0.088	0.909TyrMet: 0.909 ± 0.038	2.565TyrAsn: 2.565 ± 0.076	1.4TyrPro: 1.4 ± 0.049	1.112TyrGln: 1.112 ± 0.043	1.431TyrArg: 1.431 ± 0.055	3.069TyrSer: 3.069 ± 0.091	2.481TyrThr: 2.481 ± 0.072	2.549TyrVal: 2.549 ± 0.082	0.327TyrTrp: 0.327 ± 0.025	1.928TyrTyr: 1.928 ± 0.072	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1647 proteins (547691 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---