Hubei diptera virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEK9|A0A1L3KEK9_9VIRU C2H2-type domain-containing protein OS=Hubei diptera virus 12 OX=1922873 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 5.47ARR4 pKa = 11.84NCFSSLSWTIPEE16 pKa = 4.33DD17 pKa = 3.52FCTFTHH23 pKa = 6.3YY24 pKa = 10.71LRR26 pKa = 11.84ALDD29 pKa = 3.99RR30 pKa = 11.84LEE32 pKa = 4.37MSSSPGYY39 pKa = 9.71PYY41 pKa = 9.89MRR43 pKa = 11.84RR44 pKa = 11.84APTNRR49 pKa = 11.84DD50 pKa = 3.02LFKK53 pKa = 11.06VDD55 pKa = 4.69DD56 pKa = 4.29VGNKK60 pKa = 9.91NSDD63 pKa = 3.27VVLIFWDD70 pKa = 3.38IVRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84LEE77 pKa = 4.0EE78 pKa = 4.93LGDD81 pKa = 4.04ADD83 pKa = 5.77PIRR86 pKa = 11.84LFIKK90 pKa = 10.29QEE92 pKa = 3.51PHH94 pKa = 5.41KK95 pKa = 10.53QKK97 pKa = 10.98KK98 pKa = 10.03LDD100 pKa = 3.87DD101 pKa = 3.61GRR103 pKa = 11.84FRR105 pKa = 11.84LISSVSVVDD114 pKa = 4.52QIIDD118 pKa = 3.3HH119 pKa = 6.08MLFAEE124 pKa = 5.01MNDD127 pKa = 2.98QMTQNWIRR135 pKa = 11.84NPIKK139 pKa = 10.57IGWTPVKK146 pKa = 10.3GGWRR150 pKa = 11.84AFPPGKK156 pKa = 9.75RR157 pKa = 11.84VAVDD161 pKa = 3.24KK162 pKa = 10.77SHH164 pKa = 6.98WDD166 pKa = 3.2WTVSLWMLDD175 pKa = 3.04CVLQLRR181 pKa = 11.84GEE183 pKa = 4.32LCVTRR188 pKa = 11.84GEE190 pKa = 5.66LFDD193 pKa = 4.3KK194 pKa = 10.46WLLLAKK200 pKa = 10.06YY201 pKa = 9.51RR202 pKa = 11.84YY203 pKa = 9.63SEE205 pKa = 4.79LFDD208 pKa = 4.08GPLFITSGGVLLRR221 pKa = 11.84QKK223 pKa = 10.75VPGVQKK229 pKa = 10.45SGCVNTLADD238 pKa = 3.89NSLMQWILHH247 pKa = 5.26ARR249 pKa = 11.84VMLEE253 pKa = 3.86HH254 pKa = 7.01GLNPHH259 pKa = 6.81DD260 pKa = 3.53VDD262 pKa = 3.48MWVMGDD268 pKa = 3.99DD269 pKa = 3.97TSQSAIVKK277 pKa = 10.1DD278 pKa = 3.96YY279 pKa = 11.05IDD281 pKa = 3.72WLGEE285 pKa = 3.88YY286 pKa = 10.08CVVKK290 pKa = 10.36EE291 pKa = 4.07SQIAPEE297 pKa = 3.89FCGFRR302 pKa = 11.84FMEE305 pKa = 4.74AGRR308 pKa = 11.84VEE310 pKa = 4.01PLYY313 pKa = 10.61KK314 pKa = 10.41GKK316 pKa = 9.54HH317 pKa = 5.09AFNLLHH323 pKa = 6.81MDD325 pKa = 4.22DD326 pKa = 5.64KK327 pKa = 11.32YY328 pKa = 11.38ATDD331 pKa = 3.37IANSYY336 pKa = 9.83SLLYY340 pKa = 10.3HH341 pKa = 7.17RR342 pKa = 11.84STHH345 pKa = 6.11RR346 pKa = 11.84DD347 pKa = 2.32WMRR350 pKa = 11.84NLFISMGINIYY361 pKa = 10.05PSYY364 pKa = 10.85YY365 pKa = 10.35LDD367 pKa = 3.86TIYY370 pKa = 11.21DD371 pKa = 3.73GVEE374 pKa = 3.49

Molecular weight: 43.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KER4|A0A1L3KER4_9VIRU C2H2-type domain-containing protein OS=Hubei diptera virus 12 OX=1922873 PE=4 SV=1
MM1 pKa = 7.85DD2 pKa = 3.83TQNNDD7 pKa = 2.36THH9 pKa = 6.97RR10 pKa = 11.84RR11 pKa = 11.84QTLGPTNVVVGWILDD26 pKa = 3.76IFWPLIPVFKK36 pKa = 9.91MLNVVCKK43 pKa = 9.96VCVVYY48 pKa = 10.75ALAVAFTGVYY58 pKa = 10.23RR59 pKa = 11.84LVLWISRR66 pKa = 11.84TPAQVTMNVTVFVTKK81 pKa = 10.63LFHH84 pKa = 6.98RR85 pKa = 11.84LVLLVHH91 pKa = 6.22WPIEE95 pKa = 4.05KK96 pKa = 10.21LVEE99 pKa = 4.01LMFMEE104 pKa = 5.31PEE106 pKa = 4.34PEE108 pKa = 5.14PIPLTWTEE116 pKa = 3.92TLLEE120 pKa = 4.1GLEE123 pKa = 4.19TKK125 pKa = 9.82IRR127 pKa = 11.84SILPVVAMITQSVVVYY143 pKa = 10.06IVGSFIASYY152 pKa = 10.99LLYY155 pKa = 10.53RR156 pKa = 11.84LTKK159 pKa = 10.31KK160 pKa = 9.84PVKK163 pKa = 8.69TLVMRR168 pKa = 11.84SRR170 pKa = 11.84GVYY173 pKa = 9.01VGEE176 pKa = 4.02AMRR179 pKa = 11.84EE180 pKa = 4.0GSQFVPVKK188 pKa = 10.36SIPPGQVSIMKK199 pKa = 10.29SGLLTDD205 pKa = 3.03SHH207 pKa = 8.06IGYY210 pKa = 9.84GLRR213 pKa = 11.84VHH215 pKa = 7.28DD216 pKa = 4.61CLVTPAHH223 pKa = 6.03VADD226 pKa = 4.2LEE228 pKa = 4.55KK229 pKa = 10.59TFVLANRR236 pKa = 11.84GVKK239 pKa = 10.05VLFTPTTRR247 pKa = 11.84IPSRR251 pKa = 11.84VIPDD255 pKa = 3.74LVYY258 pKa = 10.56IPVEE262 pKa = 3.65PSIWARR268 pKa = 11.84LGATAVKK275 pKa = 8.6MQRR278 pKa = 11.84GVPKK282 pKa = 10.33LANQVTCTGLPGATTGLLRR301 pKa = 11.84STTRR305 pKa = 11.84MGVYY309 pKa = 9.81SYY311 pKa = 11.14SGSTLPGMSGAGYY324 pKa = 9.88FVGNCCFGMHH334 pKa = 6.89TGNIAGEE341 pKa = 4.23NVGIASSLIMRR352 pKa = 11.84EE353 pKa = 3.6WRR355 pKa = 11.84CIYY358 pKa = 10.29KK359 pKa = 10.68GEE361 pKa = 4.11SSPDD365 pKa = 3.49FVDD368 pKa = 3.37TSVPIVKK375 pKa = 8.68KK376 pKa = 8.56TWDD379 pKa = 3.7DD380 pKa = 3.87VEE382 pKa = 4.73IDD384 pKa = 3.6AAATKK389 pKa = 10.3AWLEE393 pKa = 4.88DD394 pKa = 3.25EE395 pKa = 5.74DD396 pKa = 4.02FWEE399 pKa = 5.13DD400 pKa = 4.22RR401 pKa = 11.84VANWGQKK408 pKa = 9.61SFAEE412 pKa = 3.95NAFITEE418 pKa = 4.18EE419 pKa = 4.3TVVKK423 pKa = 10.47QQGNDD428 pKa = 3.69GEE430 pKa = 4.45EE431 pKa = 3.73KK432 pKa = 10.25VYY434 pKa = 9.77RR435 pKa = 11.84TTVVPSDD442 pKa = 3.24LTQRR446 pKa = 11.84VSLLEE451 pKa = 3.65QAMAKK456 pKa = 10.09VGLVKK461 pKa = 10.41CAQCDD466 pKa = 3.42EE467 pKa = 4.45VFVGKK472 pKa = 7.99TLEE475 pKa = 3.96QHH477 pKa = 6.48KK478 pKa = 10.05KK479 pKa = 9.3DD480 pKa = 3.22HH481 pKa = 6.44AVRR484 pKa = 11.84HH485 pKa = 5.76PCEE488 pKa = 3.87QCDD491 pKa = 3.93MVCLTVEE498 pKa = 3.86KK499 pKa = 10.49LSRR502 pKa = 11.84HH503 pKa = 4.41VKK505 pKa = 8.75EE506 pKa = 3.6FHH508 pKa = 7.14SRR510 pKa = 11.84VEE512 pKa = 4.16CDD514 pKa = 2.84RR515 pKa = 11.84CSTVCKK521 pKa = 10.88NMDD524 pKa = 3.16KK525 pKa = 10.74LANHH529 pKa = 7.35RR530 pKa = 11.84KK531 pKa = 8.13NCKK534 pKa = 9.87AVGDD538 pKa = 4.08VQLMEE543 pKa = 4.54VPSSSTHH550 pKa = 5.6PRR552 pKa = 11.84TPLVEE557 pKa = 3.98THH559 pKa = 6.41PQVPVAEE566 pKa = 4.36EE567 pKa = 4.03VKK569 pKa = 10.42EE570 pKa = 4.08SAFAIDD576 pKa = 3.53SRR578 pKa = 11.84KK579 pKa = 9.55LVKK582 pKa = 10.61NSPFLGQRR590 pKa = 11.84QPSRR594 pKa = 11.84TKK596 pKa = 10.06NSKK599 pKa = 9.79RR600 pKa = 11.84SQSGSSLKK608 pKa = 10.42VASPHH613 pKa = 6.41SPSQGEE619 pKa = 4.24MLSQILTSQKK629 pKa = 10.91SMQEE633 pKa = 3.67SFEE636 pKa = 4.65RR637 pKa = 11.84FLQVMVGQVSVTKK650 pKa = 10.76QNN652 pKa = 3.12

Molecular weight: 72.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1488

374

652

496.0

55.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.847 ± 0.82

2.352 ± 0.16

5.511 ± 1.09

4.839 ± 0.661

3.427 ± 0.418

6.317 ± 0.254

2.755 ± 0.16

4.503 ± 0.358

5.04 ± 0.698

8.602 ± 0.52

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.957 ± 0.523

3.091 ± 0.212

5.04 ± 0.28

4.234 ± 0.483

5.847 ± 0.343

7.93 ± 0.768

6.855 ± 1.065

10.013 ± 1.295

2.083 ± 0.451

2.755 ± 0.451
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski