Little cherry virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Velarivirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O40961|O40961_9CLOS Uncharacterized protein OS=Little cherry virus 1 OX=217686 PE=4 SV=1
MM1 pKa = 7.86PNLGSDD7 pKa = 3.81SVFDD11 pKa = 4.18EE12 pKa = 4.59YY13 pKa = 11.7SLVMEE18 pKa = 5.01DD19 pKa = 3.43RR20 pKa = 11.84PFNVFPARR28 pKa = 11.84PSLSSVHH35 pKa = 6.07AVNDD39 pKa = 4.0FMDD42 pKa = 3.66LTFNGLSSVDD52 pKa = 3.41YY53 pKa = 9.02LTRR56 pKa = 11.84TLRR59 pKa = 11.84FEE61 pKa = 4.27YY62 pKa = 10.72SDD64 pKa = 3.75IEE66 pKa = 4.4LPCLEE71 pKa = 5.39DD72 pKa = 5.32LLLTPTKK79 pKa = 10.24SKK81 pKa = 10.32PYY83 pKa = 9.73QAVNCKK89 pKa = 10.06VPILIGKK96 pKa = 7.52GEE98 pKa = 4.27RR99 pKa = 11.84SRR101 pKa = 11.84PDD103 pKa = 2.47TWKK106 pKa = 10.59QVILSLSKK114 pKa = 10.92RR115 pKa = 11.84NFAAPRR121 pKa = 11.84LNEE124 pKa = 5.19DD125 pKa = 2.83ISIDD129 pKa = 3.47EE130 pKa = 4.22TANRR134 pKa = 11.84LLTSLMRR141 pKa = 11.84CMEE144 pKa = 4.66PSRR147 pKa = 11.84LAEE150 pKa = 4.0YY151 pKa = 10.5FDD153 pKa = 4.15VVEE156 pKa = 4.49PDD158 pKa = 3.36VNKK161 pKa = 10.36INSWLLTRR169 pKa = 11.84DD170 pKa = 2.84RR171 pKa = 11.84RR172 pKa = 11.84KK173 pKa = 10.2YY174 pKa = 10.57GNVLRR179 pKa = 11.84GFDD182 pKa = 3.84GNDD185 pKa = 2.67WTTKK189 pKa = 10.44LFNLKK194 pKa = 10.57LMVKK198 pKa = 10.58GDD200 pKa = 4.33MKK202 pKa = 10.8PKK204 pKa = 10.33LDD206 pKa = 3.44MSGVSEE212 pKa = 4.38YY213 pKa = 11.24SPASNIIYY221 pKa = 8.44YY222 pKa = 8.24QTMINMFFSPIFLEE236 pKa = 3.56IFGRR240 pKa = 11.84IKK242 pKa = 10.71YY243 pKa = 10.17CLGDD247 pKa = 3.66KK248 pKa = 10.72VILFSGMNLDD258 pKa = 3.73EE259 pKa = 4.97MGDD262 pKa = 3.7LLEE265 pKa = 5.72SRR267 pKa = 11.84LCYY270 pKa = 9.83PLNSYY275 pKa = 11.06NFVEE279 pKa = 4.21VDD281 pKa = 3.99FSKK284 pKa = 10.7FDD286 pKa = 3.23KK287 pKa = 11.13SQGHH291 pKa = 5.4VIKK294 pKa = 10.59LYY296 pKa = 10.74EE297 pKa = 4.0EE298 pKa = 4.01LVYY301 pKa = 10.67KK302 pKa = 10.54IFKK305 pKa = 10.39FSPNLYY311 pKa = 10.7DD312 pKa = 3.53NFKK315 pKa = 10.58LSEE318 pKa = 4.22YY319 pKa = 10.0FCRR322 pKa = 11.84ASATCGVNLDD332 pKa = 4.88LYY334 pKa = 8.75CQRR337 pKa = 11.84RR338 pKa = 11.84TGSPNTWLSNSLATLAMLASVYY360 pKa = 10.95DD361 pKa = 4.34LDD363 pKa = 7.01DD364 pKa = 4.14IDD366 pKa = 6.11LIIVSGDD373 pKa = 3.18DD374 pKa = 3.23SLIISRR380 pKa = 11.84KK381 pKa = 9.13EE382 pKa = 3.63IEE384 pKa = 4.42NKK386 pKa = 10.21CLEE389 pKa = 4.18INNDD393 pKa = 3.05FGMDD397 pKa = 3.38AKK399 pKa = 10.76FLANPVPYY407 pKa = 9.7FCSKK411 pKa = 10.55FIIQVDD417 pKa = 3.27NRR419 pKa = 11.84IRR421 pKa = 11.84LVPDD425 pKa = 3.63PVRR428 pKa = 11.84FFEE431 pKa = 4.54KK432 pKa = 10.51LSTPVTLVQLEE443 pKa = 4.56HH444 pKa = 6.44PTLLRR449 pKa = 11.84EE450 pKa = 4.11RR451 pKa = 11.84FTSYY455 pKa = 10.73RR456 pKa = 11.84DD457 pKa = 3.36LMGSYY462 pKa = 10.25FDD464 pKa = 4.18EE465 pKa = 4.15NVIIAVEE472 pKa = 4.08RR473 pKa = 11.84FVSLKK478 pKa = 10.38YY479 pKa = 8.06NTPMGSGYY487 pKa = 11.01AAFCYY492 pKa = 9.66IHH494 pKa = 7.1CLLSSFKK501 pKa = 11.01NFLTIFGDD509 pKa = 3.65DD510 pKa = 3.41TSIQLL515 pKa = 3.8

Molecular weight: 59.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O40962|O40962_9CLOS Putative viral coat protein OS=Little cherry virus 1 OX=217686 PE=4 SV=1
MM1 pKa = 7.19VVLVWVCLISVVCFFVSPSNIGLEE25 pKa = 4.02KK26 pKa = 10.25FSRR29 pKa = 11.84FGFSNVCC36 pKa = 3.3

Molecular weight: 4.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

5475

36

2301

608.3

69.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.731 ± 0.762

1.918 ± 0.189

6.557 ± 0.34

5.352 ± 0.54

6.393 ± 0.263

4.493 ± 0.344

2.009 ± 0.255

7.306 ± 0.403

7.635 ± 0.413

9.991 ± 0.552

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.295

7.123 ± 0.514

3.087 ± 0.286

2.301 ± 0.219

4.329 ± 0.239

8.402 ± 0.362

5.205 ± 0.369

6.74 ± 0.467

0.53 ± 0.115

3.799 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski