Methanospirillum lacunae

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanospirillaceae; Methanospirillum

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3377 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V2NFG1|A0A2V2NFG1_9EURY Methyl-accepting chemotaxis protein OS=Methanospirillum lacunae OX=668570 GN=DK846_04065 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.87PIYY5 pKa = 10.24GYY7 pKa = 10.25IFTIVVFVCFIVGFGIADD25 pKa = 3.61NEE27 pKa = 4.16QSMGTSPDD35 pKa = 3.14IPPTTNALSKK45 pKa = 9.6TDD47 pKa = 3.56VVSPSGDD54 pKa = 3.48STGGTDD60 pKa = 3.64SSHH63 pKa = 5.85EE64 pKa = 4.4TTSTTDD70 pKa = 2.8TGSADD75 pKa = 3.41SSSTGQTQTGEE86 pKa = 4.19ITSTTQQSTDD96 pKa = 3.24GLSEE100 pKa = 4.13TGSSSEE106 pKa = 4.31TSSSTTEE113 pKa = 3.73TGSADD118 pKa = 3.15SSTPGQTQTGEE129 pKa = 4.13ITSTTQQSTDD139 pKa = 3.24GLSEE143 pKa = 4.13TGSSSEE149 pKa = 4.31TSSSTTEE156 pKa = 3.79TRR158 pKa = 11.84SADD161 pKa = 3.13ASTTGDD167 pKa = 3.2TTTGQNPSTTEE178 pKa = 3.66HH179 pKa = 5.41TTGDD183 pKa = 3.29VSKK186 pKa = 10.98KK187 pKa = 10.4VGSSLNTASGDD198 pKa = 3.62QTVLNQDD205 pKa = 3.92DD206 pKa = 4.47GTTTSQTVIITPHH219 pKa = 6.38SDD221 pKa = 3.24NDD223 pKa = 3.63EE224 pKa = 4.4KK225 pKa = 11.48SPEE228 pKa = 4.28TVTLGTVTVQSIIDD242 pKa = 3.77LTPSGGTGYY251 pKa = 9.04TYY253 pKa = 11.24DD254 pKa = 4.1STTSSYY260 pKa = 11.34DD261 pKa = 2.84ITGAGNTYY269 pKa = 10.17VLQSGFSTTSTDD281 pKa = 3.51FAIRR285 pKa = 11.84IQASNVILDD294 pKa = 4.05GNYY297 pKa = 7.73QTITGSGGSAGVSVISGTDD316 pKa = 2.99SVTVRR321 pKa = 11.84NFGGINYY328 pKa = 8.18FNNGINSQGDD338 pKa = 3.58STTIFGNTASEE349 pKa = 4.05NNYY352 pKa = 10.49GIVSTGDD359 pKa = 3.05SATISGNTANDD370 pKa = 3.13NGFAGIKK377 pKa = 9.68SDD379 pKa = 3.45GVSAVISGNTANSNDD394 pKa = 3.64EE395 pKa = 4.82GIEE398 pKa = 4.24STGPTATISSNTAEE412 pKa = 4.17NNNNNGINSDD422 pKa = 4.01GEE424 pKa = 4.38SATIRR429 pKa = 11.84SNTVNSNGIAGSDD442 pKa = 3.75CNGIDD447 pKa = 3.85SDD449 pKa = 4.36GNYY452 pKa = 10.66AQISGNIASNNNNNGVDD469 pKa = 4.02SIGVSDD475 pKa = 4.09VIIGNEE481 pKa = 3.77VNNNGKK487 pKa = 10.11SGIYY491 pKa = 9.64SAAPNAFIGTNTADD505 pKa = 3.77GNTQTGIDD513 pKa = 4.03SEE515 pKa = 4.53QTSATISGNTVSNNNYY531 pKa = 9.8GIYY534 pKa = 10.27SAGSDD539 pKa = 3.31AAISSNTANRR549 pKa = 11.84NTIGIVSYY557 pKa = 11.13GDD559 pKa = 3.26SAAINDD565 pKa = 3.56NTANSNNNNGIYY577 pKa = 9.97SDD579 pKa = 4.4GEE581 pKa = 4.3SVTIKK586 pKa = 10.83GNTVAEE592 pKa = 4.29NTNSGLVLGGGSGSVYY608 pKa = 10.71NNKK611 pKa = 9.26FANTNNVGGVSNPSGYY627 pKa = 7.89TWNQAPSPGSNVVGGPNIAGNYY649 pKa = 7.87WSDD652 pKa = 3.4PTGTGWSDD660 pKa = 2.84THH662 pKa = 6.5TPTTTGYY669 pKa = 6.21TTEE672 pKa = 4.31RR673 pKa = 11.84YY674 pKa = 9.17NVGGSMYY681 pKa = 11.16DD682 pKa = 3.25NAPLVKK688 pKa = 9.39TRR690 pKa = 11.84SSPPSPDD697 pKa = 3.77PNPDD701 pKa = 3.46YY702 pKa = 10.96PPSHH706 pKa = 7.47PNQPSDD712 pKa = 3.43PSSRR716 pKa = 11.84SLINIVPNSADD727 pKa = 2.91LSFSVEE733 pKa = 4.06GNQATITLDD742 pKa = 3.41LEE744 pKa = 4.8NIGTVTLSPSARR756 pKa = 11.84IVLVPINDD764 pKa = 3.81PAIPIGVLPASYY776 pKa = 11.02DD777 pKa = 3.42SGKK780 pKa = 10.38YY781 pKa = 10.2LINFGVQIPSSPGTYY796 pKa = 9.08TYY798 pKa = 10.38TFQPEE803 pKa = 4.76MITTDD808 pKa = 3.41PSGNEE813 pKa = 3.64VLMNAGGLVTFIVTVNPDD831 pKa = 2.96GTVNCTLGSS840 pKa = 3.59

Molecular weight: 85.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V2MV55|A0A2V2MV55_9EURY Uncharacterized protein OS=Methanospirillum lacunae OX=668570 GN=DK846_10345 PE=4 SV=1
MM1 pKa = 7.38SKK3 pKa = 10.34VSKK6 pKa = 10.03CRR8 pKa = 11.84KK9 pKa = 8.62IRR11 pKa = 11.84LSKK14 pKa = 9.64KK15 pKa = 6.56TQQNRR20 pKa = 11.84RR21 pKa = 11.84VPQWVMIKK29 pKa = 8.95TKK31 pKa = 10.08RR32 pKa = 11.84KK33 pKa = 10.07VMTHH37 pKa = 6.13PARR40 pKa = 11.84HH41 pKa = 4.6SWRR44 pKa = 11.84RR45 pKa = 11.84STLKK49 pKa = 10.66VV50 pKa = 3.07

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3377

0

3377

1080887

31

2928

320.1

35.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.904 ± 0.047

1.332 ± 0.019

5.641 ± 0.035

6.231 ± 0.046

3.954 ± 0.03

7.382 ± 0.039

2.025 ± 0.02

8.638 ± 0.046

5.102 ± 0.039

9.152 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.021

4.167 ± 0.049

4.488 ± 0.03

3.276 ± 0.024

4.708 ± 0.042

7.304 ± 0.047

6.006 ± 0.047

6.825 ± 0.032

1.03 ± 0.015

3.302 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski