Amino acid dipepetide frequency for Methanospirillum lacunae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.759AlaAla: 5.759 ± 0.102	0.98AlaCys: 0.98 ± 0.036	4.234AlaAsp: 4.234 ± 0.073	4.43AlaGlu: 4.43 ± 0.068	2.59AlaPhe: 2.59 ± 0.055	6.156AlaGly: 6.156 ± 0.088	1.315AlaHis: 1.315 ± 0.035	5.854AlaIle: 5.854 ± 0.081	3.073AlaLys: 3.073 ± 0.063	6.784AlaLeu: 6.784 ± 0.098	1.986AlaMet: 1.986 ± 0.051	2.175AlaAsn: 2.175 ± 0.048	2.244AlaPro: 2.244 ± 0.055	1.854AlaGln: 1.854 ± 0.044	3.246AlaArg: 3.246 ± 0.062	4.466AlaSer: 4.466 ± 0.072	3.717AlaThr: 3.717 ± 0.072	5.338AlaVal: 5.338 ± 0.083	0.77AlaTrp: 0.77 ± 0.028	2.071AlaTyr: 2.071 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.848CysAla: 0.848 ± 0.033	0.279CysCys: 0.279 ± 0.017	0.699CysAsp: 0.699 ± 0.024	0.628CysGlu: 0.628 ± 0.023	0.471CysPhe: 0.471 ± 0.018	1.384CysGly: 1.384 ± 0.04	0.3CysHis: 0.3 ± 0.017	1.045CysIle: 1.045 ± 0.033	0.532CysLys: 0.532 ± 0.021	1.092CysLeu: 1.092 ± 0.037	0.334CysMet: 0.334 ± 0.018	0.582CysAsn: 0.582 ± 0.023	0.835CysPro: 0.835 ± 0.03	0.398CysGln: 0.398 ± 0.02	0.708CysArg: 0.708 ± 0.027	1.018CysSer: 1.018 ± 0.04	0.836CysThr: 0.836 ± 0.03	0.787CysVal: 0.787 ± 0.032	0.153CysTrp: 0.153 ± 0.01	0.389CysTyr: 0.389 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.926AspAla: 3.926 ± 0.07	0.612AspCys: 0.612 ± 0.02	2.827AspAsp: 2.827 ± 0.059	3.917AspGlu: 3.917 ± 0.066	2.06AspPhe: 2.06 ± 0.05	4.08AspGly: 4.08 ± 0.088	1.269AspHis: 1.269 ± 0.037	5.124AspIle: 5.124 ± 0.081	2.473AspLys: 2.473 ± 0.053	6.307AspLeu: 6.307 ± 0.077	1.248AspMet: 1.248 ± 0.037	2.044AspAsn: 2.044 ± 0.042	3.148AspPro: 3.148 ± 0.048	2.261AspGln: 2.261 ± 0.047	2.711AspArg: 2.711 ± 0.06	3.472AspSer: 3.472 ± 0.069	3.028AspThr: 3.028 ± 0.066	3.547AspVal: 3.547 ± 0.068	0.57AspTrp: 0.57 ± 0.024	1.79AspTyr: 1.79 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	4.253GluAla: 4.253 ± 0.079	0.644GluCys: 0.644 ± 0.027	3.067GluAsp: 3.067 ± 0.052	4.862GluGlu: 4.862 ± 0.086	2.189GluPhe: 2.189 ± 0.046	3.852GluGly: 3.852 ± 0.067	1.276GluHis: 1.276 ± 0.042	6.115GluIle: 6.115 ± 0.089	4.647GluLys: 4.647 ± 0.076	5.051GluLeu: 5.051 ± 0.085	1.922GluMet: 1.922 ± 0.044	2.783GluAsn: 2.783 ± 0.06	2.125GluPro: 2.125 ± 0.05	2.4GluGln: 2.4 ± 0.056	3.14GluArg: 3.14 ± 0.062	3.824GluSer: 3.824 ± 0.07	3.307GluThr: 3.307 ± 0.058	4.072GluVal: 4.072 ± 0.073	0.606GluTrp: 0.606 ± 0.029	2.174GluTyr: 2.174 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	2.581PheAla: 2.581 ± 0.051	0.644PheCys: 0.644 ± 0.024	2.176PheAsp: 2.176 ± 0.046	2.115PheGlu: 2.115 ± 0.047	2.084PhePhe: 2.084 ± 0.06	3.059PheGly: 3.059 ± 0.064	0.808PheHis: 0.808 ± 0.027	3.243PheIle: 3.243 ± 0.065	1.534PheLys: 1.534 ± 0.036	3.894PheLeu: 3.894 ± 0.076	0.831PheMet: 0.831 ± 0.034	1.602PheAsn: 1.602 ± 0.053	1.671PhePro: 1.671 ± 0.042	1.163PheGln: 1.163 ± 0.033	1.882PheArg: 1.882 ± 0.048	3.622PheSer: 3.622 ± 0.06	2.47PheThr: 2.47 ± 0.054	2.405PheVal: 2.405 ± 0.052	0.496PheTrp: 0.496 ± 0.026	1.265PheTyr: 1.265 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	4.625GlyAla: 4.625 ± 0.086	1.192GlyCys: 1.192 ± 0.037	4.178GlyAsp: 4.178 ± 0.086	4.323GlyGlu: 4.323 ± 0.073	2.993GlyPhe: 2.993 ± 0.06	5.063GlyGly: 5.063 ± 0.094	1.274GlyHis: 1.274 ± 0.037	7.017GlyIle: 7.017 ± 0.093	4.713GlyLys: 4.713 ± 0.083	6.101GlyLeu: 6.101 ± 0.083	2.292GlyMet: 2.292 ± 0.048	3.072GlyAsn: 3.072 ± 0.07	2.282GlyPro: 2.282 ± 0.058	1.915GlyGln: 1.915 ± 0.048	3.246GlyArg: 3.246 ± 0.067	5.457GlySer: 5.457 ± 0.092	4.857GlyThr: 4.857 ± 0.077	5.348GlyVal: 5.348 ± 0.069	0.859GlyTrp: 0.859 ± 0.026	3.017GlyTyr: 3.017 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	1.428HisAla: 1.428 ± 0.039	0.301HisCys: 0.301 ± 0.018	1.248HisAsp: 1.248 ± 0.036	1.324HisGlu: 1.324 ± 0.038	0.713HisPhe: 0.713 ± 0.028	1.441HisGly: 1.441 ± 0.041	0.619HisHis: 0.619 ± 0.023	1.579HisIle: 1.579 ± 0.038	0.753HisLys: 0.753 ± 0.026	2.119HisLeu: 2.119 ± 0.049	0.439HisMet: 0.439 ± 0.025	0.736HisAsn: 0.736 ± 0.028	1.352HisPro: 1.352 ± 0.034	0.795HisGln: 0.795 ± 0.026	1.015HisArg: 1.015 ± 0.035	1.186HisSer: 1.186 ± 0.036	1.168HisThr: 1.168 ± 0.041	1.245HisVal: 1.245 ± 0.033	0.157HisTrp: 0.157 ± 0.012	0.637HisTyr: 0.637 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.125IleAla: 6.125 ± 0.08	1.274IleCys: 1.274 ± 0.038	4.462IleAsp: 4.462 ± 0.084	4.9IleGlu: 4.9 ± 0.08	3.39IlePhe: 3.39 ± 0.078	6.012IleGly: 6.012 ± 0.085	1.772IleHis: 1.772 ± 0.047	7.237IleIle: 7.237 ± 0.11	3.833IleLys: 3.833 ± 0.076	8.487IleLeu: 8.487 ± 0.121	1.7IleMet: 1.7 ± 0.045	3.376IleAsn: 3.376 ± 0.078	4.842IlePro: 4.842 ± 0.072	2.935IleGln: 2.935 ± 0.06	4.745IleArg: 4.745 ± 0.075	7.452IleSer: 7.452 ± 0.098	5.927IleThr: 5.927 ± 0.085	4.921IleVal: 4.921 ± 0.072	0.843IleTrp: 0.843 ± 0.031	2.15IleTyr: 2.15 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	3.431LysAla: 3.431 ± 0.067	0.483LysCys: 0.483 ± 0.022	2.865LysAsp: 2.865 ± 0.056	3.915LysGlu: 3.915 ± 0.072	1.589LysPhe: 1.589 ± 0.043	3.555LysGly: 3.555 ± 0.057	0.79LysHis: 0.79 ± 0.029	4.488LysIle: 4.488 ± 0.081	3.822LysLys: 3.822 ± 0.075	3.341LysLeu: 3.341 ± 0.053	1.393LysMet: 1.393 ± 0.041	2.876LysAsn: 2.876 ± 0.059	2.313LysPro: 2.313 ± 0.053	1.696LysGln: 1.696 ± 0.048	2.366LysArg: 2.366 ± 0.05	3.266LysSer: 3.266 ± 0.052	3.421LysThr: 3.421 ± 0.064	3.16LysVal: 3.16 ± 0.061	0.482LysTrp: 0.482 ± 0.025	1.768LysTyr: 1.768 ± 0.042	0.0LysXaa: 0.0 ± 0.0
Leu	6.379LeuAla: 6.379 ± 0.079	1.216LeuCys: 1.216 ± 0.039	4.809LeuAsp: 4.809 ± 0.077	5.069LeuGlu: 5.069 ± 0.076	4.325LeuPhe: 4.325 ± 0.079	5.789LeuGly: 5.789 ± 0.082	1.862LeuHis: 1.862 ± 0.043	8.442LeuIle: 8.442 ± 0.112	5.028LeuLys: 5.028 ± 0.073	8.888LeuLeu: 8.888 ± 0.125	2.31LeuMet: 2.31 ± 0.051	4.148LeuAsn: 4.148 ± 0.077	3.955LeuPro: 3.955 ± 0.065	2.799LeuGln: 2.799 ± 0.054	3.564LeuArg: 3.564 ± 0.067	7.62LeuSer: 7.62 ± 0.093	5.539LeuThr: 5.539 ± 0.069	6.257LeuVal: 6.257 ± 0.089	0.782LeuTrp: 0.782 ± 0.028	2.74LeuTyr: 2.74 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	1.929MetAla: 1.929 ± 0.045	0.224MetCys: 0.224 ± 0.015	1.49MetAsp: 1.49 ± 0.036	1.475MetGlu: 1.475 ± 0.042	0.76MetPhe: 0.76 ± 0.031	1.849MetGly: 1.849 ± 0.05	0.469MetHis: 0.469 ± 0.019	2.198MetIle: 2.198 ± 0.05	1.675MetLys: 1.675 ± 0.047	1.941MetLeu: 1.941 ± 0.045	0.762MetMet: 0.762 ± 0.032	1.449MetAsn: 1.449 ± 0.037	1.043MetPro: 1.043 ± 0.032	0.913MetGln: 0.913 ± 0.031	1.168MetArg: 1.168 ± 0.037	1.624MetSer: 1.624 ± 0.042	1.616MetThr: 1.616 ± 0.039	1.849MetVal: 1.849 ± 0.05	0.177MetTrp: 0.177 ± 0.017	0.693MetTyr: 0.693 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.824AsnAla: 2.824 ± 0.059	0.501AsnCys: 0.501 ± 0.024	1.799AsnAsp: 1.799 ± 0.043	2.426AsnGlu: 2.426 ± 0.055	1.397AsnPhe: 1.397 ± 0.038	2.988AsnGly: 2.988 ± 0.065	0.878AsnHis: 0.878 ± 0.03	3.301AsnIle: 3.301 ± 0.072	1.836AsnLys: 1.836 ± 0.048	4.367AsnLeu: 4.367 ± 0.076	0.899AsnMet: 0.899 ± 0.028	2.123AsnAsn: 2.123 ± 0.075	2.848AsnPro: 2.848 ± 0.061	1.888AsnGln: 1.888 ± 0.045	2.087AsnArg: 2.087 ± 0.049	2.901AsnSer: 2.901 ± 0.072	2.53AsnThr: 2.53 ± 0.06	2.409AsnVal: 2.409 ± 0.05	0.405AsnTrp: 0.405 ± 0.021	1.263AsnTyr: 1.263 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	3.145ProAla: 3.145 ± 0.062	0.533ProCys: 0.533 ± 0.024	3.929ProAsp: 3.929 ± 0.058	3.491ProGlu: 3.491 ± 0.059	1.836ProPhe: 1.836 ± 0.044	3.889ProGly: 3.889 ± 0.078	0.866ProHis: 0.866 ± 0.029	3.076ProIle: 3.076 ± 0.058	1.675ProLys: 1.675 ± 0.041	3.909ProLeu: 3.909 ± 0.067	0.898ProMet: 0.898 ± 0.031	1.394ProAsn: 1.394 ± 0.038	1.714ProPro: 1.714 ± 0.047	1.145ProGln: 1.145 ± 0.031	1.51ProArg: 1.51 ± 0.04	2.997ProSer: 2.997 ± 0.064	2.343ProThr: 2.343 ± 0.078	4.582ProVal: 4.582 ± 0.074	0.448ProTrp: 0.448 ± 0.021	1.498ProTyr: 1.498 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	2.434GlnAla: 2.434 ± 0.052	0.316GlnCys: 0.316 ± 0.02	1.735GlnAsp: 1.735 ± 0.037	2.189GlnGlu: 2.189 ± 0.052	1.314GlnPhe: 1.314 ± 0.042	2.108GlnGly: 2.108 ± 0.045	0.538GlnHis: 0.538 ± 0.022	3.109GlnIle: 3.109 ± 0.054	2.269GlnLys: 2.269 ± 0.05	2.149GlnLeu: 2.149 ± 0.044	1.05GlnMet: 1.05 ± 0.03	1.715GlnAsn: 1.715 ± 0.044	1.104GlnPro: 1.104 ± 0.034	1.134GlnGln: 1.134 ± 0.039	1.339GlnArg: 1.339 ± 0.034	2.003GlnSer: 2.003 ± 0.046	2.035GlnThr: 2.035 ± 0.049	2.551GlnVal: 2.551 ± 0.05	0.311GlnTrp: 0.311 ± 0.018	1.359GlnTyr: 1.359 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.844ArgAla: 2.844 ± 0.061	0.695ArgCys: 0.695 ± 0.027	2.858ArgAsp: 2.858 ± 0.055	3.632ArgGlu: 3.632 ± 0.076	2.149ArgPhe: 2.149 ± 0.045	2.785ArgGly: 2.785 ± 0.053	0.948ArgHis: 0.948 ± 0.029	4.34ArgIle: 4.34 ± 0.081	2.615ArgLys: 2.615 ± 0.056	3.999ArgLeu: 3.999 ± 0.065	1.368ArgMet: 1.368 ± 0.035	1.949ArgAsn: 1.949 ± 0.052	1.54ArgPro: 1.54 ± 0.04	1.487ArgGln: 1.487 ± 0.042	2.392ArgArg: 2.392 ± 0.054	3.171ArgSer: 3.171 ± 0.067	2.565ArgThr: 2.565 ± 0.053	3.159ArgVal: 3.159 ± 0.049	0.524ArgTrp: 0.524 ± 0.025	2.058ArgTyr: 2.058 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	4.74SerAla: 4.74 ± 0.085	1.003SerCys: 1.003 ± 0.031	4.644SerAsp: 4.644 ± 0.072	4.425SerGlu: 4.425 ± 0.075	2.939SerPhe: 2.939 ± 0.058	6.803SerGly: 6.803 ± 0.104	1.665SerHis: 1.665 ± 0.041	5.611SerIle: 5.611 ± 0.083	2.76SerLys: 2.76 ± 0.067	6.911SerLeu: 6.911 ± 0.104	1.766SerMet: 1.766 ± 0.042	2.575SerAsn: 2.575 ± 0.06	3.336SerPro: 3.336 ± 0.066	2.29SerGln: 2.29 ± 0.053	3.568SerArg: 3.568 ± 0.06	5.99SerSer: 5.99 ± 0.116	3.887SerThr: 3.887 ± 0.073	5.003SerVal: 5.003 ± 0.072	0.814SerTrp: 0.814 ± 0.029	2.31SerTyr: 2.31 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	4.162ThrAla: 4.162 ± 0.064	0.767ThrCys: 0.767 ± 0.027	3.448ThrAsp: 3.448 ± 0.07	3.171ThrGlu: 3.171 ± 0.052	2.224ThrPhe: 2.224 ± 0.043	5.896ThrGly: 5.896 ± 0.085	1.167ThrHis: 1.167 ± 0.042	5.179ThrIle: 5.179 ± 0.078	2.466ThrLys: 2.466 ± 0.061	5.536ThrLeu: 5.536 ± 0.071	1.266ThrMet: 1.266 ± 0.037	2.261ThrAsn: 2.261 ± 0.052	3.389ThrPro: 3.389 ± 0.081	1.593ThrGln: 1.593 ± 0.042	2.739ThrArg: 2.739 ± 0.055	4.337ThrSer: 4.337 ± 0.078	3.513ThrThr: 3.513 ± 0.079	4.316ThrVal: 4.316 ± 0.09	0.686ThrTrp: 0.686 ± 0.027	1.947ThrTyr: 1.947 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	4.664ValAla: 4.664 ± 0.082	1.085ValCys: 1.085 ± 0.032	3.447ValAsp: 3.447 ± 0.06	3.598ValGlu: 3.598 ± 0.068	2.794ValPhe: 2.794 ± 0.053	4.119ValGly: 4.119 ± 0.074	1.411ValHis: 1.411 ± 0.034	6.166ValIle: 6.166 ± 0.075	3.424ValLys: 3.424 ± 0.066	6.225ValLeu: 6.225 ± 0.076	1.87ValMet: 1.87 ± 0.046	2.643ValAsn: 2.643 ± 0.068	3.184ValPro: 3.184 ± 0.066	2.177ValGln: 2.177 ± 0.051	3.41ValArg: 3.41 ± 0.072	5.587ValSer: 5.587 ± 0.078	4.766ValThr: 4.766 ± 0.074	4.84ValVal: 4.84 ± 0.089	0.672ValTrp: 0.672 ± 0.027	2.166ValTyr: 2.166 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.604TrpAla: 0.604 ± 0.028	0.103TrpCys: 0.103 ± 0.01	0.632TrpAsp: 0.632 ± 0.031	0.546TrpGlu: 0.546 ± 0.024	0.481TrpPhe: 0.481 ± 0.024	0.599TrpGly: 0.599 ± 0.025	0.195TrpHis: 0.195 ± 0.011	0.976TrpIle: 0.976 ± 0.032	0.674TrpLys: 0.674 ± 0.028	0.827TrpLeu: 0.827 ± 0.033	0.303TrpMet: 0.303 ± 0.019	0.758TrpAsn: 0.758 ± 0.03	0.266TrpPro: 0.266 ± 0.016	0.392TrpGln: 0.392 ± 0.025	0.501TrpArg: 0.501 ± 0.023	0.609TrpSer: 0.609 ± 0.025	0.528TrpThr: 0.528 ± 0.022	0.671TrpVal: 0.671 ± 0.023	0.169TrpTrp: 0.169 ± 0.013	0.468TrpTyr: 0.468 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.344TyrAla: 2.344 ± 0.048	0.467TyrCys: 0.467 ± 0.021	1.868TyrAsp: 1.868 ± 0.042	1.873TyrGlu: 1.873 ± 0.052	1.244TyrPhe: 1.244 ± 0.037	2.397TyrGly: 2.397 ± 0.056	0.832TyrHis: 0.832 ± 0.031	2.28TyrIle: 2.28 ± 0.047	1.22TyrLys: 1.22 ± 0.033	3.592TyrLeu: 3.592 ± 0.061	0.664TyrMet: 0.664 ± 0.027	1.408TyrAsn: 1.408 ± 0.038	1.688TyrPro: 1.688 ± 0.041	1.527TyrGln: 1.527 ± 0.039	1.742TyrArg: 1.742 ± 0.043	2.441TyrSer: 2.441 ± 0.056	2.009TyrThr: 2.009 ± 0.046	1.787TyrVal: 1.787 ± 0.044	0.377TyrTrp: 0.377 ± 0.021	1.262TyrTyr: 1.262 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3377 proteins (1080887 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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